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Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis.
Plant Biotechnol (Tokyo) 2019; 36(2):107-112PB

Abstract

As major components of the ubiquitin system, ubiquitin ligases mediate the transfer of ubiquitin to specific target substrates, thereby playing important roles in regulating a wide range of cellular processes. The Arabidopsis Tóxicos en Levadura (ATL) family is a group of plant-specific RING-type ubiquitin ligases with N-terminal transmembrane-like domains. To date, 91 ATL isoforms have been identified in the Arabidopsis genome, with some reported to regulate plant responses to environmental stresses. However, the functions of most ATLs remain unclear. This study showed that ATL8 is a sugar starvation response gene and that ATL8 expression was significantly increased by sugar starvation conditions but repressed by exogenous sugar supply. The ATL8 protein was found to possess ubiquitin ligase activity in vitro and to localize to membrane-bound compartments in plant cells. In addition, Starch Synthase 4 was identified as a putative interactor with ATL8, suggesting that ATL8 may be involved in modulating starch accumulation in response to sugar availability. These findings suggest that ATL8 functions as a membrane-localized ubiquitin ligase likely to be involved in the adaptation of Arabidopsis plants to sugar starvation stress.

Authors+Show Affiliations

Faculty of Science and Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.Faculty of Science and Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.Department of Bioinformatics, College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.Faculty of Science and Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.Faculty of Science and Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.Faculty of Science and Graduate School of Life Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31768111

Citation

Luo, Yongming, et al. "Involvement of the Membrane-localized Ubiquitin Ligase ATL8 in Sugar Starvation Response in Arabidopsis." Plant Biotechnology (Tokyo, Japan), vol. 36, no. 2, 2019, pp. 107-112.
Luo Y, Aoyama S, Fukao Y, et al. Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis. Plant Biotechnol (Tokyo). 2019;36(2):107-112.
Luo, Y., Aoyama, S., Fukao, Y., Chiba, Y., Sato, T., & Yamaguchi, J. (2019). Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis. Plant Biotechnology (Tokyo, Japan), 36(2), pp. 107-112. doi:10.5511/plantbiotechnology.19.0328a.
Luo Y, et al. Involvement of the Membrane-localized Ubiquitin Ligase ATL8 in Sugar Starvation Response in Arabidopsis. Plant Biotechnol (Tokyo). 2019;36(2):107-112. PubMed PMID: 31768111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of the membrane-localized ubiquitin ligase ATL8 in sugar starvation response in Arabidopsis. AU - Luo,Yongming, AU - Aoyama,Shoki, AU - Fukao,Yoichiro, AU - Chiba,Yukako, AU - Sato,Takeo, AU - Yamaguchi,Junji, PY - 2019/11/27/entrez PY - 2019/11/27/pubmed PY - 2019/11/27/medline KW - SnRK1 KW - starch synthase KW - sugar signaling KW - ubiquitination SP - 107 EP - 112 JF - Plant biotechnology (Tokyo, Japan) JO - Plant Biotechnol (Tokyo) VL - 36 IS - 2 N2 - As major components of the ubiquitin system, ubiquitin ligases mediate the transfer of ubiquitin to specific target substrates, thereby playing important roles in regulating a wide range of cellular processes. The Arabidopsis Tóxicos en Levadura (ATL) family is a group of plant-specific RING-type ubiquitin ligases with N-terminal transmembrane-like domains. To date, 91 ATL isoforms have been identified in the Arabidopsis genome, with some reported to regulate plant responses to environmental stresses. However, the functions of most ATLs remain unclear. This study showed that ATL8 is a sugar starvation response gene and that ATL8 expression was significantly increased by sugar starvation conditions but repressed by exogenous sugar supply. The ATL8 protein was found to possess ubiquitin ligase activity in vitro and to localize to membrane-bound compartments in plant cells. In addition, Starch Synthase 4 was identified as a putative interactor with ATL8, suggesting that ATL8 may be involved in modulating starch accumulation in response to sugar availability. These findings suggest that ATL8 functions as a membrane-localized ubiquitin ligase likely to be involved in the adaptation of Arabidopsis plants to sugar starvation stress. SN - 1342-4580 UR - https://www.unboundmedicine.com/medline/citation/31768111/Involvement_of_the_membrane-localized_ubiquitin_ligase_ATL8_in_sugar_starvation_response_in_Arabidopsis L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31768111/ DB - PRIME DP - Unbound Medicine ER -