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Maximal exercise and erythrocyte epoxy fatty acids: a lipidomics study.
Physiol Rep. 2019 11; 7(22):e14275.PR

Abstract

Fatty acid (FA)-derived lipid products generated by cytochrome P450 (CYP), lipoxygenase (LOX), and cyclo-oxygenase (COX) influence cardiovascular function. However, plasma measurements invariably ignore 40% of the blood specimen, namely the erythrocytes. These red blood cells (RBCs) represent a cell mass of about 3 kg. RBCs are a potential reservoir for epoxy fatty acids, which on release could regulate vascular capacity. We tested the hypothesis that maximal physical activity would influence the epoxy fatty acid status in RBCs. We used a standardized maximal treadmill exercise according to Bruce to ensure a robust hemodynamic and metabolic response. Central hemodynamic monitoring was performed using blood pressure and heart rate measurements and maximal workload was assessed in metabolic equivalents (METs). We used tandem mass spectrometry (LC-MS/MS) to measure epoxides derived from CYP monooxygenase, as well as metabolites derived from LOX, COX, and CYP hydroxylase pathways. Venous blood was obtained for RBC lipidomics. With the incremental exercise test, increases in the levels of various CYP epoxy-mediators in RBCs, including epoxyoctadecenoic acids (9,10-EpOME, 12,13-EpOME), epoxyeicosatrienoic acids (5,6-EET, 11,12-EET, 14,15-EET), and epoxydocosapentaenoic acids (16,17-EDP, 19,20-EDP) occurred, as heart rate, systolic blood pressure, and plasma lactate concentrations increased. Maximal (13.5 METs) exercise intensity had no effect on diols and various LOX, COX, and hydroxylase mediators. Our findings suggest that CYP epoxy-metabolites could contribute to the cardiovascular response to maximal exercise.

Authors+Show Affiliations

Experimental and Clinical Research Center (ECRC), a joint institution between the Charité University Medicine and Max Delbrück Center (MDC) for Molecular Medicine, Berlin-Buch, Germany. HELIOS Klinikum Berlin-Buch, Berlin, Germany.Experimental and Clinical Research Center (ECRC), a joint institution between the Charité University Medicine and Max Delbrück Center (MDC) for Molecular Medicine, Berlin-Buch, Germany. Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Berlin, Germany.LIPIDOMIX GmbH, Berlin, Germany.LIPIDOMIX GmbH, Berlin, Germany.Experimental and Clinical Research Center (ECRC), a joint institution between the Charité University Medicine and Max Delbrück Center (MDC) for Molecular Medicine, Berlin-Buch, Germany. Nephrology/Intensive Care Section, Charité Campus Virchow, Berlin, Germany.Nephrology/Intensive Care Section, Charité Campus Virchow, Berlin, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31782268

Citation

Gollasch, Benjamin, et al. "Maximal Exercise and Erythrocyte Epoxy Fatty Acids: a Lipidomics Study." Physiological Reports, vol. 7, no. 22, 2019, pp. e14275.
Gollasch B, Wu G, Dogan I, et al. Maximal exercise and erythrocyte epoxy fatty acids: a lipidomics study. Physiol Rep. 2019;7(22):e14275.
Gollasch, B., Wu, G., Dogan, I., Rothe, M., Gollasch, M., & Luft, F. C. (2019). Maximal exercise and erythrocyte epoxy fatty acids: a lipidomics study. Physiological Reports, 7(22), e14275. https://doi.org/10.14814/phy2.14275
Gollasch B, et al. Maximal Exercise and Erythrocyte Epoxy Fatty Acids: a Lipidomics Study. Physiol Rep. 2019;7(22):e14275. PubMed PMID: 31782268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maximal exercise and erythrocyte epoxy fatty acids: a lipidomics study. AU - Gollasch,Benjamin, AU - Wu,Guanlin, AU - Dogan,Inci, AU - Rothe,Michael, AU - Gollasch,Maik, AU - Luft,Friedrich C, PY - 2019/07/30/received PY - 2019/09/10/revised PY - 2019/09/10/accepted PY - 2019/11/30/entrez PY - 2019/11/30/pubmed PY - 2020/8/18/medline KW - Exercise KW - eicosanoids KW - lipidomics KW - red blood cells SP - e14275 EP - e14275 JF - Physiological reports JO - Physiol Rep VL - 7 IS - 22 N2 - Fatty acid (FA)-derived lipid products generated by cytochrome P450 (CYP), lipoxygenase (LOX), and cyclo-oxygenase (COX) influence cardiovascular function. However, plasma measurements invariably ignore 40% of the blood specimen, namely the erythrocytes. These red blood cells (RBCs) represent a cell mass of about 3 kg. RBCs are a potential reservoir for epoxy fatty acids, which on release could regulate vascular capacity. We tested the hypothesis that maximal physical activity would influence the epoxy fatty acid status in RBCs. We used a standardized maximal treadmill exercise according to Bruce to ensure a robust hemodynamic and metabolic response. Central hemodynamic monitoring was performed using blood pressure and heart rate measurements and maximal workload was assessed in metabolic equivalents (METs). We used tandem mass spectrometry (LC-MS/MS) to measure epoxides derived from CYP monooxygenase, as well as metabolites derived from LOX, COX, and CYP hydroxylase pathways. Venous blood was obtained for RBC lipidomics. With the incremental exercise test, increases in the levels of various CYP epoxy-mediators in RBCs, including epoxyoctadecenoic acids (9,10-EpOME, 12,13-EpOME), epoxyeicosatrienoic acids (5,6-EET, 11,12-EET, 14,15-EET), and epoxydocosapentaenoic acids (16,17-EDP, 19,20-EDP) occurred, as heart rate, systolic blood pressure, and plasma lactate concentrations increased. Maximal (13.5 METs) exercise intensity had no effect on diols and various LOX, COX, and hydroxylase mediators. Our findings suggest that CYP epoxy-metabolites could contribute to the cardiovascular response to maximal exercise. SN - 2051-817X UR - https://www.unboundmedicine.com/medline/citation/31782268/Maximal_exercise_and_erythrocyte_epoxy_fatty_acids:_a_lipidomics_study_ L2 - https://doi.org/10.14814/phy2.14275 DB - PRIME DP - Unbound Medicine ER -