Tags

Type your tag names separated by a space and hit enter

Distinct redox signalling and nickel tolerance in Brassica juncea and Arabidopsis thaliana.
Ecotoxicol Environ Saf. 2020 Feb; 189:109989.EE

Abstract

Despite of its essentiality, nickel (Ni) in excess is toxic for plants partly due to the overproduction of reactive oxygen species (ROS) and the consequent increase in oxidative stress signalling. However, in Ni-stressed plants little is known about the signal transduction of reactive nitrogen species (RNS) and protein tyrosine nitration as the protein-level consequence of increased RNS formation. Our experiments compared the nickel accumulation and tolerance, the redox signalling and the protein nitration in the agar-grown Arabidopsis thaliana and Brassica juncea exposed to Ni (50 μM nickel chloride). Studying GUS-tagged Arabidopsis lines (ARR5::GUS, ACS8::GUS and DR5::GUS) revealed that Ni-increased lateral root (LR) emergence, and concomitantly reduced LR initiation were accompanied by elevated levels of auxin, cytokinin, and ethylene in the LRs or in upper root parts, whereas Ni-induced primary root shortening is related to decreased auxin, and increased cytokinin and ethylene levels. These suggest the Ni-induced disturbance of hormonal balance in the root system. Results of the comparative study showed that weaker Ni tolerance of A. thaliana was coupled with a Ni-induced increase in RNS, ROS, and hydrogen sulfide levels, as well as with an increase in redox signalling and consequent increment of protein nitration. However, in relative Ni tolerant B. juncea, redox signalling (except for peroxynitrite) was not modified, and Ni-induced intensification of protein tyrosine nitration was less pronounced. Data collectively show that the better Ni tolerance of Brassica juncea may be related to the capability of preventing the induction of redox signalling and consequently to the slighter increase in protein nitration.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Kolbert Z
Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary. Electronic address: kolzsu@bio.u-szeged.hu.
Oláh D
Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary; Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary. Electronic address: olah.dora18@citromail.hu.
Molnár Á
Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary. Electronic address: molnara@bio.u-szeged.hu.
Szőllősi R
Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary. Electronic address: szoszo@bio.u-szeged.hu.
Erdei L
Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary. Electronic address: erdei@bio.u-szeged.hu.
Ördög A
Department of Plant Biology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary. Electronic address: aordog@bio.u-szeged.hu.

MeSH

ArabidopsisCytokininsEthylenesIndoleacetic AcidsMustard PlantNickelOxidation-ReductionOxidative StressPlant RootsReactive Nitrogen SpeciesReactive Oxygen SpeciesSignal Transduction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31784105

Citation

Kolbert, Zsuzsanna, et al. "Distinct Redox Signalling and Nickel Tolerance in Brassica Juncea and Arabidopsis Thaliana." Ecotoxicology and Environmental Safety, vol. 189, 2020, p. 109989.
Kolbert Z, Oláh D, Molnár Á, et al. Distinct redox signalling and nickel tolerance in Brassica juncea and Arabidopsis thaliana. Ecotoxicol Environ Saf. 2020;189:109989.
Kolbert, Z., Oláh, D., Molnár, Á., Szőllősi, R., Erdei, L., & Ördög, A. (2020). Distinct redox signalling and nickel tolerance in Brassica juncea and Arabidopsis thaliana. Ecotoxicology and Environmental Safety, 189, 109989. https://doi.org/10.1016/j.ecoenv.2019.109989
Kolbert Z, et al. Distinct Redox Signalling and Nickel Tolerance in Brassica Juncea and Arabidopsis Thaliana. Ecotoxicol Environ Saf. 2020;189:109989. PubMed PMID: 31784105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct redox signalling and nickel tolerance in Brassica juncea and Arabidopsis thaliana. AU - Kolbert,Zsuzsanna, AU - Oláh,Dóra, AU - Molnár,Árpád, AU - Szőllősi,Réka, AU - Erdei,László, AU - Ördög,Attila, Y1 - 2019/11/26/ PY - 2019/08/26/received PY - 2019/11/14/revised PY - 2019/11/18/accepted PY - 2019/12/1/pubmed PY - 2020/4/28/medline PY - 2019/12/1/entrez KW - Arabidopsis thaliana KW - Brassica juncea KW - Hormonal imbalance KW - Nickel stress KW - Protein tyrosine nitration KW - Redox signalling SP - 109989 EP - 109989 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 189 N2 - Despite of its essentiality, nickel (Ni) in excess is toxic for plants partly due to the overproduction of reactive oxygen species (ROS) and the consequent increase in oxidative stress signalling. However, in Ni-stressed plants little is known about the signal transduction of reactive nitrogen species (RNS) and protein tyrosine nitration as the protein-level consequence of increased RNS formation. Our experiments compared the nickel accumulation and tolerance, the redox signalling and the protein nitration in the agar-grown Arabidopsis thaliana and Brassica juncea exposed to Ni (50 μM nickel chloride). Studying GUS-tagged Arabidopsis lines (ARR5::GUS, ACS8::GUS and DR5::GUS) revealed that Ni-increased lateral root (LR) emergence, and concomitantly reduced LR initiation were accompanied by elevated levels of auxin, cytokinin, and ethylene in the LRs or in upper root parts, whereas Ni-induced primary root shortening is related to decreased auxin, and increased cytokinin and ethylene levels. These suggest the Ni-induced disturbance of hormonal balance in the root system. Results of the comparative study showed that weaker Ni tolerance of A. thaliana was coupled with a Ni-induced increase in RNS, ROS, and hydrogen sulfide levels, as well as with an increase in redox signalling and consequent increment of protein nitration. However, in relative Ni tolerant B. juncea, redox signalling (except for peroxynitrite) was not modified, and Ni-induced intensification of protein tyrosine nitration was less pronounced. Data collectively show that the better Ni tolerance of Brassica juncea may be related to the capability of preventing the induction of redox signalling and consequently to the slighter increase in protein nitration. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/31784105/Distinct_redox_signalling_and_nickel_tolerance_in_Brassica_juncea_and_Arabidopsis_thaliana_ DB - PRIME DP - Unbound Medicine ER -