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Tong-Xie-Yao-Fang improves intestinal permeability in diarrhoea-predominant irritable bowel syndrome rats by inhibiting the NF-κB and notch signalling pathways.
BMC Complement Altern Med. 2019 Nov 27; 19(1):337.BC

Abstract

BACKGROUND

Tong-Xie-Yao-Fang (TXYF) has been shown to be effective in diarrhoea-predominant irritable bowel syndrome (IBS-D) patients. However, the underlying mechanism remains to be clarified. The aim of this study was to investigate the efficacy and related mechanisms of TXYF in an IBS-D rat model.

METHODS

The IBS-D rat model was established with 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. Then, IBS-D rats were divided into control, TXYF and rifaximin groups and treated intragastrically with normal saline, TXYF and rifaximin, respectively, for 14 days. The following indicators were measured before and after treatment: defecation frequency, faecal water content (FWC) and colorectal distension (CRD). Histopathological changes in the distal colon were observed after treatment. The expression of OCLN and ZO1 in the distal colon of IBS-D rats reflected the intestinal mucosal permeability, as measured by qRT-PCR, western blot, and enzyme-linked immunosorbent assays (ELISAs). The NF-κB and Notch signalling pathways and inflammation-related factors were investigated.

RESULTS

After treatment with TXYF, the defecation frequency, FWC and CRD were significantly lower than those in the model group (P < 0.05). HE staining showed that colonic epithelial cells (CECs) in the IBS-D rats displayed significant oedema, impaired intestinal mucosal integrity and an increased influx of inflammatory cells. A significant reduction in granulocyte and CEC oedema was observed after the administration of TXYF and rifaximin compared to that of the model group and blank group (P < 0.05). TXYF significantly upregulated the expression of OCLN and ZO-1 and downregulated inflammation-related factors (IL-6, IL-1β, and TNF-α and the chemokine KC) in IBS-D rats compared to those in the model group rats (P < 0.05). In terms of the NF-κB and Notch signalling pathways, the expression of NICD, p-ERK, Hes-1 and p-P65 decreased significantly in the TXYF and rifaximin groups, while the expression of ATOH1 increased significantly compared to that in the model group (P < 0.05).

CONCLUSION

TXYF can effectively improve intestinal permeability and enhance intestinal mucosal barrier function, which may be related to inhibition of the inflammatory cascade and the NF-κB and Notch signalling pathways.

Links

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Authors+Show Affiliations

Hou Q
Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China. School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China.
Huang Y
Department of Orthopaedics, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Zhu Z
Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China.
Liao L
Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China.
Chen X
Department of Preventive Medicine and Health Statistics, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Han Q
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China. simonhan74@126.com.
Liu F
Department of Gastroenterology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, China. liufb163@126.com.

MeSH

AnimalsCytokinesDiarrheaDisease Models, AnimalDrugs, Chinese HerbalFemaleIntestinal AbsorptionIntestinal MucosaIrritable Bowel SyndromeMaleNF-kappa BRatsReceptors, NotchSignal Transduction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31775739

Citation

Hou, Qiuke, et al. "Tong-Xie-Yao-Fang Improves Intestinal Permeability in Diarrhoea-predominant Irritable Bowel Syndrome Rats By Inhibiting the NF-κB and Notch Signalling Pathways." BMC Complementary and Alternative Medicine, vol. 19, no. 1, 2019, p. 337.
Hou Q, Huang Y, Zhu Z, et al. Tong-Xie-Yao-Fang improves intestinal permeability in diarrhoea-predominant irritable bowel syndrome rats by inhibiting the NF-κB and notch signalling pathways. BMC Complement Altern Med. 2019;19(1):337.
Hou, Q., Huang, Y., Zhu, Z., Liao, L., Chen, X., Han, Q., & Liu, F. (2019). Tong-Xie-Yao-Fang improves intestinal permeability in diarrhoea-predominant irritable bowel syndrome rats by inhibiting the NF-κB and notch signalling pathways. BMC Complementary and Alternative Medicine, 19(1), 337. https://doi.org/10.1186/s12906-019-2749-4
Hou Q, et al. Tong-Xie-Yao-Fang Improves Intestinal Permeability in Diarrhoea-predominant Irritable Bowel Syndrome Rats By Inhibiting the NF-κB and Notch Signalling Pathways. BMC Complement Altern Med. 2019 Nov 27;19(1):337. PubMed PMID: 31775739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tong-Xie-Yao-Fang improves intestinal permeability in diarrhoea-predominant irritable bowel syndrome rats by inhibiting the NF-κB and notch signalling pathways. AU - Hou,Qiuke, AU - Huang,Yongquan, AU - Zhu,Zhaoyang, AU - Liao,Liu, AU - Chen,Xinlin, AU - Han,Quanbin, AU - Liu,Fengbin, Y1 - 2019/11/27/ PY - 2019/08/28/received PY - 2019/11/08/accepted PY - 2019/11/29/entrez PY - 2019/11/30/pubmed PY - 2020/1/22/medline KW - Diarrhoea predominant-irritable bowel syndrome KW - Intestinal permeability KW - NF-κB Signalling KW - Notch Signalling KW - Tong-Xie-Yao-fang SP - 337 EP - 337 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 19 IS - 1 N2 - BACKGROUND: Tong-Xie-Yao-Fang (TXYF) has been shown to be effective in diarrhoea-predominant irritable bowel syndrome (IBS-D) patients. However, the underlying mechanism remains to be clarified. The aim of this study was to investigate the efficacy and related mechanisms of TXYF in an IBS-D rat model. METHODS: The IBS-D rat model was established with 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. Then, IBS-D rats were divided into control, TXYF and rifaximin groups and treated intragastrically with normal saline, TXYF and rifaximin, respectively, for 14 days. The following indicators were measured before and after treatment: defecation frequency, faecal water content (FWC) and colorectal distension (CRD). Histopathological changes in the distal colon were observed after treatment. The expression of OCLN and ZO1 in the distal colon of IBS-D rats reflected the intestinal mucosal permeability, as measured by qRT-PCR, western blot, and enzyme-linked immunosorbent assays (ELISAs). The NF-κB and Notch signalling pathways and inflammation-related factors were investigated. RESULTS: After treatment with TXYF, the defecation frequency, FWC and CRD were significantly lower than those in the model group (P < 0.05). HE staining showed that colonic epithelial cells (CECs) in the IBS-D rats displayed significant oedema, impaired intestinal mucosal integrity and an increased influx of inflammatory cells. A significant reduction in granulocyte and CEC oedema was observed after the administration of TXYF and rifaximin compared to that of the model group and blank group (P < 0.05). TXYF significantly upregulated the expression of OCLN and ZO-1 and downregulated inflammation-related factors (IL-6, IL-1β, and TNF-α and the chemokine KC) in IBS-D rats compared to those in the model group rats (P < 0.05). In terms of the NF-κB and Notch signalling pathways, the expression of NICD, p-ERK, Hes-1 and p-P65 decreased significantly in the TXYF and rifaximin groups, while the expression of ATOH1 increased significantly compared to that in the model group (P < 0.05). CONCLUSION: TXYF can effectively improve intestinal permeability and enhance intestinal mucosal barrier function, which may be related to inhibition of the inflammatory cascade and the NF-κB and Notch signalling pathways. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/31775739/Tong_Xie_Yao_Fang_improves_intestinal_permeability_in_diarrhoea_predominant_irritable_bowel_syndrome_rats_by_inhibiting_the_NF_κB_and_notch_signalling_pathways_ DB - PRIME DP - Unbound Medicine ER -