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Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated with Inflammatory/Oxidative Stress Response in Lung Cells.
Chem Res Toxicol. 2020 02 17; 33(2):381-387.CR

Abstract

Exposure to fine particulate matter (PM2.5), of which secondary organic aerosol (SOA) is a major constituent, is linked to adverse health outcomes, including cardiovascular disease, lung cancer, and preterm birth. Atmospheric oxidation of isoprene, the most abundant nonmethane hydrocarbon emitted into Earth's atmosphere primarily from vegetation, contributes to SOA formation. Isoprene-derived SOA has previously been found to alter inflammatory/oxidative stress genes. MicroRNAs (miRNAs) are epigenetic regulators that serve as post-transcriptional modifiers and key mediators of gene expression. To assess whether isoprene-derived SOA alters miRNA expression, BEAS-2B lung cells were exposed to laboratory-generated isoprene-derived SOA constituents derived from the acid-driven multiphase chemistry of authentic methacrylic acid epoxide (MAE) or isomeric isoprene epoxydiols (IEPOX) with acidic sulfate aerosol particles. These IEPOX- and MAE-derived SOA constituents have been shown to be measured in large quantities within PM2.5 collected from isoprene-rich areas affected by acidic sulfate aerosol particles derived from human activities. A total of 29 miRNAs were identified as differentially expressed when exposed to IEPOX-derived SOA and 2 when exposed to MAE-derived SOA, a number of which are inflammatory/oxidative stress associated. These results suggest that miRNAs may modulate the inflammatory/oxidative stress response to SOA exposure, thereby advancing the understanding of airway cell epigenetic response to SOA.

Authors+Show Affiliations

Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.Department of Environmental Sciences , University of California , Riverside , California 92521 , United States.Department of Environmental Studies , Dickinson College , Carlisle , Pennsylvania 17013 , United States.Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.Department of Environmental Sciences & Engineering, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States. Curriculum in Toxicology , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States. Institute for Environmental Health Solutions, Gillings School of Global Public Health , University of North Carolina at Chapel Hill , Chapel Hill , North Carolina 27599 , United States.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

31765140

Citation

Eaves, Lauren A., et al. "Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated With Inflammatory/Oxidative Stress Response in Lung Cells." Chemical Research in Toxicology, vol. 33, no. 2, 2020, pp. 381-387.
Eaves LA, Smeester L, Hartwell HJ, et al. Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated with Inflammatory/Oxidative Stress Response in Lung Cells. Chem Res Toxicol. 2020;33(2):381-387.
Eaves, L. A., Smeester, L., Hartwell, H. J., Lin, Y. H., Arashiro, M., Zhang, Z., Gold, A., Surratt, J. D., & Fry, R. C. (2020). Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated with Inflammatory/Oxidative Stress Response in Lung Cells. Chemical Research in Toxicology, 33(2), 381-387. https://doi.org/10.1021/acs.chemrestox.9b00322
Eaves LA, et al. Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated With Inflammatory/Oxidative Stress Response in Lung Cells. Chem Res Toxicol. 2020 02 17;33(2):381-387. PubMed PMID: 31765140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoprene-Derived Secondary Organic Aerosol Induces the Expression of MicroRNAs Associated with Inflammatory/Oxidative Stress Response in Lung Cells. AU - Eaves,Lauren A, AU - Smeester,Lisa, AU - Hartwell,Hadley J, AU - Lin,Ying-Hsuan, AU - Arashiro,Maiko, AU - Zhang,Zhenfa, AU - Gold,Avram, AU - Surratt,Jason D, AU - Fry,Rebecca C, Y1 - 2019/12/13/ PY - 2019/11/26/pubmed PY - 2021/7/3/medline PY - 2019/11/26/entrez SP - 381 EP - 387 JF - Chemical research in toxicology JO - Chem Res Toxicol VL - 33 IS - 2 N2 - Exposure to fine particulate matter (PM2.5), of which secondary organic aerosol (SOA) is a major constituent, is linked to adverse health outcomes, including cardiovascular disease, lung cancer, and preterm birth. Atmospheric oxidation of isoprene, the most abundant nonmethane hydrocarbon emitted into Earth's atmosphere primarily from vegetation, contributes to SOA formation. Isoprene-derived SOA has previously been found to alter inflammatory/oxidative stress genes. MicroRNAs (miRNAs) are epigenetic regulators that serve as post-transcriptional modifiers and key mediators of gene expression. To assess whether isoprene-derived SOA alters miRNA expression, BEAS-2B lung cells were exposed to laboratory-generated isoprene-derived SOA constituents derived from the acid-driven multiphase chemistry of authentic methacrylic acid epoxide (MAE) or isomeric isoprene epoxydiols (IEPOX) with acidic sulfate aerosol particles. These IEPOX- and MAE-derived SOA constituents have been shown to be measured in large quantities within PM2.5 collected from isoprene-rich areas affected by acidic sulfate aerosol particles derived from human activities. A total of 29 miRNAs were identified as differentially expressed when exposed to IEPOX-derived SOA and 2 when exposed to MAE-derived SOA, a number of which are inflammatory/oxidative stress associated. These results suggest that miRNAs may modulate the inflammatory/oxidative stress response to SOA exposure, thereby advancing the understanding of airway cell epigenetic response to SOA. SN - 1520-5010 UR - https://www.unboundmedicine.com/medline/citation/31765140/Isoprene_Derived_Secondary_Organic_Aerosol_Induces_the_Expression_of_MicroRNAs_Associated_with_Inflammatory/Oxidative_Stress_Response_in_Lung_Cells_ L2 - https://doi.org/10.1021/acs.chemrestox.9b00322 DB - PRIME DP - Unbound Medicine ER -