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Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis.
J Hazard Mater 2020; 385:121531JH

Abstract

Fipronil has been widely used in agriculture to prevent aggressive insects from damaging agricultural products. Fipronil residues circulate in the environment and they have been detected in non-targeted organisms in aquatic environments. To study the effect of fipronil toxicity on environmental health, 6 h post fertilization (hpf) zebrafish embryos were treated with fipronil for 72 h. LC50 value was obtained by applying varying concentrations of fipronil to zebrafish embryos for 72 h. As zebrafish embryos are useful vertebrate models for studying developmental and genetic findings in toxicology research, they were exposed to fipronil to study detailed elucidating mechanisms with hazardous end points of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, such as increased mortality, shortened body length, and reduced hatchability. Furthermore, observed heart defects, including edema and irregular heartbeat were caused due to abnormal blood circulation. In transgenic zebrafish models (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations were indicated by eGFP+ endothelial cells. Moreover, neurogenic defects were observed by studying dsRED+ motor neurons and oligodendrocytes. This study demonstrates fipronil accumulation in aquatic environment and its ability to impair essential processes, such as angiogenesis and neurogenesis during early developmental stage of zebrafish, along with general developmental toxicity.

Authors+Show Affiliations

Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address: ghsong@korea.ac.kr.Department of Food and Nutrition, Kookmin University, Seoul, 02707, Republic of Korea. Electronic address: wlim@kookmin.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31732348

Citation

Park, Hahyun, et al. "Developmental Toxicity of Fipronil in Early Development of Zebrafish (Danio Rerio) Larvae: Disrupted Vascular Formation With Angiogenic Failure and Inhibited Neurogenesis." Journal of Hazardous Materials, vol. 385, 2020, p. 121531.
Park H, Lee JY, Park S, et al. Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis. J Hazard Mater. 2020;385:121531.
Park, H., Lee, J. Y., Park, S., Song, G., & Lim, W. (2020). Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis. Journal of Hazardous Materials, 385, p. 121531. doi:10.1016/j.jhazmat.2019.121531.
Park H, et al. Developmental Toxicity of Fipronil in Early Development of Zebrafish (Danio Rerio) Larvae: Disrupted Vascular Formation With Angiogenic Failure and Inhibited Neurogenesis. J Hazard Mater. 2020 Mar 5;385:121531. PubMed PMID: 31732348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis. AU - Park,Hahyun, AU - Lee,Jin-Young, AU - Park,Sunwoo, AU - Song,Gwonhwa, AU - Lim,Whasun, Y1 - 2019/10/30/ PY - 2019/08/22/received PY - 2019/10/22/revised PY - 2019/10/22/accepted PY - 2019/11/17/pubmed PY - 2019/11/17/medline PY - 2019/11/17/entrez KW - Cardiac failure KW - Developmental toxicity KW - Embryogenesis KW - Fipronil KW - Neuronal defect SP - 121531 EP - 121531 JF - Journal of hazardous materials JO - J. Hazard. Mater. VL - 385 N2 - Fipronil has been widely used in agriculture to prevent aggressive insects from damaging agricultural products. Fipronil residues circulate in the environment and they have been detected in non-targeted organisms in aquatic environments. To study the effect of fipronil toxicity on environmental health, 6 h post fertilization (hpf) zebrafish embryos were treated with fipronil for 72 h. LC50 value was obtained by applying varying concentrations of fipronil to zebrafish embryos for 72 h. As zebrafish embryos are useful vertebrate models for studying developmental and genetic findings in toxicology research, they were exposed to fipronil to study detailed elucidating mechanisms with hazardous end points of toxicity. Cell cycle arrest-related apoptosis supported pathological alterations, such as increased mortality, shortened body length, and reduced hatchability. Furthermore, observed heart defects, including edema and irregular heartbeat were caused due to abnormal blood circulation. In transgenic zebrafish models (fli1:eGFP and olig2:dsRED), disrupted blood vessel formations were indicated by eGFP+ endothelial cells. Moreover, neurogenic defects were observed by studying dsRED+ motor neurons and oligodendrocytes. This study demonstrates fipronil accumulation in aquatic environment and its ability to impair essential processes, such as angiogenesis and neurogenesis during early developmental stage of zebrafish, along with general developmental toxicity. SN - 1873-3336 UR - https://www.unboundmedicine.com/medline/citation/31732348/Developmental_toxicity_of_fipronil_in_early_development_of_zebrafish__Danio_rerio__larvae:_Disrupted_vascular_formation_with_angiogenic_failure_and_inhibited_neurogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(19)31485-2 DB - PRIME DP - Unbound Medicine ER -