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Effects of dietary α-linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus).
J Sci Food Agric. 2020 Mar 15; 100(4):1643-1652.JS

Abstract

BACKGROUND

Silver barb (Puntius gonionotus) is a medium-sized carp that is promising for freshwater aquaculture in Asia. This study's aim was to investigate the ideal dietary α-linolenic acid (ALA): linoleic acid (LA) ratio for maximizing long-chain polyunsaturated fatty acid (LC-PUFA) synthesis and their deposition in the muscle of silver barb, as that of fish oil based control diet.

RESULT

Fish (with an initial body weight of 11.07 ± 0.12 g) were fed for 60 days with five experimental iso-proteinous, iso-lipidic, and iso-caloric diets, supplemented with linseed oil and peanut oil at varying levels to obtain ALA:LA ratios of 0.35, 0.51, 0.91, 2.04, 2.66. A control diet was prepared by supplementing fish oil. The dietary ALA:LA ratio did not influence the growth performance of fish. With increased dietary ALA:LA ratios, LA content decreased and ALA content increased in the muscle and liver of silver barb. The n-3 LC-PUFA level in muscle and liver was not influenced by feeding different ratios of ALA:LA, whereas n-6 LC-PUFA was decreased in the muscle and increased in the liver with increased dietary ALA:LA ratios. Increasing dietary ALA:LA ratio increased the Δ6fad and elovl5mRNA expression in the liver, muscle, brain, and intestinal tissues of silver barbs.

CONCLUSION

Silver barb possess the ability to elongate and desaturate ALA and LA to their end products EPA and DHA. The highest level expression of Δ6 fad and elovl5 mRNA at the dietary ALA:LA ratio of 2.66 suggests greater affinity of these enzymes towards ALA than LA in silver barb. © 2019 Society of Chemical Industry.

Authors+Show Affiliations

Nutrigenomics Laboratory, Fish Nutrition and Physiology Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.Nutrigenomics Laboratory, Fish Nutrition and Physiology Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.Nutrigenomics Laboratory, Fish Nutrition and Physiology Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.Nutrigenomics Laboratory, Fish Nutrition and Physiology Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.Nutrigenomics Laboratory, Fish Nutrition and Physiology Division, ICAR- Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31802501

Citation

Nayak, Madhusmita, et al. "Effects of Dietary Α-linolenic Acid/linoleic Acid Ratio On Growth Performance, Tissue Fatty Acid Profile, Serum Metabolites and Δ6 Fad and Elovl5 Gene Expression in Silver Barb (Puntius Gonionotus)." Journal of the Science of Food and Agriculture, vol. 100, no. 4, 2020, pp. 1643-1652.
Nayak M, Giri SS, Pradhan A, et al. Effects of dietary α-linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus). J Sci Food Agric. 2020;100(4):1643-1652.
Nayak, M., Giri, S. S., Pradhan, A., Samanta, M., & Saha, A. (2020). Effects of dietary α-linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus). Journal of the Science of Food and Agriculture, 100(4), 1643-1652. https://doi.org/10.1002/jsfa.10177
Nayak M, et al. Effects of Dietary Α-linolenic Acid/linoleic Acid Ratio On Growth Performance, Tissue Fatty Acid Profile, Serum Metabolites and Δ6 Fad and Elovl5 Gene Expression in Silver Barb (Puntius Gonionotus). J Sci Food Agric. 2020 Mar 15;100(4):1643-1652. PubMed PMID: 31802501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary α-linolenic acid/linoleic acid ratio on growth performance, tissue fatty acid profile, serum metabolites and Δ6 fad and elovl5 gene expression in silver barb (Puntius gonionotus). AU - Nayak,Madhusmita, AU - Giri,Shiba Shankar, AU - Pradhan,Avinash, AU - Samanta,Mrinal, AU - Saha,Ashis, Y1 - 2019/12/27/ PY - 2019/09/12/received PY - 2019/11/26/revised PY - 2019/11/28/accepted PY - 2019/12/6/pubmed PY - 2020/2/14/medline PY - 2019/12/6/entrez KW - ALA KW - LA KW - desaturase KW - elongase KW - fish oil replacement KW - silver barb diet SP - 1643 EP - 1652 JF - Journal of the science of food and agriculture JO - J. Sci. Food Agric. VL - 100 IS - 4 N2 - BACKGROUND: Silver barb (Puntius gonionotus) is a medium-sized carp that is promising for freshwater aquaculture in Asia. This study's aim was to investigate the ideal dietary α-linolenic acid (ALA): linoleic acid (LA) ratio for maximizing long-chain polyunsaturated fatty acid (LC-PUFA) synthesis and their deposition in the muscle of silver barb, as that of fish oil based control diet. RESULT: Fish (with an initial body weight of 11.07 ± 0.12 g) were fed for 60 days with five experimental iso-proteinous, iso-lipidic, and iso-caloric diets, supplemented with linseed oil and peanut oil at varying levels to obtain ALA:LA ratios of 0.35, 0.51, 0.91, 2.04, 2.66. A control diet was prepared by supplementing fish oil. The dietary ALA:LA ratio did not influence the growth performance of fish. With increased dietary ALA:LA ratios, LA content decreased and ALA content increased in the muscle and liver of silver barb. The n-3 LC-PUFA level in muscle and liver was not influenced by feeding different ratios of ALA:LA, whereas n-6 LC-PUFA was decreased in the muscle and increased in the liver with increased dietary ALA:LA ratios. Increasing dietary ALA:LA ratio increased the Δ6fad and elovl5mRNA expression in the liver, muscle, brain, and intestinal tissues of silver barbs. CONCLUSION: Silver barb possess the ability to elongate and desaturate ALA and LA to their end products EPA and DHA. The highest level expression of Δ6 fad and elovl5 mRNA at the dietary ALA:LA ratio of 2.66 suggests greater affinity of these enzymes towards ALA than LA in silver barb. © 2019 Society of Chemical Industry. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/31802501/Effects_of_dietary_α_linolenic_acid/linoleic_acid_ratio_on_growth_performance_tissue_fatty_acid_profile_serum_metabolites_and_Δ6_fad_and_elovl5_gene_expression_in_silver_barb__Puntius_gonionotus__ L2 - https://doi.org/10.1002/jsfa.10177 DB - PRIME DP - Unbound Medicine ER -