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[Electroacupuncture improved locomotor function by regulating expression of tyrosine hydroxylase and α-synuclein proteins and transcription activating factor 6 and transcription factor X box binding protein 1 mRNAs in substantia nigra of rats with Parkinson's disease].
Zhen Ci Yan Jiu. 2019 Nov 25; 44(11):805-9.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on behavioral changes, and expression of tyrosine hydroxylase (TH), α-synuclein(α-syn), transcription activating factor 6 (ATF6) and transcription factor X box binding protein 1 (XBP-1) in the substantia nigra of Parkinson's disease (PD) rats, so as to explore its mechanisms underlying improvement of motor function.

METHODS

Thirty-six male SD rats were randomly divided into control, model and EA groups (n=12 rats in each group). The PD model was established by subcutaneous injection of rotenone (2 mg/kg) at the neck and back, once a day for 28 days. EA (2 Hz, 1 mA) was applied to "Fengfu" (GV16) and bilateral "Taichong" (LR3) for 20 min, once a day for 14 successive days. The voluntary motor behavioral changes (total distance, average speed, total movement time, total rest time in 8 min) were detected by open field tests. The immuno-activity of TH and α-syn in the substantia nigra was detected by immunohistochemistry, and the expression of ATF6 mRNA and XBP-1 mRNA detected by fluorescence real-time quantitative PCR.

RESULTS

Following modeling and compared with the control group, the total distance, average speed and total movement time of voluntary movement were significantly decreased (P<0.01), and the total rest time was significantly increased (P<0.01). Moreover, the expression of TH was significantly decreased (P<0.01), and that of α-syn protein, ATF6 mRNA and XBP-1 mRNA significantly increased in the model group in comparison with the control group (P<0.01). After the intervention, the total distance, average speed, and total movement time of voluntary movement in the EA group were considerably higher than those in the model group (P<0.01), and the total rest time was obviously decreased in the EA group (P<0.01). The expression level of TH was significantly increased (P<0.01), and those of α-syn, ATF6 mRNA and XBP-1 mRNA were notably decreased in the EA group compared with the model group (P<0.01).

CONCLUSION

EA intervention can improve the locomotor function in PD model rats, which is associated with its functions in up-regulating the expression of TH protein and down-regulating the expression of α-syn protein, and ATF6 mRNA and XBP-1 mRNA in the substantia nigra of mesencephalon.

Authors+Show Affiliations

College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.College of Acupuncture-moxibustion and Orthopedics, Hubei University of Chinese Medicine/Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, Wuhan 430065, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

31777229

Citation

Ma, Jun, et al. "[Electroacupuncture Improved Locomotor Function By Regulating Expression of Tyrosine Hydroxylase and Α-synuclein Proteins and Transcription Activating Factor 6 and Transcription Factor X Box Binding Protein 1 mRNAs in Substantia Nigra of Rats With Parkinson's Disease]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 44, no. 11, 2019, pp. 805-9.
Ma J, Yuan L, Wang SJ, et al. [Electroacupuncture improved locomotor function by regulating expression of tyrosine hydroxylase and α-synuclein proteins and transcription activating factor 6 and transcription factor X box binding protein 1 mRNAs in substantia nigra of rats with Parkinson's disease]. Zhen Ci Yan Jiu. 2019;44(11):805-9.
Ma, J., Yuan, L., Wang, S. J., Lei, J., Wang, Y., Li, Y. N., & Yu, B. L. (2019). [Electroacupuncture improved locomotor function by regulating expression of tyrosine hydroxylase and α-synuclein proteins and transcription activating factor 6 and transcription factor X box binding protein 1 mRNAs in substantia nigra of rats with Parkinson's disease]. Zhen Ci Yan Jiu = Acupuncture Research, 44(11), 805-9. https://doi.org/10.13702/j.1000-0607.180600
Ma J, et al. [Electroacupuncture Improved Locomotor Function By Regulating Expression of Tyrosine Hydroxylase and Α-synuclein Proteins and Transcription Activating Factor 6 and Transcription Factor X Box Binding Protein 1 mRNAs in Substantia Nigra of Rats With Parkinson's Disease]. Zhen Ci Yan Jiu. 2019 Nov 25;44(11):805-9. PubMed PMID: 31777229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture improved locomotor function by regulating expression of tyrosine hydroxylase and α-synuclein proteins and transcription activating factor 6 and transcription factor X box binding protein 1 mRNAs in substantia nigra of rats with Parkinson's disease]. AU - Ma,Jun, AU - Yuan,Li, AU - Wang,Shu-Ju, AU - Lei,Jun, AU - Wang,Yao, AU - Li,Ya-Nan, AU - Yu,Ben-Lu, PY - 2019/11/29/entrez PY - 2019/11/30/pubmed PY - 2019/12/4/medline KW - Electroacupuncture KW - Endoplasmic reticulum stress KW - Parkinson's disease KW - Transcription activating factor 6 KW - Transcription factor X box binding protein 1 SP - 805 EP - 9 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 44 IS - 11 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on behavioral changes, and expression of tyrosine hydroxylase (TH), α-synuclein(α-syn), transcription activating factor 6 (ATF6) and transcription factor X box binding protein 1 (XBP-1) in the substantia nigra of Parkinson's disease (PD) rats, so as to explore its mechanisms underlying improvement of motor function. METHODS: Thirty-six male SD rats were randomly divided into control, model and EA groups (n=12 rats in each group). The PD model was established by subcutaneous injection of rotenone (2 mg/kg) at the neck and back, once a day for 28 days. EA (2 Hz, 1 mA) was applied to "Fengfu" (GV16) and bilateral "Taichong" (LR3) for 20 min, once a day for 14 successive days. The voluntary motor behavioral changes (total distance, average speed, total movement time, total rest time in 8 min) were detected by open field tests. The immuno-activity of TH and α-syn in the substantia nigra was detected by immunohistochemistry, and the expression of ATF6 mRNA and XBP-1 mRNA detected by fluorescence real-time quantitative PCR. RESULTS: Following modeling and compared with the control group, the total distance, average speed and total movement time of voluntary movement were significantly decreased (P<0.01), and the total rest time was significantly increased (P<0.01). Moreover, the expression of TH was significantly decreased (P<0.01), and that of α-syn protein, ATF6 mRNA and XBP-1 mRNA significantly increased in the model group in comparison with the control group (P<0.01). After the intervention, the total distance, average speed, and total movement time of voluntary movement in the EA group were considerably higher than those in the model group (P<0.01), and the total rest time was obviously decreased in the EA group (P<0.01). The expression level of TH was significantly increased (P<0.01), and those of α-syn, ATF6 mRNA and XBP-1 mRNA were notably decreased in the EA group compared with the model group (P<0.01). CONCLUSION: EA intervention can improve the locomotor function in PD model rats, which is associated with its functions in up-regulating the expression of TH protein and down-regulating the expression of α-syn protein, and ATF6 mRNA and XBP-1 mRNA in the substantia nigra of mesencephalon. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/31777229/[Electroacupuncture_improved_locomotor_function_by_regulating_expression_of_tyrosine_hydroxylase_and_α_synuclein_proteins_and_transcription_activating_factor_6_and_transcription_factor_X_box_binding_protein_1_mRNAs_in_substantia_nigra_of_rats_with_Parkinson's_disease]_ L2 - https://medlineplus.gov/parkinsonsdisease.html DB - PRIME DP - Unbound Medicine ER -