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Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island.
Sci Rep. 2019 11 14; 9(1):16860.SR

Abstract

The cytogenetic characteristics of the grasshopper Podisma sapporensis (two races 2n = 23♂ X0/XX and 2n = 22♂ neo-XY/neo-XX) were analysed through fluorescence in situ hybridization with rDNA and telomeric DNA probes, C-banding, fluorochrome and silver staining. For the first time, samples from the neighbourhood of a hybrid population (i.e., Mikuni Pass population) were studied. Our results indicated a significant degree of chromosomal differentiation between P. sapporensis races when comparing the number and position of the rDNA sites, as well as the heterochromatin composition and distribution obtained by C-banding and DAPI/CMA3 staining. Telomeric signals were usually detected at the distal and/or subdistal position of the autosomes; however, some chromosome ends lacked signals, probably due to a low number of telomeric repeats. On the other hand, telomeric DNA sequences were found as interstitial telomeric repeats in some autosomes, which can trigger a variety of genome instability. B chromosomes were found in specimens belonging to both main races from nine out of 22 localities. Four types of X chromosomes in the X0/XX race were identified. It was concluded that the physical mapping of rDNA sequences and heterochromatin are useful as additional markers for understanding the phylogeographic patterns of cytogenetic differentiation in P. sapporensis populations.

Authors+Show Affiliations

Department of Ecology and Environmental Sciences, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, 9030213, Japan. grzywacz@isez.pan.krakow.pl. Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016, Krakow, Poland. grzywacz@isez.pan.krakow.pl.Department of Ecology and Environmental Sciences, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, 9030213, Japan. htatsuta@agr.u-ryukyu.ac.jp. The United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima, 890-8580, Japan. htatsuta@agr.u-ryukyu.ac.jp.Institute of Systematics and Ecology of Animals, Russian Academy of Sciences, Siberian Branch, Frunze str. 11, 630091, Novosibirsk, Russia. Novosibirsk State University, Department of Natural Sciences, Pirogov str. 2, 630090, Novosibirsk, Russia.Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016, Krakow, Poland.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31728044

Citation

Grzywacz, Beata, et al. "Cytogenetic Markers Reveal a Reinforcement of Variation in the Tension Zone Between Chromosome Races in the Brachypterous Grasshopper Podisma Sapporensis Shir. On Hokkaido Island." Scientific Reports, vol. 9, no. 1, 2019, p. 16860.
Grzywacz B, Tatsuta H, Bugrov AG, et al. Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island. Sci Rep. 2019;9(1):16860.
Grzywacz, B., Tatsuta, H., Bugrov, A. G., & Warchałowska-Śliwa, E. (2019). Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island. Scientific Reports, 9(1), 16860. https://doi.org/10.1038/s41598-019-53416-7
Grzywacz B, et al. Cytogenetic Markers Reveal a Reinforcement of Variation in the Tension Zone Between Chromosome Races in the Brachypterous Grasshopper Podisma Sapporensis Shir. On Hokkaido Island. Sci Rep. 2019 11 14;9(1):16860. PubMed PMID: 31728044.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytogenetic markers reveal a reinforcement of variation in the tension zone between chromosome races in the brachypterous grasshopper Podisma sapporensis Shir. on Hokkaido Island. AU - Grzywacz,Beata, AU - Tatsuta,Haruki, AU - Bugrov,Alexander G, AU - Warchałowska-Śliwa,Elżbieta, Y1 - 2019/11/14/ PY - 2019/07/31/received PY - 2019/10/31/accepted PY - 2019/11/16/entrez PY - 2019/11/16/pubmed PY - 2020/10/31/medline SP - 16860 EP - 16860 JF - Scientific reports JO - Sci Rep VL - 9 IS - 1 N2 - The cytogenetic characteristics of the grasshopper Podisma sapporensis (two races 2n = 23♂ X0/XX and 2n = 22♂ neo-XY/neo-XX) were analysed through fluorescence in situ hybridization with rDNA and telomeric DNA probes, C-banding, fluorochrome and silver staining. For the first time, samples from the neighbourhood of a hybrid population (i.e., Mikuni Pass population) were studied. Our results indicated a significant degree of chromosomal differentiation between P. sapporensis races when comparing the number and position of the rDNA sites, as well as the heterochromatin composition and distribution obtained by C-banding and DAPI/CMA3 staining. Telomeric signals were usually detected at the distal and/or subdistal position of the autosomes; however, some chromosome ends lacked signals, probably due to a low number of telomeric repeats. On the other hand, telomeric DNA sequences were found as interstitial telomeric repeats in some autosomes, which can trigger a variety of genome instability. B chromosomes were found in specimens belonging to both main races from nine out of 22 localities. Four types of X chromosomes in the X0/XX race were identified. It was concluded that the physical mapping of rDNA sequences and heterochromatin are useful as additional markers for understanding the phylogeographic patterns of cytogenetic differentiation in P. sapporensis populations. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/31728044/Cytogenetic_markers_reveal_a_reinforcement_of_variation_in_the_tension_zone_between_chromosome_races_in_the_brachypterous_grasshopper_Podisma_sapporensis_Shir__on_Hokkaido_Island_ L2 - https://doi.org/10.1038/s41598-019-53416-7 DB - PRIME DP - Unbound Medicine ER -