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Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans.
BMC Complement Altern Med. 2019 Nov 15; 19(1):313.BC

Abstract

BACKGROUND

Jianpi-yangwei (JPYW), a traditional Chinese medicine (TCM), helps to nourish the stomach and spleen and is primarily used to treat functional declines related to aging. This study aimed to explore the antiaging effects and mechanism of JPYW by employing a Caenorhabditis elegans model.

METHODS

Wild-type C. elegans N2 worms were cultured in growth medium with or without JPYW, and lifespan analysis, oxidative and heat stress resistance assays, and other aging-related assays were performed. The effects of JPYW on the levels of superoxide dismutase (SOD) and the expression of specific genes were examined to explore the underlying mechanism of JPYW.

RESULTS

Compared to control worms, JPYW-treated wild-type worms showed increased survival times under both normal and stress conditions (P < 0.05). JPYW-treated worms also exhibited enhanced reproduction, movement and growth and decreased intestinal lipofuscin accumulation compared to controls (P < 0.05). Furthermore, increased activity of SOD, downregulated expression levels of the proaging gene clk-2 and upregulated expression levels of the antiaging genes daf-16, skn-1, and sir-2.1 were observed in the JPYW group compared to the control group.

CONCLUSION

Our findings suggest that JPYW extends the lifespan of C. elegans and exerts antiaging effects by increasing the activity of an antioxidant enzyme (SOD) and by regulating the expression of aging-related genes. This study not only indicates that this Chinese compound exerts antiaging effects by activating and repressing target genes but also provides a proven methodology for studying the biological mechanisms of TCMs.

Authors+Show Affiliations

The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Da De Rd., Yuexiu District, Guangzhou, Guangdong Province, People's Republic of China, 510120. yangyo@vip.tom.com.The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Da De Rd., Yuexiu District, Guangzhou, Guangdong Province, People's Republic of China, 510120.The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Da De Rd., Yuexiu District, Guangzhou, Guangdong Province, People's Republic of China, 510120. xufuping163@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31730453

Citation

Zeng, Liling, et al. "Antiaging Effect of a Jianpi-yangwei Formula in Caenorhabditis Elegans." BMC Complementary and Alternative Medicine, vol. 19, no. 1, 2019, p. 313.
Zeng L, Yang Z, Yun T, et al. Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans. BMC Complement Altern Med. 2019;19(1):313.
Zeng, L., Yang, Z., Yun, T., Fan, S., Pei, Z., Chen, Z., Sun, C., & Xu, F. (2019). Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans. BMC Complementary and Alternative Medicine, 19(1), 313. https://doi.org/10.1186/s12906-019-2704-4
Zeng L, et al. Antiaging Effect of a Jianpi-yangwei Formula in Caenorhabditis Elegans. BMC Complement Altern Med. 2019 Nov 15;19(1):313. PubMed PMID: 31730453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans. AU - Zeng,Liling, AU - Yang,Zhimin, AU - Yun,Tianchan, AU - Fan,Shaoyi, AU - Pei,Zhong, AU - Chen,Ziwen, AU - Sun,Chen, AU - Xu,Fuping, Y1 - 2019/11/15/ PY - 2019/03/10/received PY - 2019/10/09/accepted PY - 2019/11/16/entrez PY - 2019/11/16/pubmed PY - 2020/1/29/medline KW - Aging KW - Caenorhabditis elegans KW - Jianpi-yangwei formula KW - Traditional Chinese medicine SP - 313 EP - 313 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 19 IS - 1 N2 - BACKGROUND: Jianpi-yangwei (JPYW), a traditional Chinese medicine (TCM), helps to nourish the stomach and spleen and is primarily used to treat functional declines related to aging. This study aimed to explore the antiaging effects and mechanism of JPYW by employing a Caenorhabditis elegans model. METHODS: Wild-type C. elegans N2 worms were cultured in growth medium with or without JPYW, and lifespan analysis, oxidative and heat stress resistance assays, and other aging-related assays were performed. The effects of JPYW on the levels of superoxide dismutase (SOD) and the expression of specific genes were examined to explore the underlying mechanism of JPYW. RESULTS: Compared to control worms, JPYW-treated wild-type worms showed increased survival times under both normal and stress conditions (P < 0.05). JPYW-treated worms also exhibited enhanced reproduction, movement and growth and decreased intestinal lipofuscin accumulation compared to controls (P < 0.05). Furthermore, increased activity of SOD, downregulated expression levels of the proaging gene clk-2 and upregulated expression levels of the antiaging genes daf-16, skn-1, and sir-2.1 were observed in the JPYW group compared to the control group. CONCLUSION: Our findings suggest that JPYW extends the lifespan of C. elegans and exerts antiaging effects by increasing the activity of an antioxidant enzyme (SOD) and by regulating the expression of aging-related genes. This study not only indicates that this Chinese compound exerts antiaging effects by activating and repressing target genes but also provides a proven methodology for studying the biological mechanisms of TCMs. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/31730453/Antiaging_effect_of_a_Jianpi_yangwei_formula_in_Caenorhabditis_elegans_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-019-2704-4 DB - PRIME DP - Unbound Medicine ER -