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Simple and sensitive method for analysis of 14 anti-tuberculosis drugs using LC/MS/MS in human plasma.

Abstract

RATIONALE

The monitoring of the plasma concentration and dose adjustment of anti-tuberculosis (TB) drugs are beneficial for improving responses to drug treatment and avoiding drug adverse reactions. A simple and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was developed to measure the plasma concentrations of 14 anti-TB drugs: ethambutol, isoniazid, pyrazinamide, levofloxacin, gatifloxacin, moxifloxacin, prothionamide, linezolid, rifampin, rifapentine, rifabutin, cycloserine, p-aminosalicylic acid (PAS) and clofazimine.

METHODS

Human plasma was precipitated by acetonitrile and was subsequently separated by an AQ-C18 column with a gradient elution. Drug concentrations were determined using multiple reaction monitoring (MRM) in positive ion ESI mode. According to pharmacokinetic data of patients, the peak concentration ranges and timing of blood collection were determined.

RESULTS

Intra- and inter-day precision was less than 14.8%. Linearity, accuracy, extraction recovery and matrix effect were acceptable for each drug. Stability of the method satisfied different storage conditions.

CONCLUSIONS

The method applied the sensitive and reproducible determination of 14 frequently-used anti-TB drugs, which already showed benefit for some TB patients.

Authors+Show Affiliations

Department of Pharmacy.Department of Pharmacy.Office of Drug Clinical Trial Institution.Department of Pharmacy.Department of Pharmacy.Department of Medical, Xi'an Chest Hospital, Xi'an, Shaanxi, China.Department of Medical, Xi'an Chest Hospital, Xi'an, Shaanxi, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31800129

Citation

Lei, Qian, et al. "Simple and Sensitive Method for Analysis of 14 Anti-tuberculosis Drugs Using LC/MS/MS in Human Plasma." Rapid Communications in Mass Spectrometry : RCM, 2019.
Lei Q, Zhao Y, Wang H, et al. Simple and sensitive method for analysis of 14 anti-tuberculosis drugs using LC/MS/MS in human plasma. Rapid Commun Mass Spectrom. 2019.
Lei, Q., Zhao, Y., Wang, H., Zhou, J., Lv, X., Dang, L., & Zhu, C. (2019). Simple and sensitive method for analysis of 14 anti-tuberculosis drugs using LC/MS/MS in human plasma. Rapid Communications in Mass Spectrometry : RCM, doi:10.1002/rcm.8667.
Lei Q, et al. Simple and Sensitive Method for Analysis of 14 Anti-tuberculosis Drugs Using LC/MS/MS in Human Plasma. Rapid Commun Mass Spectrom. 2019 Dec 4; PubMed PMID: 31800129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple and sensitive method for analysis of 14 anti-tuberculosis drugs using LC/MS/MS in human plasma. AU - Lei,Qian, AU - Zhao,Yuan, AU - Wang,Hao, AU - Zhou,Jun, AU - Lv,Xiaohui, AU - Dang,Liyun, AU - Zhu,Changsheng, Y1 - 2019/12/04/ PY - 2019/09/23/received PY - 2019/11/19/revised PY - 2019/11/20/accepted PY - 2019/12/5/entrez PY - 2019/12/5/pubmed PY - 2019/12/5/medline JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun. Mass Spectrom. N2 - RATIONALE: The monitoring of the plasma concentration and dose adjustment of anti-tuberculosis (TB) drugs are beneficial for improving responses to drug treatment and avoiding drug adverse reactions. A simple and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was developed to measure the plasma concentrations of 14 anti-TB drugs: ethambutol, isoniazid, pyrazinamide, levofloxacin, gatifloxacin, moxifloxacin, prothionamide, linezolid, rifampin, rifapentine, rifabutin, cycloserine, p-aminosalicylic acid (PAS) and clofazimine. METHODS: Human plasma was precipitated by acetonitrile and was subsequently separated by an AQ-C18 column with a gradient elution. Drug concentrations were determined using multiple reaction monitoring (MRM) in positive ion ESI mode. According to pharmacokinetic data of patients, the peak concentration ranges and timing of blood collection were determined. RESULTS: Intra- and inter-day precision was less than 14.8%. Linearity, accuracy, extraction recovery and matrix effect were acceptable for each drug. Stability of the method satisfied different storage conditions. CONCLUSIONS: The method applied the sensitive and reproducible determination of 14 frequently-used anti-TB drugs, which already showed benefit for some TB patients. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/31800129/Simple_and_sensitive_method_for_analysis_of_14_anti-tuberculosis_drugs_using_LC/MS/MS_in_human_plasma L2 - https://doi.org/10.1002/rcm.8667 DB - PRIME DP - Unbound Medicine ER -