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Effects of subchronic exposure of mercuric chloride on intestinal histology and microbiota in the cecum of chicken.
Ecotoxicol Environ Saf 2020; 188:109920EE

Abstract

This study aimed to investigate the influences of mercuric chloride (HgCl2, 250 ppm, drink water) on the growth performance, cecal morphology and microbiota of chickens (n = 60) after 30, 60, and 90 days of exposure. A control group of sixty chickens received water free of HgCl2. Our results suggested that mercury exposure reduced the body weight and changed the cecal morphology of chickens after the 90-day treatment. Furthermore, sequence analysis of 16 S rRNA gene revealed that the diversity and composition of cecal microbiota in chickens differed between the control and exposure group. At the phylum level, Proteobacteria and Tenericutes phyla both significantly increased in mercury exposure groups on day 30 while only Tenericutes phyla significantly increased on day 60. At the genus level, we observed that the change in microbial populations are most dramatic on day 30. Besides, compared with the control group, the genus Prevotellaceae_UCG-001 significantly increased in exposure group on day 30 but showed no significant difference on day 60, whereas there was a significant decrease on day 90. PICRUSt analysis revealed potential metabolic changes, such as Bacterial invasion of epithelial cells and Metabolism of xenobiotics, associated with mercury exposure in chickens. Taken together, the data show that subchronic exposure to mercury not only affected the growth and development but also caused the dysbiosis of gut microbiota, which may further induced metabolic disorders in chickens.

Authors+Show Affiliations

Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.Animal Husbandry and Veterinary Department of Ganzhou, Jiangxi, China.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China. Electronic address: Pingliujx@163.com.Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, China. Electronic address: xqguo20720@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31733937

Citation

Zhou, Changming, et al. "Effects of Subchronic Exposure of Mercuric Chloride On Intestinal Histology and Microbiota in the Cecum of Chicken." Ecotoxicology and Environmental Safety, vol. 188, 2020, p. 109920.
Zhou C, Xu P, Huang C, et al. Effects of subchronic exposure of mercuric chloride on intestinal histology and microbiota in the cecum of chicken. Ecotoxicol Environ Saf. 2020;188:109920.
Zhou, C., Xu, P., Huang, C., Liu, G., Chen, S., Hu, G., ... Guo, X. (2020). Effects of subchronic exposure of mercuric chloride on intestinal histology and microbiota in the cecum of chicken. Ecotoxicology and Environmental Safety, 188, p. 109920. doi:10.1016/j.ecoenv.2019.109920.
Zhou C, et al. Effects of Subchronic Exposure of Mercuric Chloride On Intestinal Histology and Microbiota in the Cecum of Chicken. Ecotoxicol Environ Saf. 2020 Jan 30;188:109920. PubMed PMID: 31733937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of subchronic exposure of mercuric chloride on intestinal histology and microbiota in the cecum of chicken. AU - Zhou,Changming, AU - Xu,Puzhi, AU - Huang,Cheng, AU - Liu,Guohui, AU - Chen,Shupeng, AU - Hu,Guoliang, AU - Li,Guyue, AU - Liu,Ping, AU - Guo,Xiaoquan, Y1 - 2019/11/13/ PY - 2019/09/15/received PY - 2019/10/31/revised PY - 2019/11/02/accepted PY - 2019/11/18/pubmed PY - 2019/11/18/medline PY - 2019/11/18/entrez KW - 16S rRNA KW - Chicken KW - Gut microbiota KW - Heavy metal pollution KW - Mercury KW - Metabolic disorders SP - 109920 EP - 109920 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 188 N2 - This study aimed to investigate the influences of mercuric chloride (HgCl2, 250 ppm, drink water) on the growth performance, cecal morphology and microbiota of chickens (n = 60) after 30, 60, and 90 days of exposure. A control group of sixty chickens received water free of HgCl2. Our results suggested that mercury exposure reduced the body weight and changed the cecal morphology of chickens after the 90-day treatment. Furthermore, sequence analysis of 16 S rRNA gene revealed that the diversity and composition of cecal microbiota in chickens differed between the control and exposure group. At the phylum level, Proteobacteria and Tenericutes phyla both significantly increased in mercury exposure groups on day 30 while only Tenericutes phyla significantly increased on day 60. At the genus level, we observed that the change in microbial populations are most dramatic on day 30. Besides, compared with the control group, the genus Prevotellaceae_UCG-001 significantly increased in exposure group on day 30 but showed no significant difference on day 60, whereas there was a significant decrease on day 90. PICRUSt analysis revealed potential metabolic changes, such as Bacterial invasion of epithelial cells and Metabolism of xenobiotics, associated with mercury exposure in chickens. Taken together, the data show that subchronic exposure to mercury not only affected the growth and development but also caused the dysbiosis of gut microbiota, which may further induced metabolic disorders in chickens. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/31733937/Effects_of_subchronic_exposure_of_mercuric_chloride_on_intestinal_histology_and_microbiota_in_the_cecum_of_chicken L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(19)31251-5 DB - PRIME DP - Unbound Medicine ER -