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Evaluation of bradykinin, VEGF, and EGF biomarkers in gingival crevicular fluid and comparison of PhotoBioModulation with conventional techniques in periodontitis: a split-mouth randomized clinical trial.

Abstract

Periodontal disease is a chronic progressive inflammatory process leading to damage of tooth-supporting tissues. This comparative study assessed the effect of PhotoBioModulation (PBM) versus conventional therapy, and investigated biomarkers involved in the healing process. The test group comprised twenty systemically-healthy non-smoking subjects with chronic periodontitis with the presence of two matched contro-lateral premolar sites (probing depth > 5 mm); twenty subjects without chronic periodontitis (CP) served as control group. Patients were treated at baseline, either with scaling and root planing (SRP group) or with a procedure entailing SRP supported by PBM (PBM group). The laser used was a diode laser operating at 645 nm wavelength, 10 J/cm2, and 0.5 W/cm2 with a 600 μm fiber optic. Crevicular fluid levels of bradykinin (BK), vascular endothelial growth factor (VEGF), and epidermal growth factor z (EGF) were determined at both sites. Crevicular fluid specimens from both groups were analyzed with the ELISA TEST. Clinical differences in analyzed outcomes were observed in favor of PBM treatment. Taking average values as 100%, the reduction in BK concentration was 47.68% with SRP and 68.43% with PBM on day 3; the VEGF concentration decreased by 35.73% with SRP and 48.59% with PBM on day 7; the EGF concentration increased by 55.58% with SRP and by 58.11% with PBM on day 21.Clinical parameters improved significantly in both groups (pooled mean values of probing depth decreased from 5.6 to 4.5 mm; gingival index from 1.92 to 1.1; and bleeding on probing from 49.67 to 23.23) but did not vary significantly between the PBM and the SRP group. The results confirmed that PBM have beneficial effects in the early phases of the healing process playing a role in modulation of BK, EGF, and VEGF in gingival crevicular fluid levels; both groups had significant clinical improvement over control but there was no significant difference between them, only a trend for PBM group. The overall results of the study suggest a potential benefit of PBM in conjunction with SRP in treating chronic periodontitis.

Authors+Show Affiliations

Department of Medical Sciences and Diagnostic Integrated, University of Genoa, Genova, Italy.Department of Medical Sciences and Diagnostic Integrated, University of Genoa, Genova, Italy. Alessandro.ugolini@unige.it.Dental School, Vita-Salute San Raffaele University, Milan, Italy.Dental School, Vita-Salute San Raffaele University, Milan, Italy.Department of Medical Sciences and Diagnostic Integrated, University of Genoa, Genova, Italy.Section of Biostatistics, Department of Health Sciences (DISSAL), University of Genoa, Genoa, Italy.Department of Biomedical, Surgical and Dental Sciences, University of Milan, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milan, Italy.Dental School, Vita-Salute San Raffaele University, Milan, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31784929

Citation

Angiero, Francesca, et al. "Evaluation of Bradykinin, VEGF, and EGF Biomarkers in Gingival Crevicular Fluid and Comparison of PhotoBioModulation With Conventional Techniques in Periodontitis: a Split-mouth Randomized Clinical Trial." Lasers in Medical Science, 2019.
Angiero F, Ugolini A, Cattoni F, et al. Evaluation of bradykinin, VEGF, and EGF biomarkers in gingival crevicular fluid and comparison of PhotoBioModulation with conventional techniques in periodontitis: a split-mouth randomized clinical trial. Lasers Med Sci. 2019.
Angiero, F., Ugolini, A., Cattoni, F., Bova, F., Blasi, S., Gallo, F., ... Gherlone, E. (2019). Evaluation of bradykinin, VEGF, and EGF biomarkers in gingival crevicular fluid and comparison of PhotoBioModulation with conventional techniques in periodontitis: a split-mouth randomized clinical trial. Lasers in Medical Science, doi:10.1007/s10103-019-02919-w.
Angiero F, et al. Evaluation of Bradykinin, VEGF, and EGF Biomarkers in Gingival Crevicular Fluid and Comparison of PhotoBioModulation With Conventional Techniques in Periodontitis: a Split-mouth Randomized Clinical Trial. Lasers Med Sci. 2019 Nov 29; PubMed PMID: 31784929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of bradykinin, VEGF, and EGF biomarkers in gingival crevicular fluid and comparison of PhotoBioModulation with conventional techniques in periodontitis: a split-mouth randomized clinical trial. AU - Angiero,Francesca, AU - Ugolini,Alessandro, AU - Cattoni,Francesca, AU - Bova,Francesco, AU - Blasi,Sergio, AU - Gallo,Fabio, AU - Cossellu,Gianguido, AU - Gherlone,Enrico, Y1 - 2019/11/29/ PY - 2019/03/12/received PY - 2019/11/12/accepted PY - 2019/12/1/entrez PY - 2019/12/1/pubmed PY - 2019/12/1/medline KW - Dentistry KW - Diode KW - Effect of Lasers in Tissue KW - Lasers KW - Lasers in Dentistry KW - Low-level laser therapy KW - Periodontology KW - PhotoBioModulation KW - Wound healing JF - Lasers in medical science JO - Lasers Med Sci N2 - Periodontal disease is a chronic progressive inflammatory process leading to damage of tooth-supporting tissues. This comparative study assessed the effect of PhotoBioModulation (PBM) versus conventional therapy, and investigated biomarkers involved in the healing process. The test group comprised twenty systemically-healthy non-smoking subjects with chronic periodontitis with the presence of two matched contro-lateral premolar sites (probing depth > 5 mm); twenty subjects without chronic periodontitis (CP) served as control group. Patients were treated at baseline, either with scaling and root planing (SRP group) or with a procedure entailing SRP supported by PBM (PBM group). The laser used was a diode laser operating at 645 nm wavelength, 10 J/cm2, and 0.5 W/cm2 with a 600 μm fiber optic. Crevicular fluid levels of bradykinin (BK), vascular endothelial growth factor (VEGF), and epidermal growth factor z (EGF) were determined at both sites. Crevicular fluid specimens from both groups were analyzed with the ELISA TEST. Clinical differences in analyzed outcomes were observed in favor of PBM treatment. Taking average values as 100%, the reduction in BK concentration was 47.68% with SRP and 68.43% with PBM on day 3; the VEGF concentration decreased by 35.73% with SRP and 48.59% with PBM on day 7; the EGF concentration increased by 55.58% with SRP and by 58.11% with PBM on day 21.Clinical parameters improved significantly in both groups (pooled mean values of probing depth decreased from 5.6 to 4.5 mm; gingival index from 1.92 to 1.1; and bleeding on probing from 49.67 to 23.23) but did not vary significantly between the PBM and the SRP group. The results confirmed that PBM have beneficial effects in the early phases of the healing process playing a role in modulation of BK, EGF, and VEGF in gingival crevicular fluid levels; both groups had significant clinical improvement over control but there was no significant difference between them, only a trend for PBM group. The overall results of the study suggest a potential benefit of PBM in conjunction with SRP in treating chronic periodontitis. SN - 1435-604X UR - https://www.unboundmedicine.com/medline/citation/31784929/Evaluation_of_bradykinin,_VEGF,_and_EGF_biomarkers_in_gingival_crevicular_fluid_and_comparison_of_PhotoBioModulation_with_conventional_techniques_in_periodontitis:_a_split-mouth_randomized_clinical_trial L2 - https://dx.doi.org/10.1007/s10103-019-02919-w DB - PRIME DP - Unbound Medicine ER -