Tags

Type your tag names separated by a space and hit enter

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae.
Genes (Basel). 2019 11 20; 10(12)G

Abstract

Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 602267 differentially expressed unigenes (DEUs). Among these DEUs, 31-790 unigenes were classified into 66-786 categories of gene ontology (GO) functional groups, and 24-760 DEUs could be mapped into 54-268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids.

Links

Authors+Show Affiliations

College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China. Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China.

Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, Anhui Province, China.

MeSH

AnimalsAphidsChlorpyrifosGene Expression ProfilingGenes, InsectInsecticide ResistanceInsecticidesNeonicotinoidsNitro CompoundsTranscriptome

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31757092

Citation

Wei, Ning, et al. "Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes After Exposure to Insecticide in Sitobion Avenae." Genes, vol. 10, no. 12, 2019.

Wei N, Zhong Y, Lin L, et al. Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae. Genes (Basel). 2019;10(12).

Wei, N., Zhong, Y., Lin, L., Xie, M., Zhang, G., Su, W., Li, C., & Chen, H. (2019). Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae. Genes, 10(12). https://doi.org/10.3390/genes10120951

Wei N, et al. Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes After Exposure to Insecticide in Sitobion Avenae. Genes (Basel). 2019 11 20;10(12) PubMed PMID: 31757092.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae. AU - Wei,Ning, AU - Zhong,Yongzhi, AU - Lin,Lulu, AU - Xie,Minghui, AU - Zhang,Guangling, AU - Su,Weihua, AU - Li,Chuanren, AU - Chen,Haoliang, Y1 - 2019/11/20/ PY - 2019/09/20/received PY - 2019/11/13/revised PY - 2019/11/18/accepted PY - 2019/11/24/entrez PY - 2019/11/24/pubmed PY - 2020/5/13/medline KW - RNA-seq KW - Sitobion avenae KW - differentially expressed unigenes KW - insecticide tolerance-related genes JF - Genes JO - Genes (Basel) VL - 10 IS - 12 N2 - Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 602267 differentially expressed unigenes (DEUs). Among these DEUs, 31-790 unigenes were classified into 66-786 categories of gene ontology (GO) functional groups, and 24-760 DEUs could be mapped into 54-268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids. SN - 2073-4425 UR - https://www.unboundmedicine.com/medline/citation/31757092/Transcriptome_Analysis_and_Identification_of_Insecticide_Tolerance_Related_Genes_after_Exposure_to_Insecticide_in_Sitobion_avenae_ DB - PRIME DP - Unbound Medicine ER -