Tags

Type your tag names separated by a space and hit enter

Ameliorating potency of Chenopodium album Linn. and vitamin C against mercuric chloride-induced oxidative stress in testes of Sprague Dawley rats.
Environ Health Prev Med 2019; 24(1):62EH

Abstract

BACKGROUND

Mercury has been documented as an industrial risk that posed a serious danger to human health. Mercury exposure results in oxidative stress that may lead to the pathogenesis of male reproductive dysfunction. The present study investigated the ameliorating potential of Chenopodium album L. and vitamin C against mercuric chloride-induced oxidative deterioration of reproductive functions in adult male rats.

METHODS

Group 1 (control) received saline. Group 2 received Mercury (0.15 mg/kg b.w, i.p) dissolved in distilled water. Groups 3 and 4 were given oral gavage of vitamin C (200 mg/kg b.w) and the ethanolic extract of C. album (200 mg/kg b.w) respectively, along with Mercury (0.15 mg/kg b.w, i.p). Group 5 was treated only with C. album (200 mg/kg b.w). After 30 days of the treatment, the rats were dissected and their testicular tissue and the cauda epididymis were used for biochemical analysis while blood plasma was used for protein determination.

RESULTS

The applied dose-treatment of Mercury-induced oxidative stress in the testis and cauda epididymis tissues of the rats was apparent by a noteworthy decrease in total protein, CAT, SOD, POD, and GST values while there was increase in ROS and TBARS levels. Furthermore, Mercury decreases daily sperm production and enhanced sperm DNA damage as noticeable by an increase in the head and tail length of comets and decrease in intact DNA. There was no significant effect on the body weight and the weight of the reproductive tissues. Treatment with C. album significantly ameliorated the total protein, ROS, and TBARS content. Similarly, the level of CAT, SOD, POD, and GST was significantly improved and the daily sperm production was significantly increased. Furthermore, C. album administration significantly protected Mercury-induced sperm DNA damage. The results of the extract treatment group were compared with those of vitamin C in detoxifying the oxidative stress and restoring the sperm parameters.

CONCLUSION

C. album showed protection against Mercury-induced oxidative stress by ameliorating antioxidant enzyme activity, daily sperm production, and DNA damage in rat testes. This suggests that C. album could be beneficial against toxicity induced by an environmental toxicant.

Authors+Show Affiliations

Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan. ruhail12345@yahoo.com. Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia. ruhail12345@yahoo.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31759394

Citation

Jahan, Sarwat, et al. "Ameliorating Potency of Chenopodium Album Linn. and Vitamin C Against Mercuric Chloride-induced Oxidative Stress in Testes of Sprague Dawley Rats." Environmental Health and Preventive Medicine, vol. 24, no. 1, 2019, p. 62.
Jahan S, Azad T, Ayub A, et al. Ameliorating potency of Chenopodium album Linn. and vitamin C against mercuric chloride-induced oxidative stress in testes of Sprague Dawley rats. Environ Health Prev Med. 2019;24(1):62.
Jahan, S., Azad, T., Ayub, A., Ullah, A., Afsar, T., Almajwal, A., & Razak, S. (2019). Ameliorating potency of Chenopodium album Linn. and vitamin C against mercuric chloride-induced oxidative stress in testes of Sprague Dawley rats. Environmental Health and Preventive Medicine, 24(1), p. 62. doi:10.1186/s12199-019-0820-x.
Jahan S, et al. Ameliorating Potency of Chenopodium Album Linn. and Vitamin C Against Mercuric Chloride-induced Oxidative Stress in Testes of Sprague Dawley Rats. Environ Health Prev Med. 2019 Nov 23;24(1):62. PubMed PMID: 31759394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorating potency of Chenopodium album Linn. and vitamin C against mercuric chloride-induced oxidative stress in testes of Sprague Dawley rats. AU - Jahan,Sarwat, AU - Azad,Tayyaba, AU - Ayub,Amina, AU - Ullah,Asad, AU - Afsar,Tayyaba, AU - Almajwal,Ali, AU - Razak,Suhail, Y1 - 2019/11/23/ PY - 2019/06/01/received PY - 2019/09/23/accepted PY - 2019/11/25/entrez PY - 2019/11/25/pubmed PY - 2019/12/21/medline KW - Chenopodium album Linn KW - Comet assay KW - Mercuric chloride KW - Oxidative stress KW - Testes KW - Vitamin C SP - 62 EP - 62 JF - Environmental health and preventive medicine JO - Environ Health Prev Med VL - 24 IS - 1 N2 - BACKGROUND: Mercury has been documented as an industrial risk that posed a serious danger to human health. Mercury exposure results in oxidative stress that may lead to the pathogenesis of male reproductive dysfunction. The present study investigated the ameliorating potential of Chenopodium album L. and vitamin C against mercuric chloride-induced oxidative deterioration of reproductive functions in adult male rats. METHODS: Group 1 (control) received saline. Group 2 received Mercury (0.15 mg/kg b.w, i.p) dissolved in distilled water. Groups 3 and 4 were given oral gavage of vitamin C (200 mg/kg b.w) and the ethanolic extract of C. album (200 mg/kg b.w) respectively, along with Mercury (0.15 mg/kg b.w, i.p). Group 5 was treated only with C. album (200 mg/kg b.w). After 30 days of the treatment, the rats were dissected and their testicular tissue and the cauda epididymis were used for biochemical analysis while blood plasma was used for protein determination. RESULTS: The applied dose-treatment of Mercury-induced oxidative stress in the testis and cauda epididymis tissues of the rats was apparent by a noteworthy decrease in total protein, CAT, SOD, POD, and GST values while there was increase in ROS and TBARS levels. Furthermore, Mercury decreases daily sperm production and enhanced sperm DNA damage as noticeable by an increase in the head and tail length of comets and decrease in intact DNA. There was no significant effect on the body weight and the weight of the reproductive tissues. Treatment with C. album significantly ameliorated the total protein, ROS, and TBARS content. Similarly, the level of CAT, SOD, POD, and GST was significantly improved and the daily sperm production was significantly increased. Furthermore, C. album administration significantly protected Mercury-induced sperm DNA damage. The results of the extract treatment group were compared with those of vitamin C in detoxifying the oxidative stress and restoring the sperm parameters. CONCLUSION: C. album showed protection against Mercury-induced oxidative stress by ameliorating antioxidant enzyme activity, daily sperm production, and DNA damage in rat testes. This suggests that C. album could be beneficial against toxicity induced by an environmental toxicant. SN - 1347-4715 UR - https://www.unboundmedicine.com/medline/citation/31759394/Ameliorating_potency_of_Chenopodium_album_Linn._and_vitamin_C_against_mercuric_chloride-induced_oxidative_stress_in_testes_of_Sprague_Dawley_rats L2 - https://environhealthprevmed.biomedcentral.com/articles/10.1186/s12199-019-0820-x DB - PRIME DP - Unbound Medicine ER -