Tags

Type your tag names separated by a space and hit enter

Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue.
Int J Biol Macromol. 2020 Mar 15; 147:1125-1135.IJ

Abstract

A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150. A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa. Thespecific rotation of AGP was detected by a polarimeter and it was +45°. Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54. The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-(→ backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches. The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP. The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular. The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure. Furthermore, the AGP exhibited certain inhibition activity on α-glucosidase.

Authors+Show Affiliations

Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China; Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 place Jussieu, 75005 Paris, France.Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China; Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 place Jussieu, 75005 Paris, France.Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China; Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 place Jussieu, 75005 Paris, France.Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China; Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 place Jussieu, 75005 Paris, France.Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, PR China; Université Pierre et Marie Curie-Paris 6, Institut Parisien de Chimie Moléculaire, UMR CNRS 8232, 4 place Jussieu, 75005 Paris, France. Electronic address: zhyuanzhu@tust.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31739069

Citation

Zhang, Xiaojing, et al. "Chemical Structure and Inhibition On Α-glucosidase of Polysaccharide With Alkaline-extracted From Glycyrrhiza Inflata Residue." International Journal of Biological Macromolecules, vol. 147, 2020, pp. 1125-1135.
Zhang X, Kong X, Hao Y, et al. Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue. Int J Biol Macromol. 2020;147:1125-1135.
Zhang, X., Kong, X., Hao, Y., Zhang, X., & Zhu, Z. (2020). Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue. International Journal of Biological Macromolecules, 147, 1125-1135. https://doi.org/10.1016/j.ijbiomac.2019.10.081
Zhang X, et al. Chemical Structure and Inhibition On Α-glucosidase of Polysaccharide With Alkaline-extracted From Glycyrrhiza Inflata Residue. Int J Biol Macromol. 2020 Mar 15;147:1125-1135. PubMed PMID: 31739069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue. AU - Zhang,Xiaojing, AU - Kong,Xiangru, AU - Hao,Yali, AU - Zhang,Xiaoling, AU - Zhu,Zhenyuan, Y1 - 2019/11/15/ PY - 2019/06/07/received PY - 2019/08/19/revised PY - 2019/10/08/accepted PY - 2019/11/19/pubmed PY - 2020/12/30/medline PY - 2019/11/19/entrez KW - Alkaline-extraction KW - Glycyrrhiza inflata residue KW - Polysaccharide SP - 1125 EP - 1135 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 147 N2 - A new neutral polysaccharide, named AGP, was extracted from glycyrrhiza residue by 5% NaOH alkaline solution and purified by DEAE-celluloseand Sephadex G-150. A single and symmetrical peak was shown by HPLC, indicating that AGP is a homogeneous polysaccharide with a molecular weight of 2.89 × 103 KDa. Thespecific rotation of AGP was detected by a polarimeter and it was +45°. Monosaccharide composition analysis indicated that AGP was consisted of l-rhamnose: l-arabinose: d-xylose: d-mannose: d-glucose and d-galactose with a molar ratio of 1:2.33:2.85:0.69:3.05:1.54. The structure of AGP was analyzed by GC-MS, periodate oxidation, Smith degradation, FT-IR, methylation and NMR, which indicated that the AGP was composed of → 6)-β-d-Glcp-(→ backbone and the →4)-α-d-Xylp-(1→, →5)-α-l-Araf-(1→, →3)-α-l-Rhap-(1→, →6)-α-d-Galp-(1→, →3,6)-α-Manp-(1→ and →1)-β-d-Glcp as branches. The results of Congo red experiment and circular dichroism (CD) showed that there was triple helix conformation in AGP. The micro-structure of AGP were detected by scanning electron microscopy (SEM), which concluded that the shape of AGP was a "thin slice" and its structure is not regular. The crystal configuration was identified by X-ray diffraction (XRD), showing that there is no crystal structure. Furthermore, the AGP exhibited certain inhibition activity on α-glucosidase. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/31739069/Chemical_structure_and_inhibition_on_α_glucosidase_of_polysaccharide_with_alkaline_extracted_from_glycyrrhiza_inflata_residue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(19)34270-9 DB - PRIME DP - Unbound Medicine ER -