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Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing.
PLoS One. 2019; 14(11):e0220089.Plos

Abstract

Powdery mildew is one of the most widespread diseases of wheat. The development and deployment of resistant varieties are one of the most economical and effective methods to manage this disease. Our previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred a high level of resistance to powdery mildew of wheat. In this study, resistance spectrum of TA7733 was assayed by using 15 Blumeria graminis f. sp. tritici (Bgt) isolates prevalent in different regions of China. The result indicated that TA7733 was highly resistant to all tested Bgt isolates and the gene(s) on chromosome 2Mb conferred broad-spectrum resistance to powdery mildew. In order to characterize mechanism of powdery mildew resistance by identifying candidates R-genes derived from Ae. biuncialis chromosome 2Mb and develop 2Mb-specific molecular markers, we performed RNA-seq analysis on TA7733 and CS. In total we identified 7,278 unigenes that showed specific expression in TA7733 pre and post Bgt-infection when compared to CS. Of these 7,278 unigenes, 295 were annotated as putative resistance (R) genes. Comparatively analysis of R-gene sequences from TA7733 and CS and integration CS Ref Seq v1.0 were used to develop R-gene specific primers. Of 295 R-genes we identified 53 R-genes were specific to 2Mb and could be involved in powdery mildew resistance. Functional annotation of majority of the 53 R-genes encoded nucleotide binding leucine rich repeat (NLR) protein. The broad-spectrum resistance to powdery mildew in TA7733 and availability of 2Mb-derived putative candidate R-gene specific molecular markers identified in this study will lay foundations for transferring powdery mildew resistance from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination. Our study also provides useful candidates for further isolation and cloning of powdery mildew resistance gene(s) from Ae. biuncialis chromosome 2Mb.

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Authors+Show Affiliations

College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan Province, China.

College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan Province, China.

College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan Province, China.

College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan Province, China.

Wheat Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province, China.

College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan Province, China.

College of Life Sciences, Yantai University, Yantai, Shandong Province, China.

College of Life Sciences, Henan Agricultural University, Zhengzhou, Henan Province, China.

MeSH

AegilopsAscomycotaChromosomes, PlantDisease ResistancePlant DiseasesPlant ProteinsSequence Analysis, RNATranscriptome

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31710598

Citation

Li, Huanhuan, et al. "Discovery of Powdery Mildew Resistance Gene Candidates From Aegilops Biuncialis Chromosome 2Mb Based On Transcriptome Sequencing." PloS One, vol. 14, no. 11, 2019, pp. e0220089.

Li H, Dong Z, Ma C, et al. Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing. PLoS One. 2019;14(11):e0220089.

Li, H., Dong, Z., Ma, C., Tian, X., Xiang, Z., Xia, Q., Ma, P., & Liu, W. (2019). Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing. PloS One, 14(11), e0220089. https://doi.org/10.1371/journal.pone.0220089

Li H, et al. Discovery of Powdery Mildew Resistance Gene Candidates From Aegilops Biuncialis Chromosome 2Mb Based On Transcriptome Sequencing. PLoS One. 2019;14(11):e0220089. PubMed PMID: 31710598.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing. AU - Li,Huanhuan, AU - Dong,Zhenjie, AU - Ma,Chao, AU - Tian,Xiubin, AU - Xiang,Zhiguo, AU - Xia,Qing, AU - Ma,Pengtao, AU - Liu,Wenxuan, Y1 - 2019/11/11/ PY - 2019/07/02/received PY - 2019/10/23/accepted PY - 2019/11/12/entrez PY - 2019/11/12/pubmed PY - 2020/3/20/medline SP - e0220089 EP - e0220089 JF - PloS one JO - PLoS One VL - 14 IS - 11 N2 - Powdery mildew is one of the most widespread diseases of wheat. The development and deployment of resistant varieties are one of the most economical and effective methods to manage this disease. Our previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred a high level of resistance to powdery mildew of wheat. In this study, resistance spectrum of TA7733 was assayed by using 15 Blumeria graminis f. sp. tritici (Bgt) isolates prevalent in different regions of China. The result indicated that TA7733 was highly resistant to all tested Bgt isolates and the gene(s) on chromosome 2Mb conferred broad-spectrum resistance to powdery mildew. In order to characterize mechanism of powdery mildew resistance by identifying candidates R-genes derived from Ae. biuncialis chromosome 2Mb and develop 2Mb-specific molecular markers, we performed RNA-seq analysis on TA7733 and CS. In total we identified 7,278 unigenes that showed specific expression in TA7733 pre and post Bgt-infection when compared to CS. Of these 7,278 unigenes, 295 were annotated as putative resistance (R) genes. Comparatively analysis of R-gene sequences from TA7733 and CS and integration CS Ref Seq v1.0 were used to develop R-gene specific primers. Of 295 R-genes we identified 53 R-genes were specific to 2Mb and could be involved in powdery mildew resistance. Functional annotation of majority of the 53 R-genes encoded nucleotide binding leucine rich repeat (NLR) protein. The broad-spectrum resistance to powdery mildew in TA7733 and availability of 2Mb-derived putative candidate R-gene specific molecular markers identified in this study will lay foundations for transferring powdery mildew resistance from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination. Our study also provides useful candidates for further isolation and cloning of powdery mildew resistance gene(s) from Ae. biuncialis chromosome 2Mb. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31710598/Discovery_of_powdery_mildew_resistance_gene_candidates_from_Aegilops_biuncialis_chromosome_2Mb_based_on_transcriptome_sequencing_ DB - PRIME DP - Unbound Medicine ER -