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Solvent and temperature effects of accelerated solvent extraction (ASE) coupled with ultra-high pressure liquid chromatography (UHPLC-DAD) technique for determination of thymoquinone in commercial food samples of black seeds (Nigella sativa).
Food Chem. 2020 Mar 30; 309:125740.FC

Abstract

Black seeds (Nigella sativa), is considered a traditional folk medicine in Saudi Arabia where it is widely available in the form of food supplements with limited information warranting its quality. This study aims to develop an effective and reliable method of black seeds evaluation and standardization in terms of Thymoquinone (THQ), obtained from various geographical sources i.e. Pakistan (PK), Saudi Arabia (SA) and India (I). Accelerated solvent extraction (ASE) was utilized for the first time to extract whereas Ultra High pressure liquid chromatography (UHPLC-DAD) was used to quantify THQ. Inductively coupled plasma mass spectrometry (ICP-MS) was used for elemental analysis of the samples. An ideal temperature (70 °C) and solvent (n-hexane) with extraction yield of 97.30% ± 3.98 was observed. In the case of commercial food samples an extraction yield and %recovery within a short time (42 ± 2 min) using least amount of solvent (49.5 ± 2 ml) was observed; SA (18.22 g; 91.1%) > PK (17.32 g; 86.6%) > I (16.33 g; 81.65%). UHPLC resulted a RT of 3.29 min for THQ with concentration (ng/ml) in the samples as; SA, 34410.36 > PK, 7778.95 > I, 4106.43. Elemental analysis revealed an order of SA > I > PK for tested elements. ASE resulted a high extract yield as compared to traditional methods of extraction whereas ASE-UHPLC/DAD showed a rapid and sensitive method of THQ quantification and quality determination in market available food samples for black seeds.

Authors+Show Affiliations

Natural Products and Alternative Medicines, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Saudi Arabia. Electronic address: rareiyadh@iau.edu.sa.Department of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Saudi Arabia; Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam, Kingdom of Saudi Arabia.Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Saudi Arabia.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

31711807

Citation

Ahmad, Rizwan, et al. "Solvent and Temperature Effects of Accelerated Solvent Extraction (ASE) Coupled With Ultra-high Pressure Liquid Chromatography (UHPLC-DAD) Technique for Determination of Thymoquinone in Commercial Food Samples of Black Seeds (Nigella Sativa)." Food Chemistry, vol. 309, 2020, p. 125740.
Ahmad R, Ahmad N, Shehzad A. Solvent and temperature effects of accelerated solvent extraction (ASE) coupled with ultra-high pressure liquid chromatography (UHPLC-DAD) technique for determination of thymoquinone in commercial food samples of black seeds (Nigella sativa). Food Chem. 2020;309:125740.
Ahmad, R., Ahmad, N., & Shehzad, A. (2020). Solvent and temperature effects of accelerated solvent extraction (ASE) coupled with ultra-high pressure liquid chromatography (UHPLC-DAD) technique for determination of thymoquinone in commercial food samples of black seeds (Nigella sativa). Food Chemistry, 309, 125740. https://doi.org/10.1016/j.foodchem.2019.125740
Ahmad R, Ahmad N, Shehzad A. Solvent and Temperature Effects of Accelerated Solvent Extraction (ASE) Coupled With Ultra-high Pressure Liquid Chromatography (UHPLC-DAD) Technique for Determination of Thymoquinone in Commercial Food Samples of Black Seeds (Nigella Sativa). Food Chem. 2020 Mar 30;309:125740. PubMed PMID: 31711807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Solvent and temperature effects of accelerated solvent extraction (ASE) coupled with ultra-high pressure liquid chromatography (UHPLC-DAD) technique for determination of thymoquinone in commercial food samples of black seeds (Nigella sativa). AU - Ahmad,Rizwan, AU - Ahmad,Niyaz, AU - Shehzad,Adeeb, Y1 - 2019/10/21/ PY - 2018/11/23/received PY - 2019/08/27/revised PY - 2019/10/17/accepted PY - 2019/11/13/pubmed PY - 2020/2/23/medline PY - 2019/11/13/entrez KW - Accelerated solvent extraction (ASE) KW - Black seed KW - ICP-MS KW - Thymoquinone KW - UHPLC-DAD SP - 125740 EP - 125740 JF - Food chemistry JO - Food Chem VL - 309 N2 - Black seeds (Nigella sativa), is considered a traditional folk medicine in Saudi Arabia where it is widely available in the form of food supplements with limited information warranting its quality. This study aims to develop an effective and reliable method of black seeds evaluation and standardization in terms of Thymoquinone (THQ), obtained from various geographical sources i.e. Pakistan (PK), Saudi Arabia (SA) and India (I). Accelerated solvent extraction (ASE) was utilized for the first time to extract whereas Ultra High pressure liquid chromatography (UHPLC-DAD) was used to quantify THQ. Inductively coupled plasma mass spectrometry (ICP-MS) was used for elemental analysis of the samples. An ideal temperature (70 °C) and solvent (n-hexane) with extraction yield of 97.30% ± 3.98 was observed. In the case of commercial food samples an extraction yield and %recovery within a short time (42 ± 2 min) using least amount of solvent (49.5 ± 2 ml) was observed; SA (18.22 g; 91.1%) > PK (17.32 g; 86.6%) > I (16.33 g; 81.65%). UHPLC resulted a RT of 3.29 min for THQ with concentration (ng/ml) in the samples as; SA, 34410.36 > PK, 7778.95 > I, 4106.43. Elemental analysis revealed an order of SA > I > PK for tested elements. ASE resulted a high extract yield as compared to traditional methods of extraction whereas ASE-UHPLC/DAD showed a rapid and sensitive method of THQ quantification and quality determination in market available food samples for black seeds. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/31711807/Solvent_and_temperature_effects_of_accelerated_solvent_extraction__ASE__coupled_with_ultra_high_pressure_liquid_chromatography__UHPLC_DAD__technique_for_determination_of_thymoquinone_in_commercial_food_samples_of_black_seeds__Nigella_sativa__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(19)31870-9 DB - PRIME DP - Unbound Medicine ER -