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In vitro antibacterial activity of the manuka essential oil from Leptospermum scoparium combined with Tris-EDTA against Gram-negative bacterial isolates from dogs with otitis externa.
Vet Dermatol. 2020 Apr; 31(2):81-85.VD

Abstract

BACKGROUND

The increasing prevalence of antimicrobial resistance among bacteria in dogs with otitis externa has led to a need for novel therapeutic agents.

HYPOTHESIS/OBJECTIVE

To examine the antibacterial effects of manuka oil combined with ethylenediaminetetraacetic acid-tromethamine (Tris-EDTA) against Gram-negative bacteria isolates from dogs with otitis externa.

METHODS AND MATERIALS

A total of 53 clinical isolates including Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae ssp. pneumoniae and Proteus mirabilis. Antimicrobial susceptibility was determined using disk diffusion; the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of manuka essential oil, with or without Tris-EDTA, were investigated.

RESULTS

A total of 44 isolates were resistant to at least one antibiotic and 19 strains were multidrug-resistant, with resistance to at least one agent in three or more antimicrobial classes. The MICs and MBCs of manuka oil alone were ≥1% (v/v) and ≥2% (v/v), respectively. There was no antimicrobial effect of Tris-EDTA (1.125:0.3 mg/mL) without manuka oil. However, the combination of manuka oil with Tris-EDTA significantly decreased the MICs (ranging from 0.06% to 0.5%, v/v; P < 0.001) and MBCs (ranging from 0.06% to 1%, v/v; P < 0.001). There also was no significant difference between multidrug-resistant and nonresistant bacterial isolates in terms of the antimicrobial activity of manuka oil with Tris-EDTA.

CONCLUSIONS AND CLINICAL IMPORTANCE

The study findings suggest that manuka oil, especially when combined with Tris-EDTA, may be a promising alternative therapeutic option for Gram-negative otic pathogens. Clinical studies are needed to assess potential for in vivo ototoxic effects and efficacy.

Authors+Show Affiliations

Laboratory of Veterinary Dermatology, The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.Laboratory of Veterinary Dermatology, The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.Laboratory of Veterinary Dermatology, The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.Laboratory of Veterinary Dermatology, The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31729809

Citation

Song, Soon-Young, et al. "In Vitro Antibacterial Activity of the Manuka Essential Oil From Leptospermum Scoparium Combined With Tris-EDTA Against Gram-negative Bacterial Isolates From Dogs With Otitis Externa." Veterinary Dermatology, vol. 31, no. 2, 2020, pp. 81-85.
Song SY, Hyun JE, Kang JH, et al. In vitro antibacterial activity of the manuka essential oil from Leptospermum scoparium combined with Tris-EDTA against Gram-negative bacterial isolates from dogs with otitis externa. Vet Dermatol. 2020;31(2):81-85.
Song, S. Y., Hyun, J. E., Kang, J. H., & Hwang, C. Y. (2020). In vitro antibacterial activity of the manuka essential oil from Leptospermum scoparium combined with Tris-EDTA against Gram-negative bacterial isolates from dogs with otitis externa. Veterinary Dermatology, 31(2), 81-85. https://doi.org/10.1111/vde.12807
Song SY, et al. In Vitro Antibacterial Activity of the Manuka Essential Oil From Leptospermum Scoparium Combined With Tris-EDTA Against Gram-negative Bacterial Isolates From Dogs With Otitis Externa. Vet Dermatol. 2020;31(2):81-85. PubMed PMID: 31729809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antibacterial activity of the manuka essential oil from Leptospermum scoparium combined with Tris-EDTA against Gram-negative bacterial isolates from dogs with otitis externa. AU - Song,Soon-Young, AU - Hyun,Jae-Eun, AU - Kang,Jung-Hun, AU - Hwang,Cheol-Yong, Y1 - 2019/11/15/ PY - 2019/09/25/accepted PY - 2019/11/16/pubmed PY - 2019/11/16/medline PY - 2019/11/16/entrez SP - 81 EP - 85 JF - Veterinary dermatology JO - Vet Dermatol VL - 31 IS - 2 N2 - BACKGROUND: The increasing prevalence of antimicrobial resistance among bacteria in dogs with otitis externa has led to a need for novel therapeutic agents. HYPOTHESIS/OBJECTIVE: To examine the antibacterial effects of manuka oil combined with ethylenediaminetetraacetic acid-tromethamine (Tris-EDTA) against Gram-negative bacteria isolates from dogs with otitis externa. METHODS AND MATERIALS: A total of 53 clinical isolates including Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae ssp. pneumoniae and Proteus mirabilis. Antimicrobial susceptibility was determined using disk diffusion; the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of manuka essential oil, with or without Tris-EDTA, were investigated. RESULTS: A total of 44 isolates were resistant to at least one antibiotic and 19 strains were multidrug-resistant, with resistance to at least one agent in three or more antimicrobial classes. The MICs and MBCs of manuka oil alone were ≥1% (v/v) and ≥2% (v/v), respectively. There was no antimicrobial effect of Tris-EDTA (1.125:0.3 mg/mL) without manuka oil. However, the combination of manuka oil with Tris-EDTA significantly decreased the MICs (ranging from 0.06% to 0.5%, v/v; P < 0.001) and MBCs (ranging from 0.06% to 1%, v/v; P < 0.001). There also was no significant difference between multidrug-resistant and nonresistant bacterial isolates in terms of the antimicrobial activity of manuka oil with Tris-EDTA. CONCLUSIONS AND CLINICAL IMPORTANCE: The study findings suggest that manuka oil, especially when combined with Tris-EDTA, may be a promising alternative therapeutic option for Gram-negative otic pathogens. Clinical studies are needed to assess potential for in vivo ototoxic effects and efficacy. SN - 1365-3164 UR - https://www.unboundmedicine.com/medline/citation/31729809/In_vitro_antibacterial_activity_of_the_manuka_essential_oil_from_Leptospermum_scoparium_combined_with_Tris_EDTA_against_Gram_negative_bacterial_isolates_from_dogs_with_otitis_externa_ L2 - https://doi.org/10.1111/vde.12807 DB - PRIME DP - Unbound Medicine ER -