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Serological and molecular detection of infection with Mycobacterium leprae in Brazilian six banded armadillos (Euphractus sexcinctus).
Comp Immunol Microbiol Infect Dis. 2020 Feb; 68:101397.CI

Abstract

Leprosy was recognized as a zoonotic disease, associated with nine-banded armadillos (Dasypus novemcinctus) in the Southern United States of America in 2011. In addition, there is growing evidence to support a role for armadillos in zoonotic leprosy in South America. The current study evaluated twenty specimens of the six-banded armadillo (Euphractus sexcinctus), collected from rural locations in the state of Rio Grande do Norte (RN), Brazil for evidence of infection with Mycobacterium leprae. Serum was examined using two "in-house" enzyme-linked immunosorbent assays (ELISAs) and via two commercially available (ML flow and NDO-LID®) immunochromatographic lateral flow (LF) tests, for detection of the PGL-I and/or LID-1 antigens of the bacterium. The presence of M. leprae DNA in liver tissue was examined using the multi-copy, M. leprae-specific repetitive element (RLEP), as target in conventional and nested PCR assays. Molecular and anti-PGL-I-ELISA data indicated that 20/20 (100 %) of the armadillos were infected with M. leprae. The corresponding detection levels recorded with the LF tests were 17/20 (85 %) and 16/20 (85 %), for the NDO-LID® and ML flow tests, respectively. Our results indicate that, in common with D. novemcinctus, six banded armadillos (a species hunted and reared as a food-source in some regions of Brazil, including RN), represent a potential reservoir of M. leprae and as such, their role in a possible zoonotic cycle of leprosy within Brazil warrants further investigation.

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Publisher Full Text (DOI)

Authors+Show Affiliations

da Silva Ferreira J
Multiuser Molecular Biology Laboratory, Department of Animal Parasitology, Institute of Veterinary Medicine, Federal Rural University of Rio de Janeiro, Seropédica, Brazil.
de Carvalho FM
Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Vidal Pessolani MC
Laboratory of Cellular Microbiology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
de Paula Antunes JMA
Veterinary Hospital, Federal Rural Universityof Semi-Árido, Rio Grande do Norte, Brazil.
de Medeiros Oliveira IVP
Veterinary Hospital, Federal Rural Universityof Semi-Árido, Rio Grande do Norte, Brazil.
Ferreira Moura GH
Veterinary Hospital, Federal Rural Universityof Semi-Árido, Rio Grande do Norte, Brazil.
Truman RW
Department of Health and Humans Services, Health Resources and Services Administration, Healthcare Systems Bureau, National Hansen's Disease Program, Baton Rouge, United States.
Peña MT
Department of Health and Humans Services, Health Resources and Services Administration, Healthcare Systems Bureau, National Hansen's Disease Program, Baton Rouge, United States.
Sharma R
Department of Health and Humans Services, Health Resources and Services Administration, Healthcare Systems Bureau, National Hansen's Disease Program, Baton Rouge, United States.
Duthie MS
Infectious Disease Research Institute, Seattle, United States.
de Paula Souza E Guimarães RJ
Evandro Chagas Institute, Health Surveillance Secretariat, Brazilian Ministry of Health, Ananindeua, Pará, Brazil.
Nogueira Brum Fontes A
Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
NoelSuffys P
Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
McIntosh D
Multiuser Molecular Biology Laboratory, Department of Animal Parasitology, Institute of Veterinary Medicine, Federal Rural University of Rio de Janeiro, Seropédica, Brazil. Electronic address: pus972@yahoo.co.uk.

MeSH

AnimalsArmadillosBrazilDisease ReservoirsEnzyme-Linked Immunosorbent AssayFemaleLeprosyMaleMycobacterium lepraePolymerase Chain ReactionZoonoses

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31775113

Citation

da Silva Ferreira, Jéssica, et al. "Serological and Molecular Detection of Infection With Mycobacterium Leprae in Brazilian Six Banded Armadillos (Euphractus Sexcinctus)." Comparative Immunology, Microbiology and Infectious Diseases, vol. 68, 2020, p. 101397.
da Silva Ferreira J, de Carvalho FM, Vidal Pessolani MC, et al. Serological and molecular detection of infection with Mycobacterium leprae in Brazilian six banded armadillos (Euphractus sexcinctus). Comp Immunol Microbiol Infect Dis. 2020;68:101397.
da Silva Ferreira, J., de Carvalho, F. M., Vidal Pessolani, M. C., de Paula Antunes, J. M. A., de Medeiros Oliveira, I. V. P., Ferreira Moura, G. H., Truman, R. W., Peña, M. T., Sharma, R., Duthie, M. S., de Paula Souza E Guimarães, R. J., Nogueira Brum Fontes, A., NoelSuffys, P., & McIntosh, D. (2020). Serological and molecular detection of infection with Mycobacterium leprae in Brazilian six banded armadillos (Euphractus sexcinctus). Comparative Immunology, Microbiology and Infectious Diseases, 68, 101397. https://doi.org/10.1016/j.cimid.2019.101397
da Silva Ferreira J, et al. Serological and Molecular Detection of Infection With Mycobacterium Leprae in Brazilian Six Banded Armadillos (Euphractus Sexcinctus). Comp Immunol Microbiol Infect Dis. 2020;68:101397. PubMed PMID: 31775113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serological and molecular detection of infection with Mycobacterium leprae in Brazilian six banded armadillos (Euphractus sexcinctus). AU - da Silva Ferreira,Jéssica, AU - de Carvalho,Fernanda Marques, AU - Vidal Pessolani,Maria Cristina, AU - de Paula Antunes,João Marcelo Azevedo, AU - de Medeiros Oliveira,Ilanna Vanessa Pristo, AU - Ferreira Moura,Gabriela Hémylin, AU - Truman,Richard Wayne, AU - Peña,Maria Teresa, AU - Sharma,Rahul, AU - Duthie,Malcolm S, AU - de Paula Souza E Guimarães,Ricardo José, AU - Nogueira Brum Fontes,Amanda, AU - NoelSuffys,Philip, AU - McIntosh,Douglas, Y1 - 2019/11/18/ PY - 2019/09/16/received PY - 2019/11/10/revised PY - 2019/11/14/accepted PY - 2019/11/28/pubmed PY - 2020/10/10/medline PY - 2019/11/28/entrez KW - Armadillo KW - Immunodiagnostics KW - Leprosy KW - PCR KW - Zoonotic SP - 101397 EP - 101397 JF - Comparative immunology, microbiology and infectious diseases JO - Comp Immunol Microbiol Infect Dis VL - 68 N2 - Leprosy was recognized as a zoonotic disease, associated with nine-banded armadillos (Dasypus novemcinctus) in the Southern United States of America in 2011. In addition, there is growing evidence to support a role for armadillos in zoonotic leprosy in South America. The current study evaluated twenty specimens of the six-banded armadillo (Euphractus sexcinctus), collected from rural locations in the state of Rio Grande do Norte (RN), Brazil for evidence of infection with Mycobacterium leprae. Serum was examined using two "in-house" enzyme-linked immunosorbent assays (ELISAs) and via two commercially available (ML flow and NDO-LID®) immunochromatographic lateral flow (LF) tests, for detection of the PGL-I and/or LID-1 antigens of the bacterium. The presence of M. leprae DNA in liver tissue was examined using the multi-copy, M. leprae-specific repetitive element (RLEP), as target in conventional and nested PCR assays. Molecular and anti-PGL-I-ELISA data indicated that 20/20 (100 %) of the armadillos were infected with M. leprae. The corresponding detection levels recorded with the LF tests were 17/20 (85 %) and 16/20 (85 %), for the NDO-LID® and ML flow tests, respectively. Our results indicate that, in common with D. novemcinctus, six banded armadillos (a species hunted and reared as a food-source in some regions of Brazil, including RN), represent a potential reservoir of M. leprae and as such, their role in a possible zoonotic cycle of leprosy within Brazil warrants further investigation. SN - 1878-1667 UR - https://www.unboundmedicine.com/medline/citation/31775113/Serological_and_molecular_detection_of_infection_with_Mycobacterium_leprae_in_Brazilian_six_banded_armadillos__Euphractus_sexcinctus__ DB - PRIME DP - Unbound Medicine ER -