TY - JOUR T1 - Chang'an II Decoction (II)-Containing Serum Ameliorates Tumor Necrosis Factor-α-Induced Intestinal Epithelial Barrier Dysfunction via MLCK-MLC Signaling Pathway in Rats. AU - Chen,Ting, AU - Yin,Xiao-Lan, AU - Kang,Nan, AU - Wang,Xiao-Ge, AU - Li,Bao-Shuang, AU - Ji,Hai-Jie, AU - Zhang,Yin-Qiang, AU - Bian,Li-Qun, AU - Zhang,Bei-Hua, AU - Wang,Feng-Yun, AU - Tang,Xu-Dong, Y1 - 2019/11/25/ PY - 2018/04/19/accepted PY - 2019/11/27/pubmed PY - 2021/7/3/medline PY - 2019/11/27/entrez KW - Chang’an II Decoction KW - drug-containing serum KW - intestinal epithelial cells KW - myosin light chain kinase-myosin light chain KW - signaling pathway KW - tight junction KW - tumor necrosis factor-α SP - 745 EP - 753 JF - Chinese journal of integrative medicine JO - Chin J Integr Med VL - 26 IS - 10 N2 - OBJECTIVE: To investigate the effect of Chang'an II Decoction (II))-containing serum on intestinal epithelial barrier dysfunction in rats. METHODS: Tumor necrosis factor (TNF)-α-induced injury of Caco-2 monolayers were established as an inflammatory model of human intestinal epithelium. Caco-2 monolayers were treated with blank serum and Chang'an II Decoction-containing serum that obtained from the rats which were treated with distilled water and Chang'an II Decoction intragastrically at doses of 0.49, 0.98, 1.96 g/(kg·d) for 1 week, respectively. After preparation of containing serum, cells were divided into the normal group, the model group, the Chang'an II-H, M, and L groups (treated with 30 ng/mL TNF-α and medium plus 10% high, middle-, and low-doses Chang'an II serum, respectively). Epithelial barrier function was assessed by transepithelial electrical resistance (TER) and permeability of fluorescein isothiocyanate (FITC)-labeled dextran. Transmission electron microscopy was used to observe the ultrastructure of tight junctions (TJs). Immunofluorescence of zonula occludens-1 (ZO-1), claudin-1 and nuclear transcription factor-kappa p65 (NF-κ Bp65) were measured to determine the protein distribution. The mRNA expression of myosin light chain kinase (MLCK) was measured by real-time polymerase chain reaction. The expression levels of MLCK, myosin light chain (MLC) and p-MLC were determined by Western blot. RESULTS: Chang'an II Decoction-containing serum significantly attenuated the TER and paracellular permeability induced by TNF-α. It alleviated TNF-α-induced morphological alterations in TJ proteins. The increases in MLCK mRNA and MLCK, MLC and p-MLC protein expressions induced by TNF-α were significantly inhibited in the Chang'an II-H group. Additionally, Chang'an II Decoction significantly attenuated translocation of NF-κ Bp65 into the nucleus. CONCLUSION: High-dose Chang'an II-containing serum attenuates TNF-α-induced intestinal barrier dysfunction. The underlying mechanism may be involved in inhibiting the MLCK-MLC phosphorylation signaling pathway mediated by NF-κ Bp65. SN - 1672-0415 UR - https://www.unboundmedicine.com/medline/citation/31768870/Chang'an_II_Decoction__II__Containing_Serum_Ameliorates_Tumor_Necrosis_Factor_��_Induced_Intestinal_Epithelial_Barrier_Dysfunction_via_MLCK_MLC_Signaling_Pathway_in_Rats_ DB - PRIME DP - Unbound Medicine ER -