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Serotype distribution and antimicrobial resistance of human Salmonella enterica in Bangui, Central African Republic, from 2004 to 2013.
PLoS Negl Trop Dis 2019; 13(12):e0007917PN

Abstract

BACKGROUND

Limited epidemiological and antimicrobial resistance data are available on Salmonella enterica from sub-Saharan Africa. We determine the prevalence of resistance to antibiotics in isolates in the Central African Republic (CAR) between 2004 and 2013 and the genetic basis for resistance to third-generation cephalosporin (C3G).

METHODOLOGY/PRINCIPAL FINDINGS

A total of 582 non-duplicate human clinical isolates were collected. The most common serotype was Typhimurium (n = 180, 31% of the isolates). A randomly selected subset of S. Typhimurium isolates were subtyped by clustered regularly interspaced short palindromic repeat polymorphism (CRISPOL) typing. All but one invasive isolate tested (66/68, 96%) were associated with sequence type 313. Overall, the rates of resistance were high to traditional first-line drugs (18-40%) but low to many other antimicrobials, including fluoroquinolones (one resistant isolate) and C3G (only one ESBL-producing isolate). The extended-spectrum beta-lactamase (ESBL)-producing isolate and three additional ESBL isolates from West Africa were studied by whole genome sequencing. The blaCTX-M-15 gene and the majority of antimicrobial resistance genes found in the ESBL isolate were present in a large conjugative IncHI2 plasmid highly similar (> 99% nucleotide identity) to ESBL-carrying plasmids found in Kenya (S. Typhimurium ST313) and also in West Africa (serotypes Grumpensis, Havana, Telelkebir and Typhimurium).

CONCLUSIONS/SIGNIFICANCE

Although the prevalence of ESBL-producing Salmonella isolates was low in CAR, we found that a single IncHI2 plasmid-carrying blaCTX-M-15 was widespread among Salmonella serotypes from sub-Saharan Africa, which is of concern.

Authors+Show Affiliations

Laboratoire de Bactériologie, Institut Pasteur, Bangui, Central African Republic. Unité Transmission, Réservoir et Diversité des Pathogènes, Institut Pasteur de Guadeloupe, Les Abymes, France. Faculté de Médecine Hyacinthe Bastaraud, Université des Antilles, Pointe-à-Pitre, France. Laboratoire de Microbiologie clinique et environnementale, Centre Hospitalier Universitaire de Pointe-à-Pitre/les Abymes, Pointe-à-Pitre, France.Unité Transmission, Réservoir et Diversité des Pathogènes, Institut Pasteur de Guadeloupe, Les Abymes, France.Laboratoire de Bactériologie, Institut Pasteur, Bangui, Central African Republic.Laboratoire de Bactériologie, Institut Pasteur, Bangui, Central African Republic.Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Escherichia coli, Shigella et Salmonella, World Health Organization Collaborative Centre for typing and antibiotic resistance of Salmonella, Institut Pasteur, Paris, France.Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Escherichia coli, Shigella et Salmonella, World Health Organization Collaborative Centre for typing and antibiotic resistance of Salmonella, Institut Pasteur, Paris, France.Unité des Bactéries Pathogènes Entériques, Centre National de Référence des Escherichia coli, Shigella et Salmonella, World Health Organization Collaborative Centre for typing and antibiotic resistance of Salmonella, Institut Pasteur, Paris, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31790418

Citation

Breurec, Sebastien, et al. "Serotype Distribution and Antimicrobial Resistance of Human Salmonella Enterica in Bangui, Central African Republic, From 2004 to 2013." PLoS Neglected Tropical Diseases, vol. 13, no. 12, 2019, pp. e0007917.
Breurec S, Reynaud Y, Frank T, et al. Serotype distribution and antimicrobial resistance of human Salmonella enterica in Bangui, Central African Republic, from 2004 to 2013. PLoS Negl Trop Dis. 2019;13(12):e0007917.
Breurec, S., Reynaud, Y., Frank, T., Farra, A., Costilhes, G., Weill, F. X., & Le Hello, S. (2019). Serotype distribution and antimicrobial resistance of human Salmonella enterica in Bangui, Central African Republic, from 2004 to 2013. PLoS Neglected Tropical Diseases, 13(12), pp. e0007917. doi:10.1371/journal.pntd.0007917.
Breurec S, et al. Serotype Distribution and Antimicrobial Resistance of Human Salmonella Enterica in Bangui, Central African Republic, From 2004 to 2013. PLoS Negl Trop Dis. 2019;13(12):e0007917. PubMed PMID: 31790418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotype distribution and antimicrobial resistance of human Salmonella enterica in Bangui, Central African Republic, from 2004 to 2013. AU - Breurec,Sebastien, AU - Reynaud,Yann, AU - Frank,Thierry, AU - Farra,Alain, AU - Costilhes,Geoffrey, AU - Weill,François-Xavier, AU - Le Hello,Simon, Y1 - 2019/12/02/ PY - 2019/06/12/received PY - 2019/11/11/accepted PY - 2019/12/12/revised PY - 2019/12/4/pubmed PY - 2019/12/4/medline PY - 2019/12/3/entrez SP - e0007917 EP - e0007917 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 13 IS - 12 N2 - BACKGROUND: Limited epidemiological and antimicrobial resistance data are available on Salmonella enterica from sub-Saharan Africa. We determine the prevalence of resistance to antibiotics in isolates in the Central African Republic (CAR) between 2004 and 2013 and the genetic basis for resistance to third-generation cephalosporin (C3G). METHODOLOGY/PRINCIPAL FINDINGS: A total of 582 non-duplicate human clinical isolates were collected. The most common serotype was Typhimurium (n = 180, 31% of the isolates). A randomly selected subset of S. Typhimurium isolates were subtyped by clustered regularly interspaced short palindromic repeat polymorphism (CRISPOL) typing. All but one invasive isolate tested (66/68, 96%) were associated with sequence type 313. Overall, the rates of resistance were high to traditional first-line drugs (18-40%) but low to many other antimicrobials, including fluoroquinolones (one resistant isolate) and C3G (only one ESBL-producing isolate). The extended-spectrum beta-lactamase (ESBL)-producing isolate and three additional ESBL isolates from West Africa were studied by whole genome sequencing. The blaCTX-M-15 gene and the majority of antimicrobial resistance genes found in the ESBL isolate were present in a large conjugative IncHI2 plasmid highly similar (> 99% nucleotide identity) to ESBL-carrying plasmids found in Kenya (S. Typhimurium ST313) and also in West Africa (serotypes Grumpensis, Havana, Telelkebir and Typhimurium). CONCLUSIONS/SIGNIFICANCE: Although the prevalence of ESBL-producing Salmonella isolates was low in CAR, we found that a single IncHI2 plasmid-carrying blaCTX-M-15 was widespread among Salmonella serotypes from sub-Saharan Africa, which is of concern. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/31790418/Serotype_distribution_and_antimicrobial_resistance_of_human_Salmonella_enterica_in_Bangui,_Central_African_Republic,_from_2004_to_2013 L2 - http://dx.plos.org/10.1371/journal.pntd.0007917 DB - PRIME DP - Unbound Medicine ER -