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Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter.
Radiat Environ Biophys. 2020 03; 59(1):131-144.RE

Abstract

Biological dosimetry based on sulfhemoglobin (SHb), methemoglobin (MetHb), and carboxyhemoglobin (HbCO) levels was evaluated. SHb, MetHb and HbCO levels were estimated in erythrocytes of mice irradiated by γ rays from a 60Co source using the method of multi-component spectrophotometric analysis developed recently. In this method, absorption measurements of diluted aqueous Hb-solution were made at λ = 500, 569, 577 and 620 nm, and using the mathematical formulas based on multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation, the concentrations of various Hb-derivatives and total Hb in mice blood were estimated. The dose range of γ rays was from 0.5 to 8 Gy and the dose rate was 0.5 Gy min-1. Among all Hb-derivatives, MetHb, SHb and HbCO demonstrated an unambiguous dose-dependent response. For SHb and MetHb, the detection limits were about 0.5 Gy and 1 Gy, respectively. After irradiation, high levels of MetHb, SHb and HbCO persisted for at least 10 days, and the maximal increase of MetHb, SHb and HbCO occurred up to 24 h following γ irradiation. The use of this "MetHb + SHb + HbCO"-derivatives-based absorbed dose relationship showed a high accuracy. It is concluded that simultaneous determination of MetHb, SHb and HbCO, by multi-component spectrophotometry provides a quick, simple, sensitive, accurate, stable and inexpensive biological indicator for the early assessment of the absorbed dose in mice.

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Authors+Show Affiliations

Attia AMM
Genetic Engineering and Biotechnology Division, Biochemistry Department, National Research Centre, Dokki, Giza, Egypt.
Aboulthana WM
Genetic Engineering and Biotechnology Division, Biochemistry Department, National Research Centre, Dokki, Giza, Egypt.
Hassan GM
Division of Thermometry and Ionizing Radiation Metrology, Department of Ionizing Radiation Metrology, National Institute of Standards, Giza, Egypt. eslam_genius@hotmail.com.
Aboelezz E
Division of Thermometry and Ionizing Radiation Metrology, Department of Ionizing Radiation Metrology, National Institute of Standards, Giza, Egypt.

MeSH

AnimalsBiomarkersCarboxyhemoglobinErythrocytesGamma RaysIn Vivo DosimetryMaleMethemoglobinMiceSulfhemoglobinWhole-Body Irradiation

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31734721

Citation

Attia, A M M., et al. "Assessment of Absorbed Dose of Gamma Rays Using the Simultaneous Determination of Inactive Hemoglobin Derivatives as a Biological Dosimeter." Radiation and Environmental Biophysics, vol. 59, no. 1, 2020, pp. 131-144.
Attia AMM, Aboulthana WM, Hassan GM, et al. Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter. Radiat Environ Biophys. 2020;59(1):131-144.
Attia, A. M. M., Aboulthana, W. M., Hassan, G. M., & Aboelezz, E. (2020). Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter. Radiation and Environmental Biophysics, 59(1), 131-144. https://doi.org/10.1007/s00411-019-00821-1
Attia AMM, et al. Assessment of Absorbed Dose of Gamma Rays Using the Simultaneous Determination of Inactive Hemoglobin Derivatives as a Biological Dosimeter. Radiat Environ Biophys. 2020;59(1):131-144. PubMed PMID: 31734721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter. AU - Attia,A M M, AU - Aboulthana,W M, AU - Hassan,G M, AU - Aboelezz,E, Y1 - 2019/11/16/ PY - 2019/01/05/received PY - 2019/11/03/accepted PY - 2019/11/18/pubmed PY - 2021/2/9/medline PY - 2019/11/18/entrez KW - Biological dosimetry KW - Carboxyhemoglobin (HbCO) KW - Methemoglobin (MetHb) KW - Oxyhemoglobin (HbO2) KW - Sulfhemoglobin (SHb) KW - γ-Irradiation SP - 131 EP - 144 JF - Radiation and environmental biophysics JO - Radiat Environ Biophys VL - 59 IS - 1 N2 - Biological dosimetry based on sulfhemoglobin (SHb), methemoglobin (MetHb), and carboxyhemoglobin (HbCO) levels was evaluated. SHb, MetHb and HbCO levels were estimated in erythrocytes of mice irradiated by γ rays from a 60Co source using the method of multi-component spectrophotometric analysis developed recently. In this method, absorption measurements of diluted aqueous Hb-solution were made at λ = 500, 569, 577 and 620 nm, and using the mathematical formulas based on multi-component spectrophotometric analysis and the mathematical Gaussian elimination method for matrix calculation, the concentrations of various Hb-derivatives and total Hb in mice blood were estimated. The dose range of γ rays was from 0.5 to 8 Gy and the dose rate was 0.5 Gy min-1. Among all Hb-derivatives, MetHb, SHb and HbCO demonstrated an unambiguous dose-dependent response. For SHb and MetHb, the detection limits were about 0.5 Gy and 1 Gy, respectively. After irradiation, high levels of MetHb, SHb and HbCO persisted for at least 10 days, and the maximal increase of MetHb, SHb and HbCO occurred up to 24 h following γ irradiation. The use of this "MetHb + SHb + HbCO"-derivatives-based absorbed dose relationship showed a high accuracy. It is concluded that simultaneous determination of MetHb, SHb and HbCO, by multi-component spectrophotometry provides a quick, simple, sensitive, accurate, stable and inexpensive biological indicator for the early assessment of the absorbed dose in mice. SN - 1432-2099 UR - https://www.unboundmedicine.com/medline/citation/31734721/Assessment_of_absorbed_dose_of_gamma_rays_using_the_simultaneous_determination_of_inactive_hemoglobin_derivatives_as_a_biological_dosimeter_ DB - PRIME DP - Unbound Medicine ER -