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The complex of Plasmodium falciparum falcipain-2 protease with an (E)-chalcone-based inhibitor highlights a novel, small, molecule-binding site.
Malar J 2019; 18(1):388MJ

Abstract

BACKGROUND

Malaria kills over 400,000 people each year and nearly half the world's population live in at-risk areas. Progress against malaria has recently stalled, highlighting the need for developing novel therapeutics. The parasite haemoglobin degradation pathway, active in the blood stage of the disease where malaria symptoms and lethality manifest, is a well-established drug target. A key enzyme in this pathway is the papain-type protease falcipain-2.

METHODS

The crystallographic structure of falcipain-2 at 3.45 Å resolution was resolved in complex with an (E)-chalcone small-molecule inhibitor. The falcipain-2-(E)-chalcone complex was analysed with reference to previous falcipain complexes and their similarity to human cathepsin proteases.

RESULTS

The (E)-chalcone inhibitor binds falcipain-2 to the rear of the substrate-binding cleft. This is the first structure of a falcipain protease where the rear of the substrate cleft is bound by a small molecule. In this manner, the (E)-chalcone inhibitor mimics interactions observed in protein-based falcipain inhibitors, which can achieve high interaction specificity.

CONCLUSIONS

This work informs the search for novel anti-malaria therapeutics that target falcipain-2 by showing the binding site and interactions of the medically privileged (E)-chalcone molecule. Furthermore, this study highlights the possibility of chemically combining the (E)-chalcone molecule with an existing active-site inhibitor of falcipain, which may yield a potent and selective compound for blocking haemoglobin degradation by the malaria parasite.

Authors+Show Affiliations

Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK. ioannis.vakonakis@bioch.ox.ac.uk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31791339

Citation

Machin, Jonathan M., et al. "The Complex of Plasmodium Falciparum Falcipain-2 Protease With an (E)-chalcone-based Inhibitor Highlights a Novel, Small, Molecule-binding Site." Malaria Journal, vol. 18, no. 1, 2019, p. 388.
Machin JM, Kantsadi AL, Vakonakis I. The complex of Plasmodium falciparum falcipain-2 protease with an (E)-chalcone-based inhibitor highlights a novel, small, molecule-binding site. Malar J. 2019;18(1):388.
Machin, J. M., Kantsadi, A. L., & Vakonakis, I. (2019). The complex of Plasmodium falciparum falcipain-2 protease with an (E)-chalcone-based inhibitor highlights a novel, small, molecule-binding site. Malaria Journal, 18(1), p. 388. doi:10.1186/s12936-019-3043-0.
Machin JM, Kantsadi AL, Vakonakis I. The Complex of Plasmodium Falciparum Falcipain-2 Protease With an (E)-chalcone-based Inhibitor Highlights a Novel, Small, Molecule-binding Site. Malar J. 2019 Dec 2;18(1):388. PubMed PMID: 31791339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The complex of Plasmodium falciparum falcipain-2 protease with an (E)-chalcone-based inhibitor highlights a novel, small, molecule-binding site. AU - Machin,Jonathan M, AU - Kantsadi,Anastassia L, AU - Vakonakis,Ioannis, Y1 - 2019/12/02/ PY - 2019/09/28/received PY - 2019/11/27/accepted PY - 2019/12/4/entrez PY - 2019/12/4/pubmed PY - 2019/12/4/medline KW - Complex KW - Falcipain KW - Inhibitor KW - Plasmodium KW - Structure KW - X-ray crystallography SP - 388 EP - 388 JF - Malaria journal JO - Malar. J. VL - 18 IS - 1 N2 - BACKGROUND: Malaria kills over 400,000 people each year and nearly half the world's population live in at-risk areas. Progress against malaria has recently stalled, highlighting the need for developing novel therapeutics. The parasite haemoglobin degradation pathway, active in the blood stage of the disease where malaria symptoms and lethality manifest, is a well-established drug target. A key enzyme in this pathway is the papain-type protease falcipain-2. METHODS: The crystallographic structure of falcipain-2 at 3.45 Å resolution was resolved in complex with an (E)-chalcone small-molecule inhibitor. The falcipain-2-(E)-chalcone complex was analysed with reference to previous falcipain complexes and their similarity to human cathepsin proteases. RESULTS: The (E)-chalcone inhibitor binds falcipain-2 to the rear of the substrate-binding cleft. This is the first structure of a falcipain protease where the rear of the substrate cleft is bound by a small molecule. In this manner, the (E)-chalcone inhibitor mimics interactions observed in protein-based falcipain inhibitors, which can achieve high interaction specificity. CONCLUSIONS: This work informs the search for novel anti-malaria therapeutics that target falcipain-2 by showing the binding site and interactions of the medically privileged (E)-chalcone molecule. Furthermore, this study highlights the possibility of chemically combining the (E)-chalcone molecule with an existing active-site inhibitor of falcipain, which may yield a potent and selective compound for blocking haemoglobin degradation by the malaria parasite. SN - 1475-2875 UR - https://www.unboundmedicine.com/medline/citation/31791339/The_complex_of_Plasmodium_falciparum_falcipain-2_protease_with_an_(E)-chalcone-based_inhibitor_highlights_a_novel,_small,_molecule-binding_site L2 - https://malariajournal.biomedcentral.com/articles/10.1186/s12936-019-3043-0 DB - PRIME DP - Unbound Medicine ER -