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Middle East respiratory syndrome coronavirus (MERS-CoV) neutralising antibodies in a high-risk human population, Morocco, November 2017 to January 2018.
Euro Surveill. 2019 Nov; 24(48)ES

Abstract

BackgroundMiddle East respiratory syndrome coronavirus (MERS-CoV) remains a major concern for global public health. Dromedaries are the source of human zoonotic infection. MERS-CoV is enzootic among dromedaries on the Arabian Peninsula, the Middle East and in Africa. Over 70% of infected dromedaries are found in Africa. However, all known zoonotic cases of MERS have occurred in the Arabian Peninsula with none being reported in Africa.AimWe aimed to investigate serological evidence of MERS-CoV infection in humans living in camel-herding areas in Morocco to provide insights on whether zoonotic transmission is taking place.MethodsWe carried out a cross sectional seroprevalence study from November 2017 through January 2018. We adapted a generic World Health Organization MERS-CoV questionnaire and protocol to assess demographic and risk factors of infection among a presumed high-risk population. ELISA, MERS-CoV spike pseudoparticle neutralisation tests (ppNT) and plaque neutralisation tests (PRNT) were used to assess MERS-CoV seropositivity.ResultsSerum samples were collected from camel slaughterhouse workers (n = 137), camel herders (n = 156) and individuals of the general population without occupational contact with camels but living in camel herding areas (n = 186). MERS-CoV neutralising antibodies with ≥ 90% reduction of plaque numbers were detected in two (1.5%) slaughterhouse workers, none of the camel herders and one individual from the general population (0.5%).ConclusionsThis study provides evidence of zoonotic transmission of MERS-CoV in Morocco in people who have direct or indirect exposure to dromedary camels.

Authors+Show Affiliations

These authors contributed equally to this work. Laboratoire d'Ecologie et d'Environnement, Faculté des Sciences Ben M'Sik, Université Hassan II, Casablanca, Morocco. Medical Virology and BSL-3 Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.These authors contributed equally to this work. School of Public Health, University of Hong-Kong, Hong Kong SAR, China.Medical Virology and BSL-3 Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.Medical Virology and BSL-3 Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.Health Emergencies Programme, World Health Organization, Regional Office for the Eastern Mediterranean, Cairo, Egypt.Health Emergencies Programme, World Health Organization, Regional Office for the Eastern Mediterranean, Cairo, Egypt.Laboratoire d'Ecologie et d'Environnement, Faculté des Sciences Ben M'Sik, Université Hassan II, Casablanca, Morocco.Medical Virology and BSL-3 Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.Health Emergencies Programme, World Health Organization, Geneva, Switzerland.HKU-Pasteur Research Pole, University of Hong Kong, Hong Kong SAR, China. School of Public Health, University of Hong-Kong, Hong Kong SAR, China.Medical Virology and BSL-3 Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31796154

Citation

Abbad, Anass, et al. "Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Neutralising Antibodies in a High-risk Human Population, Morocco, November 2017 to January 2018." Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, vol. 24, no. 48, 2019.
Abbad A, Perera RA, Anga L, et al. Middle East respiratory syndrome coronavirus (MERS-CoV) neutralising antibodies in a high-risk human population, Morocco, November 2017 to January 2018. Euro Surveill. 2019;24(48).
Abbad, A., Perera, R. A., Anga, L., Faouzi, A., Minh, N. N. T., Malik, S. M. M. R., Iounes, N., Maaroufi, A., Van Kerkhove, M. D., Peiris, M., & Nourlil, J. (2019). Middle East respiratory syndrome coronavirus (MERS-CoV) neutralising antibodies in a high-risk human population, Morocco, November 2017 to January 2018. Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, 24(48). https://doi.org/10.2807/1560-7917.ES.2019.24.48.1900244
Abbad A, et al. Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Neutralising Antibodies in a High-risk Human Population, Morocco, November 2017 to January 2018. Euro Surveill. 2019;24(48) PubMed PMID: 31796154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Middle East respiratory syndrome coronavirus (MERS-CoV) neutralising antibodies in a high-risk human population, Morocco, November 2017 to January 2018. AU - Abbad,Anass, AU - Perera,Ranawaka Apm, AU - Anga,Latifa, AU - Faouzi,Abdellah, AU - Minh,Nhu Nguyen Tran, AU - Malik,Sk Md Mamunur Rahman, AU - Iounes,Nadia, AU - Maaroufi,Abderrahmane, AU - Van Kerkhove,Maria D, AU - Peiris,Malik, AU - Nourlil,Jalal, PY - 2019/12/5/entrez PY - 2019/12/5/pubmed PY - 2020/8/6/medline KW - MERS-CoV KW - Middle East Respiratory Syndrome Coronavirus KW - Morocco KW - dromedaries KW - transmission KW - zoonosis JF - Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin JO - Euro Surveill VL - 24 IS - 48 N2 - BackgroundMiddle East respiratory syndrome coronavirus (MERS-CoV) remains a major concern for global public health. Dromedaries are the source of human zoonotic infection. MERS-CoV is enzootic among dromedaries on the Arabian Peninsula, the Middle East and in Africa. Over 70% of infected dromedaries are found in Africa. However, all known zoonotic cases of MERS have occurred in the Arabian Peninsula with none being reported in Africa.AimWe aimed to investigate serological evidence of MERS-CoV infection in humans living in camel-herding areas in Morocco to provide insights on whether zoonotic transmission is taking place.MethodsWe carried out a cross sectional seroprevalence study from November 2017 through January 2018. We adapted a generic World Health Organization MERS-CoV questionnaire and protocol to assess demographic and risk factors of infection among a presumed high-risk population. ELISA, MERS-CoV spike pseudoparticle neutralisation tests (ppNT) and plaque neutralisation tests (PRNT) were used to assess MERS-CoV seropositivity.ResultsSerum samples were collected from camel slaughterhouse workers (n = 137), camel herders (n = 156) and individuals of the general population without occupational contact with camels but living in camel herding areas (n = 186). MERS-CoV neutralising antibodies with ≥ 90% reduction of plaque numbers were detected in two (1.5%) slaughterhouse workers, none of the camel herders and one individual from the general population (0.5%).ConclusionsThis study provides evidence of zoonotic transmission of MERS-CoV in Morocco in people who have direct or indirect exposure to dromedary camels. SN - 1560-7917 UR - https://www.unboundmedicine.com/medline/citation/31796154/Middle_East_respiratory_syndrome_coronavirus__MERS_CoV__neutralising_antibodies_in_a_high_risk_human_population_Morocco_November_2017_to_January_2018_ L2 - http://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2019.24.48.1900244 DB - PRIME DP - Unbound Medicine ER -