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Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit.
Nucleic Acids Res. 2020 01 10; 48(1):405-420.NA

Abstract

More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many AFs, others remain poorly characterized. Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore, we identify binding sites for Pol5 in the 5' external transcribed spacer and within domain III of the 25S rRNA sequence. Consistent with this, we reveal that Pol5 is required for recruitment of ribosomal proteins that form the polypeptide exit tunnel in the LSU and that depletion of Pol5 impairs the release of 5' ETS fragments from early pre-40S particles. The dual functions of Pol5 in 60S assembly and recycling of pre-40S AFs suggest that this factor could contribute to ensuring the stoichiometric production of ribosomal subunits.

Authors+Show Affiliations

Department of Biochemistry III, University of Regensburg, Universitätstrasse 31, 93053 Regensburg, Germany.Department of Molecular Biology, University Medical Center Göttingen, Humboldtallee 23, 37073 Göttingen, Germany.Department of Biochemistry III, University of Regensburg, Universitätstrasse 31, 93053 Regensburg, Germany.Department of Molecular Biology, University Medical Center Göttingen, Humboldtallee 23, 37073 Göttingen, Germany. Göttingen Center for Molecular Biosciences, Georg-August University, Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.Department of Molecular Biology, University Medical Center Göttingen, Humboldtallee 23, 37073 Göttingen, Germany.Department of Biochemistry III, University of Regensburg, Universitätstrasse 31, 93053 Regensburg, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31745560

Citation

Braun, Christina M., et al. "Pol5 Is Required for Recycling of Small Subunit Biogenesis Factors and for Formation of the Peptide Exit Tunnel of the Large Ribosomal Subunit." Nucleic Acids Research, vol. 48, no. 1, 2020, pp. 405-420.
Braun CM, Hackert P, Schmid CE, et al. Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit. Nucleic Acids Res. 2020;48(1):405-420.
Braun, C. M., Hackert, P., Schmid, C. E., Bohnsack, M. T., Bohnsack, K. E., & Perez-Fernandez, J. (2020). Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit. Nucleic Acids Research, 48(1), 405-420. https://doi.org/10.1093/nar/gkz1079
Braun CM, et al. Pol5 Is Required for Recycling of Small Subunit Biogenesis Factors and for Formation of the Peptide Exit Tunnel of the Large Ribosomal Subunit. Nucleic Acids Res. 2020 01 10;48(1):405-420. PubMed PMID: 31745560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit. AU - Braun,Christina M, AU - Hackert,Philipp, AU - Schmid,Catharina E, AU - Bohnsack,Markus T, AU - Bohnsack,Katherine E, AU - Perez-Fernandez,Jorge, PY - 2019/11/04/accepted PY - 2019/10/29/revised PY - 2019/05/24/received PY - 2019/11/21/pubmed PY - 2020/3/18/medline PY - 2019/11/21/entrez SP - 405 EP - 420 JF - Nucleic acids research JO - Nucleic Acids Res. VL - 48 IS - 1 N2 - More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many AFs, others remain poorly characterized. Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore, we identify binding sites for Pol5 in the 5' external transcribed spacer and within domain III of the 25S rRNA sequence. Consistent with this, we reveal that Pol5 is required for recruitment of ribosomal proteins that form the polypeptide exit tunnel in the LSU and that depletion of Pol5 impairs the release of 5' ETS fragments from early pre-40S particles. The dual functions of Pol5 in 60S assembly and recycling of pre-40S AFs suggest that this factor could contribute to ensuring the stoichiometric production of ribosomal subunits. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/31745560/Pol5_is_required_for_recycling_of_small_subunit_biogenesis_factors_and_for_formation_of_the_peptide_exit_tunnel_of_the_large_ribosomal_subunit_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkz1079 DB - PRIME DP - Unbound Medicine ER -