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Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. aureus and E. faecalis.
Front Microbiol. 2019; 10:2453.FM

Abstract

Methicillin-resistant/susceptible Staphylococcus aureus (MRSA/MSSA) and Enterococcus faecalis strains are often found in community- and hospital-acquired infections. The single use of conventional antibiotics hardly completely kills the bacterial cells of interest, especially in the form of biofilms. Thus, drug repurposing and antimicrobial combination are promising ways to solve this problem. Antimicrobial susceptibility assays against cocci in a suspension and in a biofilm mode of growth were performed with broth microdilution methods. Checkerboard assays and the cutaneous mouse infection model were used to examine the activity of auranofin and conventional antibiotics alone and in combination. In the present study, auranofin possesses potent antimicrobial activities against both planktonic cells and biofilms with minimum inhibitory concentrations ranging 0.125-0.5 mg/L. Auranofin in combination with linezolid or fosfomycin showed synergistic antimicrobial activities against S. aureus MSSA and MRSA both in vitro and in vivo. Similarly, auranofin also behaved synergistic effect with chloramphenicol against E. faecalis. Additionally, auranofin improved the antibiofilm efficacy of chloramphenicol and linezolid, even on the biofilms grown on a catheter surface. Though, S. epidermidis showed significant susceptibility to AF treatment, no synergistic antimicrobial effects were observed with antibiotics we tested. In all, the use of a combination of auranofin with linezolid, fosfomycin, and chloramphenicol can provide a synergistic microbicidal effect in vitro and in vivo, which rapidly enhances antimicrobial activity and may help prevent or delay the emergence of resistance.

Authors+Show Affiliations

Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31708908

Citation

She, Pengfei, et al. "Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. Aureus and E. Faecalis." Frontiers in Microbiology, vol. 10, 2019, p. 2453.
She P, Zhou L, Li S, et al. Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. aureus and E. faecalis. Frontiers in microbiology. 2019;10:2453.
She, P., Zhou, L., Li, S., Liu, Y., Xu, L., Chen, L., Luo, Z., & Wu, Y. (2019). Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. aureus and E. faecalis. Frontiers in Microbiology, 10, 2453. https://doi.org/10.3389/fmicb.2019.02453
She P, et al. Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. Aureus and E. Faecalis. Frontiers in microbiology. 2019;10:2453. PubMed PMID: 31708908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic Microbicidal Effect of Auranofin and Antibiotics Against Planktonic and Biofilm-Encased S. aureus and E. faecalis. AU - She,Pengfei, AU - Zhou,Linying, AU - Li,Shijia, AU - Liu,Yiqing, AU - Xu,Lanlan, AU - Chen,Lihua, AU - Luo,Zhen, AU - Wu,Yong, Y1 - 2019/10/24/ PY - 2019/07/12/received PY - 2019/10/11/accepted PY - 2019/11/12/entrez PY - 2019/11/12/pubmed PY - 2019/11/12/medline KW - Enterococcus faecalis KW - Staphylococcus aureus KW - auranofin KW - biofilm KW - combination therapy KW - subcutaneous abscess model SP - 2453 EP - 2453 JF - Frontiers in microbiology VL - 10 N2 - Methicillin-resistant/susceptible Staphylococcus aureus (MRSA/MSSA) and Enterococcus faecalis strains are often found in community- and hospital-acquired infections. The single use of conventional antibiotics hardly completely kills the bacterial cells of interest, especially in the form of biofilms. Thus, drug repurposing and antimicrobial combination are promising ways to solve this problem. Antimicrobial susceptibility assays against cocci in a suspension and in a biofilm mode of growth were performed with broth microdilution methods. Checkerboard assays and the cutaneous mouse infection model were used to examine the activity of auranofin and conventional antibiotics alone and in combination. In the present study, auranofin possesses potent antimicrobial activities against both planktonic cells and biofilms with minimum inhibitory concentrations ranging 0.125-0.5 mg/L. Auranofin in combination with linezolid or fosfomycin showed synergistic antimicrobial activities against S. aureus MSSA and MRSA both in vitro and in vivo. Similarly, auranofin also behaved synergistic effect with chloramphenicol against E. faecalis. Additionally, auranofin improved the antibiofilm efficacy of chloramphenicol and linezolid, even on the biofilms grown on a catheter surface. Though, S. epidermidis showed significant susceptibility to AF treatment, no synergistic antimicrobial effects were observed with antibiotics we tested. In all, the use of a combination of auranofin with linezolid, fosfomycin, and chloramphenicol can provide a synergistic microbicidal effect in vitro and in vivo, which rapidly enhances antimicrobial activity and may help prevent or delay the emergence of resistance. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/31708908/Synergistic_Microbicidal_Effect_of_Auranofin_and_Antibiotics_Against_Planktonic_and_Biofilm_Encased_S__aureus_and_E__faecalis_ L2 - https://doi.org/10.3389/fmicb.2019.02453 DB - PRIME DP - Unbound Medicine ER -
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