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Significance of TC9 and TH9 in Helicobacter pylori-induced gastritis.
Helicobacter 2020; 25(1):e12672H

Abstract

BACKGROUND

H pylori plays a critical role in the development of stomach cancer, especially in people affected by the bacteria at an early stage of life. Th9 cells and IL-9 play major roles in immune responses against various infections. IL-9 is influential in chronic or acute inflammation of the mucosa.

AIM

This study seeks to investigate the possible functions of Tc9, Th9 cells, and IL-9 level in patients with inflammation due to H pylori infection.

METHODS

Eighty-three patients with dyspepsia symptoms and twenty normal subjects with no sign and symptoms of dyspepsia were recruited. Frequencies of T-cell subsets were determined by flow cytometry. Levels of cytokines IL-9 family in the sera and supernatants of antigen-activated PBMCs patients were measured by ELISA and flow cytometry.

RESULTS

The participants included 56 females and 47 males with a mean age of 39.2 ± 15.3 years. We assigned the infected group into peptic ulcer and gastritis (chronic active and chronic). Frequencies of Tc9, Th17, Tc17, Th17/9, and Tc17/9 increased significantly in the peptic ulcer, chronic active, and chronic gastritis, compared with the uninfected and healthy control groups. A significant increase was seen in IL-9, IL-4, and IL-23 in the chronic active gastritis. Further observed was a significant increase in IL-21 and a decrease in IL-10 in the infected groups.

CONCLUSION

The results revealed that increased Tc9, Th17/9, and Tc17/9 cells appear to be influential in the progression and severity of H pylori infection. Also, increased IL-9 and IL-4 levels and Tc9, Tc17/9, and Th17/9 were seen in chronic active gastritis patients. These findings may provide useful information for a therapeutic targeting of chronic active H pylori infections.

Authors+Show Affiliations

Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Shiraz Institute for Cancer Research, Shiraz University of Medical Science, Shiraz, Iran.Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Department of Health Policy Research Center, Shiraz University of Medical Science, Shiraz, Iran.Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31803999

Citation

Shamsdin, Seyedeh Azra, et al. "Significance of TC9 and TH9 in Helicobacter Pylori-induced Gastritis." Helicobacter, vol. 25, no. 1, 2020, pp. e12672.
Shamsdin SA, Alborzi A, Ghaderi A, et al. Significance of TC9 and TH9 in Helicobacter pylori-induced gastritis. Helicobacter. 2020;25(1):e12672.
Shamsdin, S. A., Alborzi, A., Ghaderi, A., Lankrani, K. B., & Pouladfar, G. R. (2020). Significance of TC9 and TH9 in Helicobacter pylori-induced gastritis. Helicobacter, 25(1), pp. e12672. doi:10.1111/hel.12672.
Shamsdin SA, et al. Significance of TC9 and TH9 in Helicobacter Pylori-induced Gastritis. Helicobacter. 2020;25(1):e12672. PubMed PMID: 31803999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Significance of TC9 and TH9 in Helicobacter pylori-induced gastritis. AU - Shamsdin,Seyedeh Azra, AU - Alborzi,Abdolvahab, AU - Ghaderi,Abbas, AU - Lankrani,Kamran B, AU - Pouladfar,Gholam Reza, Y1 - 2019/12/05/ PY - 2019/04/30/received PY - 2019/10/09/revised PY - 2019/10/16/accepted PY - 2019/12/6/pubmed PY - 2019/12/6/medline PY - 2019/12/6/entrez KW - H pylori KW - Gastritis KW - Infection KW - T cells subsets SP - e12672 EP - e12672 JF - Helicobacter JO - Helicobacter VL - 25 IS - 1 N2 - BACKGROUND: H pylori plays a critical role in the development of stomach cancer, especially in people affected by the bacteria at an early stage of life. Th9 cells and IL-9 play major roles in immune responses against various infections. IL-9 is influential in chronic or acute inflammation of the mucosa. AIM: This study seeks to investigate the possible functions of Tc9, Th9 cells, and IL-9 level in patients with inflammation due to H pylori infection. METHODS: Eighty-three patients with dyspepsia symptoms and twenty normal subjects with no sign and symptoms of dyspepsia were recruited. Frequencies of T-cell subsets were determined by flow cytometry. Levels of cytokines IL-9 family in the sera and supernatants of antigen-activated PBMCs patients were measured by ELISA and flow cytometry. RESULTS: The participants included 56 females and 47 males with a mean age of 39.2 ± 15.3 years. We assigned the infected group into peptic ulcer and gastritis (chronic active and chronic). Frequencies of Tc9, Th17, Tc17, Th17/9, and Tc17/9 increased significantly in the peptic ulcer, chronic active, and chronic gastritis, compared with the uninfected and healthy control groups. A significant increase was seen in IL-9, IL-4, and IL-23 in the chronic active gastritis. Further observed was a significant increase in IL-21 and a decrease in IL-10 in the infected groups. CONCLUSION: The results revealed that increased Tc9, Th17/9, and Tc17/9 cells appear to be influential in the progression and severity of H pylori infection. Also, increased IL-9 and IL-4 levels and Tc9, Tc17/9, and Th17/9 were seen in chronic active gastritis patients. These findings may provide useful information for a therapeutic targeting of chronic active H pylori infections. SN - 1523-5378 UR - https://www.unboundmedicine.com/medline/citation/31803999/Significance_of_TC9_and_TH9_in_Helicobacter_pylori-induced_gastritis L2 - https://doi.org/10.1111/hel.12672 DB - PRIME DP - Unbound Medicine ER -