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Forensic genetic investigation of human skeletal remains recovered from the La Belle shipwreck.
Forensic Sci Int. 2020 Jan; 306:110050.FS

Abstract

In 1995, the historical shipwreck of La Belle was discovered off the coast of Texas. One partial human skeleton was recovered from alongside cargo in the rear portion of the ship; a second (complete) skeleton was found atop coiled anchor rope in the bow. In late 2015, comprehensive forensic genetic testing began on multiple samplings from each set of remains. For the partial skeleton recovered from the ship's rear cargo area, results were obtained for 26/27 Y-STRs using traditional CE; with MPS technology, results were obtained for 18/24 Y-STRs, 56/56 ancestry-informative SNPs (aiSNPs), 22/22 phenotype-informative SNPs (piSNPs), 22/27 autosomal STRs, 4/7 X-STRs, and 94/94 identity-informative SNPs (iiSNPs). For the complete skeleton of the second individual, results were obtained for 7/17 Y-STRs using traditional CE; with MPS technology, results were obtained for 5/24 Y-STRs, 49/56 aiSNPs, 18/22 piSNPs, 15/27 autosomal STRs, 1/7 X-STRs, and 66/94 iiSNPs. Biogeographic ancestry for each set of skeletal remains was predicted using the ancestry feature and metapopulation tool of the Y-STR Haplotype Reference Database (YHRD), Haplogroup Predictor, and the Forensic Research/Reference on Genetics knowledge base (FROG-kb). Phenotype prediction was performed using piSNP data and the HIrisplex eye color and hair color DNA phenotyping webtool. mtDNA whole genome sequencing also was performed successfully. This study highlights the sensitivity of current forensic laboratory methods in recovering DNA from historical and archaeological human remains. Using advanced sequencing technology provided by MiSeq™ FGx (Verogen) and Ion S5™ (Thermo Fisher Scientific) instrumentation, degraded skeletal remains can be characterized using a panel of diverse and highly informative markers, producing data which can be useful in both forensic and genealogical investigations.

Authors+Show Affiliations

Center for Human Identification, Research and Development Unit, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Forensic Science Department, Henry C. Lee College of Criminal Justice and Forensic Sciences, University of New Haven, 300 Boston Post Road, West Haven, CT 06516, USA. Electronic address: aambers@newhaven.edu.Center for Human Identification, Research and Development Unit, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.Center for Human Identification, Research and Development Unit, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.Texas Historical Commission, Archaeology Division, P.O. Box 12276, Austin, TX 78711, USA.Texas Historical Commission, Archaeology Division, P.O. Box 12276, Austin, TX 78711, USA.Texas Historical Commission, Archaeology Division, P.O. Box 12276, Austin, TX 78711, USA.Center for Human Identification, Forensic Anthropology Unit, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Institute of Forensic Anthropology and Human Evolution, Department of Biological Sciences, University of North Texas, 1510 Chestnut Street, Denton, TX 76201, USA.Center for Human Identification, Research and Development Unit, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA; Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA.

Pub Type(s)

Historical Article
Journal Article

Language

eng

PubMed ID

31790892

Citation

Ambers, Angie, et al. "Forensic Genetic Investigation of Human Skeletal Remains Recovered From the La Belle Shipwreck." Forensic Science International, vol. 306, 2020, p. 110050.
Ambers A, Bus MM, King JL, et al. Forensic genetic investigation of human skeletal remains recovered from the La Belle shipwreck. Forensic Sci Int. 2020;306:110050.
Ambers, A., Bus, M. M., King, J. L., Jones, B., Durst, J., Bruseth, J. E., Gill-King, H., & Budowle, B. (2020). Forensic genetic investigation of human skeletal remains recovered from the La Belle shipwreck. Forensic Science International, 306, 110050. https://doi.org/10.1016/j.forsciint.2019.110050
Ambers A, et al. Forensic Genetic Investigation of Human Skeletal Remains Recovered From the La Belle Shipwreck. Forensic Sci Int. 2020;306:110050. PubMed PMID: 31790892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Forensic genetic investigation of human skeletal remains recovered from the La Belle shipwreck. AU - Ambers,Angie, AU - Bus,Magdalena M, AU - King,Jonathan L, AU - Jones,Bradford, AU - Durst,Jeffrey, AU - Bruseth,James E, AU - Gill-King,Harrell, AU - Budowle,Bruce, Y1 - 2019/11/12/ PY - 2019/08/12/received PY - 2019/10/10/revised PY - 2019/11/08/accepted PY - 2019/12/4/pubmed PY - 2020/1/29/medline PY - 2019/12/3/entrez KW - FROG-kb KW - ForenSeq™ KW - La Belle KW - MPS KW - Precision ID mtDNA whole genome panel KW - Robert Cavalier KW - Sieur de La Salle KW - X-STR KW - Y-STR KW - Yfiler™ Plus KW - ancestry SNP KW - biogeographic ancestry KW - lineage KW - massively parallel sequencing KW - mtDNA KW - phenotype SNP KW - skeletal remains SP - 110050 EP - 110050 JF - Forensic science international JO - Forensic Sci Int VL - 306 N2 - In 1995, the historical shipwreck of La Belle was discovered off the coast of Texas. One partial human skeleton was recovered from alongside cargo in the rear portion of the ship; a second (complete) skeleton was found atop coiled anchor rope in the bow. In late 2015, comprehensive forensic genetic testing began on multiple samplings from each set of remains. For the partial skeleton recovered from the ship's rear cargo area, results were obtained for 26/27 Y-STRs using traditional CE; with MPS technology, results were obtained for 18/24 Y-STRs, 56/56 ancestry-informative SNPs (aiSNPs), 22/22 phenotype-informative SNPs (piSNPs), 22/27 autosomal STRs, 4/7 X-STRs, and 94/94 identity-informative SNPs (iiSNPs). For the complete skeleton of the second individual, results were obtained for 7/17 Y-STRs using traditional CE; with MPS technology, results were obtained for 5/24 Y-STRs, 49/56 aiSNPs, 18/22 piSNPs, 15/27 autosomal STRs, 1/7 X-STRs, and 66/94 iiSNPs. Biogeographic ancestry for each set of skeletal remains was predicted using the ancestry feature and metapopulation tool of the Y-STR Haplotype Reference Database (YHRD), Haplogroup Predictor, and the Forensic Research/Reference on Genetics knowledge base (FROG-kb). Phenotype prediction was performed using piSNP data and the HIrisplex eye color and hair color DNA phenotyping webtool. mtDNA whole genome sequencing also was performed successfully. This study highlights the sensitivity of current forensic laboratory methods in recovering DNA from historical and archaeological human remains. Using advanced sequencing technology provided by MiSeq™ FGx (Verogen) and Ion S5™ (Thermo Fisher Scientific) instrumentation, degraded skeletal remains can be characterized using a panel of diverse and highly informative markers, producing data which can be useful in both forensic and genealogical investigations. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/31790892/Forensic_genetic_investigation_of_human_skeletal_remains_recovered_from_the_La_Belle_shipwreck_ DB - PRIME DP - Unbound Medicine ER -