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Specialized Functions of Olive FAD2 Gene Family Members Related to Fruit Development and the Abiotic Stress Response.
Plant Cell Physiol. 2020 Feb 01; 61(2):427-441.PC

Abstract

Three different cDNA sequences, designated OepFAD2-3, OepFAD2-4 and OepFAD2-5, encoding three microsomal oleate desaturases (FAD2) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis and functional expression in yeast of the corresponding cDNAs confirm that they encode microsomal oleate desaturases. Gene expression and lipid analysis indicate that these three genes are not involved in the linoleic acid present in seed lipids, while OeFAD2-5, together with OeFAD2-2, contributes mostly to the linoleic acid present in the mesocarp and, therefore, in the olive oil. Our results have also shown that olive FAD2-3, FAD2-4 and FAD2-5 gene expression is not only spatially and temporally regulated in olive fruit, but also is cultivar-dependent, as well as regulated by water regime, temperature, light and wounding. All these data suggest specialized physiological roles for the olive FAD2 gene family members with respect to both aspects of the biosynthesis of the linoleic acid, either present in storage lipids that constitute the olive oil or being part of membrane lipids, which are involved in the response to abiotic stresses, and highlight the differences on FAD2 gene regulation between oilseeds and oil fruits.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (IG-CSIC), Campus Universidad Pablo de Olavide, Building 46, Ctra. Utrera Km.1, Sevilla 41013, Spain.Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (IG-CSIC), Campus Universidad Pablo de Olavide, Building 46, Ctra. Utrera Km.1, Sevilla 41013, Spain.Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (IG-CSIC), Campus Universidad Pablo de Olavide, Building 46, Ctra. Utrera Km.1, Sevilla 41013, Spain.Department of Biochemistry and Molecular Biology of Plant Products, Instituto de la Grasa (IG-CSIC), Campus Universidad Pablo de Olavide, Building 46, Ctra. Utrera Km.1, Sevilla 41013, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31730170

Citation

Luisa Hernández, M, et al. "Specialized Functions of Olive FAD2 Gene Family Members Related to Fruit Development and the Abiotic Stress Response." Plant & Cell Physiology, vol. 61, no. 2, 2020, pp. 427-441.
Luisa Hernández M, Dolores Sicardo M, Arjona PM, et al. Specialized Functions of Olive FAD2 Gene Family Members Related to Fruit Development and the Abiotic Stress Response. Plant Cell Physiol. 2020;61(2):427-441.
Luisa Hernández, M., Dolores Sicardo, M., Arjona, P. M., & Martínez-Rivas, J. M. (2020). Specialized Functions of Olive FAD2 Gene Family Members Related to Fruit Development and the Abiotic Stress Response. Plant & Cell Physiology, 61(2), 427-441. https://doi.org/10.1093/pcp/pcz208
Luisa Hernández M, et al. Specialized Functions of Olive FAD2 Gene Family Members Related to Fruit Development and the Abiotic Stress Response. Plant Cell Physiol. 2020 Feb 1;61(2):427-441. PubMed PMID: 31730170.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specialized Functions of Olive FAD2 Gene Family Members Related to Fruit Development and the Abiotic Stress Response. AU - Luisa Hernández,M, AU - Dolores Sicardo,M, AU - Arjona,Patricia M, AU - Martínez-Rivas,José M, PY - 2019/09/10/received PY - 2019/10/31/accepted PY - 2019/11/16/pubmed PY - 2020/10/21/medline PY - 2019/11/16/entrez KW - Abiotic stresses KW - FAD2 KW - Gene expression KW - Linoleic acid KW - Olea europaea KW - Olive SP - 427 EP - 441 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 61 IS - 2 N2 - Three different cDNA sequences, designated OepFAD2-3, OepFAD2-4 and OepFAD2-5, encoding three microsomal oleate desaturases (FAD2) have been isolated from olive (Olea europaea cv. Picual). Sequence analysis and functional expression in yeast of the corresponding cDNAs confirm that they encode microsomal oleate desaturases. Gene expression and lipid analysis indicate that these three genes are not involved in the linoleic acid present in seed lipids, while OeFAD2-5, together with OeFAD2-2, contributes mostly to the linoleic acid present in the mesocarp and, therefore, in the olive oil. Our results have also shown that olive FAD2-3, FAD2-4 and FAD2-5 gene expression is not only spatially and temporally regulated in olive fruit, but also is cultivar-dependent, as well as regulated by water regime, temperature, light and wounding. All these data suggest specialized physiological roles for the olive FAD2 gene family members with respect to both aspects of the biosynthesis of the linoleic acid, either present in storage lipids that constitute the olive oil or being part of membrane lipids, which are involved in the response to abiotic stresses, and highlight the differences on FAD2 gene regulation between oilseeds and oil fruits. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/31730170/Specialized_Functions_of_Olive_FAD2_Gene_Family_Members_Related_to_Fruit_Development_and_the_Abiotic_Stress_Response_ DB - PRIME DP - Unbound Medicine ER -