Tags

Type your tag names separated by a space and hit enter

Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients' Stomachs.
Infect Drug Resist 2019; 12:3415-3420ID

Abstract

Introduction

Helicobacter pylori was discovered first in the stomachs of patients with gastritis and ulcers by Marshall and Warren in 1982. This discovery majorly affected many research areas of gastroenterology. Since then, the main aim has been to eradicate this microaerophilic bacterium from the stomachs of infected subjects.

Methods

We studied symptomatic cases by endoscopic surgery and examined the prevalence of cagA-vacA genotypes among the H. pylori isolates. H. pylori isolated from antral biopsies of patients with gastritis and duodenal ulcer were subjected to antimicrobial susceptibility testing and PCR genotyping by using routine bacterial cultures. Clarithromycin-susceptibility profiling was done by the E-test. DNA was extracted using standard manufacturer protocols with minor modifications and cagA and vacA genotyping was done PCR.

Results

In our study, all strains identified as H. pylori in culture (61/81) were confirmed by PCR by amplifying a fragment of the glmM gene. Totally, 61 patients were confirmed to be positive for H. pylori and they were included in the genotyping and antibiotic-susceptibility testing. Thirteen H. pylori strains were determined to be resistant to clarithromycin.

Discussion

Current accumulating data indicate that both clarithromycin-resistant and susceptible isolates of H. pylori need to be screened and tracked in populations.

Authors+Show Affiliations

Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31802920

Citation

Khani, Shadiyeh, et al. "Clarithromycin-Susceptible but Virulent Helicobacter Pylori Strains Infecting Iranian Patients' Stomachs." Infection and Drug Resistance, vol. 12, 2019, pp. 3415-3420.
Khani S, Talebi Bezmin Abadi A, Mohabati Mobarez A. Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients' Stomachs. Infect Drug Resist. 2019;12:3415-3420.
Khani, S., Talebi Bezmin Abadi, A., & Mohabati Mobarez, A. (2019). Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients' Stomachs. Infection and Drug Resistance, 12, pp. 3415-3420. doi:10.2147/IDR.S223602.
Khani S, Talebi Bezmin Abadi A, Mohabati Mobarez A. Clarithromycin-Susceptible but Virulent Helicobacter Pylori Strains Infecting Iranian Patients' Stomachs. Infect Drug Resist. 2019;12:3415-3420. PubMed PMID: 31802920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clarithromycin-Susceptible But Virulent Helicobacter pylori Strains Infecting Iranian Patients' Stomachs. AU - Khani,Shadiyeh, AU - Talebi Bezmin Abadi,Amin, AU - Mohabati Mobarez,Ashraf, Y1 - 2019/11/01/ PY - 2019/07/17/received PY - 2019/10/24/accepted PY - 2019/12/6/entrez PY - 2019/12/6/pubmed PY - 2019/12/6/medline KW - E-test KW - Helicobacter pylori KW - PCR KW - antibiotic susceptibility KW - cagA KW - clarithromycin KW - genotypes KW - vacA SP - 3415 EP - 3420 JF - Infection and drug resistance JO - Infect Drug Resist VL - 12 N2 - Introduction: Helicobacter pylori was discovered first in the stomachs of patients with gastritis and ulcers by Marshall and Warren in 1982. This discovery majorly affected many research areas of gastroenterology. Since then, the main aim has been to eradicate this microaerophilic bacterium from the stomachs of infected subjects. Methods: We studied symptomatic cases by endoscopic surgery and examined the prevalence of cagA-vacA genotypes among the H. pylori isolates. H. pylori isolated from antral biopsies of patients with gastritis and duodenal ulcer were subjected to antimicrobial susceptibility testing and PCR genotyping by using routine bacterial cultures. Clarithromycin-susceptibility profiling was done by the E-test. DNA was extracted using standard manufacturer protocols with minor modifications and cagA and vacA genotyping was done PCR. Results: In our study, all strains identified as H. pylori in culture (61/81) were confirmed by PCR by amplifying a fragment of the glmM gene. Totally, 61 patients were confirmed to be positive for H. pylori and they were included in the genotyping and antibiotic-susceptibility testing. Thirteen H. pylori strains were determined to be resistant to clarithromycin. Discussion: Current accumulating data indicate that both clarithromycin-resistant and susceptible isolates of H. pylori need to be screened and tracked in populations. SN - 1178-6973 UR - https://www.unboundmedicine.com/medline/citation/31802920/Clarithromycin-Susceptible_But_Virulent_Helicobacter_pylori_Strains_Infecting_Iranian_Patients'_Stomachs L2 - https://dx.doi.org/10.2147/IDR.S223602 DB - PRIME DP - Unbound Medicine ER -