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Straining 3D Hydrogels with Uniform Z-Axis Strains While Enabling Live Microscopy Imaging.
Ann Biomed Eng. 2020 Feb; 48(2):868-880.AB

Abstract

External forces play an important role in the development and regulation of many tissues. Such effects are often studied using specialized stretchers-standardized commercial and novel laboratory-designed. While designs for 2D stretchers are abundant, the range of available 3D stretcher designs is more limited, especially when live imaging is required. This work presents a novel method and a stretching device that allow straining of 3D hydrogels from their circumference, using a punctured elastic silicone strip as the sample carrier. The system was primarily constructed from 3D-printed parts and low-cost electronics, rendering it simple and cost-efficient to reproduce in other labs. To demonstrate the system functionality, > 100 μm thick soft fibrin gels (< 1 KPa) were stretched, while performing live confocal imaging. The subsequent strains and fiber alignment were analyzed and found to be relatively homogenous throughout the gel's thickness (Z axis). The uniform Z-response enabled by our approach was found to be in contrast to a previously reported approach that utilizes an underlying elastic substrate to convey strain to a 3D thick sample. This work advances the ability to study the role of external forces on biological processes under more physiological 3D conditions, and can contribute to the field of tissue engineering.

Authors+Show Affiliations

School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel.School of Mechanical Engineering, Faculty of Engineering, Tel-Aviv University, Tel-Aviv, Israel. ayeletlesman@tauex.tau.ac.il.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31802281

Citation

Roitblat Riba, Avishy, et al. "Straining 3D Hydrogels With Uniform Z-Axis Strains While Enabling Live Microscopy Imaging." Annals of Biomedical Engineering, vol. 48, no. 2, 2020, pp. 868-880.
Roitblat Riba A, Natan S, Kolel A, et al. Straining 3D Hydrogels with Uniform Z-Axis Strains While Enabling Live Microscopy Imaging. Ann Biomed Eng. 2020;48(2):868-880.
Roitblat Riba, A., Natan, S., Kolel, A., Rushkin, H., Tchaicheeyan, O., & Lesman, A. (2020). Straining 3D Hydrogels with Uniform Z-Axis Strains While Enabling Live Microscopy Imaging. Annals of Biomedical Engineering, 48(2), 868-880. https://doi.org/10.1007/s10439-019-02426-7
Roitblat Riba A, et al. Straining 3D Hydrogels With Uniform Z-Axis Strains While Enabling Live Microscopy Imaging. Ann Biomed Eng. 2020;48(2):868-880. PubMed PMID: 31802281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Straining 3D Hydrogels with Uniform Z-Axis Strains While Enabling Live Microscopy Imaging. AU - Roitblat Riba,Avishy, AU - Natan,Sari, AU - Kolel,Avraham, AU - Rushkin,Hila, AU - Tchaicheeyan,Oren, AU - Lesman,Ayelet, Y1 - 2019/12/04/ PY - 2019/08/11/received PY - 2019/11/24/accepted PY - 2019/12/6/pubmed PY - 2019/12/6/medline PY - 2019/12/6/entrez KW - Cell mechanics KW - External stretching KW - Extracellular matrix KW - Fiber alignment KW - Fibrous network KW - Hydrogel KW - Mechanical force KW - Mechanobiology SP - 868 EP - 880 JF - Annals of biomedical engineering JO - Ann Biomed Eng VL - 48 IS - 2 N2 - External forces play an important role in the development and regulation of many tissues. Such effects are often studied using specialized stretchers-standardized commercial and novel laboratory-designed. While designs for 2D stretchers are abundant, the range of available 3D stretcher designs is more limited, especially when live imaging is required. This work presents a novel method and a stretching device that allow straining of 3D hydrogels from their circumference, using a punctured elastic silicone strip as the sample carrier. The system was primarily constructed from 3D-printed parts and low-cost electronics, rendering it simple and cost-efficient to reproduce in other labs. To demonstrate the system functionality, > 100 μm thick soft fibrin gels (< 1 KPa) were stretched, while performing live confocal imaging. The subsequent strains and fiber alignment were analyzed and found to be relatively homogenous throughout the gel's thickness (Z axis). The uniform Z-response enabled by our approach was found to be in contrast to a previously reported approach that utilizes an underlying elastic substrate to convey strain to a 3D thick sample. This work advances the ability to study the role of external forces on biological processes under more physiological 3D conditions, and can contribute to the field of tissue engineering. SN - 1573-9686 UR - https://www.unboundmedicine.com/medline/citation/31802281/Straining_3D_Hydrogels_with_Uniform_Z-Axis_Strains_While_Enabling_Live_Microscopy_Imaging L2 - https://doi.org/10.1007/s10439-019-02426-7 DB - PRIME DP - Unbound Medicine ER -
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