Tags

Type your tag names separated by a space and hit enter

An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in Leptodactylus latinasus tadpoles (Anura: Leptodactylidae).
Environ Sci Pollut Res Int. 2020 Jan; 27(2):2131-2143.ES

Abstract

Genotoxic, biochemical, and individual organizational effects on Leptodactylus latinasus tadpoles were evaluated after exposure to an imazethapyr (IMZT)-based commercial herbicide formulation, Pivot® H (10.59% IMZT). A determination of the value of the lethal concentration (LC50) was determined as a toxicological endpoint. Alterations in animal behavior and morphological abnormalities as well as cholinesterase (ChE), catalase (CAT), and glutathione S-transferase (GST) activities were employed as individual sublethal endpoints. Micronuclei frequencies (MNs), binucleated cells (BNs), blebbed nuclei (BLs), lobed nuclei (LBs), notched nuclei (NTs), erythroplastids (EPs), and evaluation of DNA strand breaks were employed as genotoxic endpoints. All biomarkers were evaluated after 48 and 96 h of exposure to concentrations of IMZT within 0.07-4.89 mg/L. LC5096h values of 1.01 and 0.29 mg/L IMZT were obtained for Gosner stages 25 and 36, respectively. Irregular swimming, diamond body shape, and decreased frequency of keratodonts were detected at both sampling times. Results showed that IMZT increased GST activity and MN frequency at 48 and 96 h of exposure. Other nuclear abnormalities were also observed in the circulating erythrocytes of tadpoles, i.e., NT and BL values after 48 h, and LN, BL, and EP values after 96 h. Finally, results showed that IMZT within 0.07-0.22 mg/L increased the genetic damage index in tadpoles exposed for both exposure times (48 and 96 h). This study is the first to report the sublethal biochemical effects of IMZT in anurans and is also the first report using L. latinasus tadpoles as a bioindicator for ecotoxicological studies.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 No. 3 (esq. 120), B1904AMA, La Plata, Argentina. Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Instituto de Recursos Biológicos, Centro de Investigaciones de Recursos Naturales (CIRN), Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 No. 3 (esq. 120), B1904AMA, La Plata, Argentina. marcelo.larramendy@gmail.com. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. marcelo.larramendy@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31773531

Citation

Pérez-Iglesias, Juan M., et al. "An Imazethapyr-based Herbicide Formulation Induces Genotoxic, Biochemical, and Individual Organizational Effects in Leptodactylus Latinasus Tadpoles (Anura: Leptodactylidae)." Environmental Science and Pollution Research International, vol. 27, no. 2, 2020, pp. 2131-2143.
Pérez-Iglesias JM, Brodeur JC, Larramendy ML. An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in Leptodactylus latinasus tadpoles (Anura: Leptodactylidae). Environ Sci Pollut Res Int. 2020;27(2):2131-2143.
Pérez-Iglesias, J. M., Brodeur, J. C., & Larramendy, M. L. (2020). An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in Leptodactylus latinasus tadpoles (Anura: Leptodactylidae). Environmental Science and Pollution Research International, 27(2), 2131-2143. https://doi.org/10.1007/s11356-019-06880-7
Pérez-Iglesias JM, Brodeur JC, Larramendy ML. An Imazethapyr-based Herbicide Formulation Induces Genotoxic, Biochemical, and Individual Organizational Effects in Leptodactylus Latinasus Tadpoles (Anura: Leptodactylidae). Environ Sci Pollut Res Int. 2020;27(2):2131-2143. PubMed PMID: 31773531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in Leptodactylus latinasus tadpoles (Anura: Leptodactylidae). AU - Pérez-Iglesias,Juan M, AU - Brodeur,Julie C, AU - Larramendy,Marcelo L, Y1 - 2019/11/26/ PY - 2019/07/11/received PY - 2019/10/24/accepted PY - 2019/11/28/pubmed PY - 2020/4/10/medline PY - 2019/11/28/entrez KW - Genotoxicity KW - Imazethapyr herbicide formulation KW - Lethality KW - Morphological and behavioral alterations KW - Oxidative stress SP - 2131 EP - 2143 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 27 IS - 2 N2 - Genotoxic, biochemical, and individual organizational effects on Leptodactylus latinasus tadpoles were evaluated after exposure to an imazethapyr (IMZT)-based commercial herbicide formulation, Pivot® H (10.59% IMZT). A determination of the value of the lethal concentration (LC50) was determined as a toxicological endpoint. Alterations in animal behavior and morphological abnormalities as well as cholinesterase (ChE), catalase (CAT), and glutathione S-transferase (GST) activities were employed as individual sublethal endpoints. Micronuclei frequencies (MNs), binucleated cells (BNs), blebbed nuclei (BLs), lobed nuclei (LBs), notched nuclei (NTs), erythroplastids (EPs), and evaluation of DNA strand breaks were employed as genotoxic endpoints. All biomarkers were evaluated after 48 and 96 h of exposure to concentrations of IMZT within 0.07-4.89 mg/L. LC5096h values of 1.01 and 0.29 mg/L IMZT were obtained for Gosner stages 25 and 36, respectively. Irregular swimming, diamond body shape, and decreased frequency of keratodonts were detected at both sampling times. Results showed that IMZT increased GST activity and MN frequency at 48 and 96 h of exposure. Other nuclear abnormalities were also observed in the circulating erythrocytes of tadpoles, i.e., NT and BL values after 48 h, and LN, BL, and EP values after 96 h. Finally, results showed that IMZT within 0.07-0.22 mg/L increased the genetic damage index in tadpoles exposed for both exposure times (48 and 96 h). This study is the first to report the sublethal biochemical effects of IMZT in anurans and is also the first report using L. latinasus tadpoles as a bioindicator for ecotoxicological studies. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/31773531/An_imazethapyr_based_herbicide_formulation_induces_genotoxic_biochemical_and_individual_organizational_effects_in_Leptodactylus_latinasus_tadpoles__Anura:_Leptodactylidae__ L2 - https://dx.doi.org/10.1007/s11356-019-06880-7 DB - PRIME DP - Unbound Medicine ER -