Tags

Type your tag names separated by a space and hit enter

Protective effects of genipin on ethanol-induced acute gastric injury in mice by inhibiting NLRP3 inflammasome activation.
Eur J Pharmacol 2020; 867:172800EJ

Abstract

Genipin has been shown to exert anti-inflammatory effects, but its mechanism in protecting the ethanol-induced acute gastric injuries remains largely unclear. The present study aimed to investigate the effects of genipin on ethanol-induced acute gastric injuries in mice. After intragastrical administration of genipin for 7 consecutive days, acute gastric injuries were induced in the mice by ethanol treatment for 1 h. The expression levels of MDA, MPO, SOD, CAT, and NO in gastric tissues, and the levels of IL-6, TNF-α, MTL, SP, VIP and SS in serum samples were measured by ELISA. In addition, Western blotting was used to determine the expression levels of proteins involved in NLRP3 signaling pathway. The findings revealed that oral administration of genipin significantly ameliorated the pathological injury of gastric mucosa induced by ethanol, decreased the oxidative stress induced by ethanol and suppressed the expression levels of in-flammatory cytokines in gastric tissues and serum samples. In addition, it was observed that oral administration of genipin could remarkably inhibit the expression levels of related proteins in the NLRP3 signaling pathway. In conclusion, these results suggest that genipin may exhibit protective roles in ethanol-induced gastric mucosal injuries by activating antioxidant system and attenuating inflammatory reaction.

Authors+Show Affiliations

College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, PR China.College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, PR China.College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, PR China.Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan, 38610, Republic of Korea.College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, PR China.College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, PR China.College of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, PR China. Electronic address: wangsm@ccucm.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31805268

Citation

Zhao, Tianyi, et al. "Protective Effects of Genipin On Ethanol-induced Acute Gastric Injury in Mice By Inhibiting NLRP3 Inflammasome Activation." European Journal of Pharmacology, vol. 867, 2020, p. 172800.
Zhao T, Zhang Y, Mu S, et al. Protective effects of genipin on ethanol-induced acute gastric injury in mice by inhibiting NLRP3 inflammasome activation. Eur J Pharmacol. 2020;867:172800.
Zhao, T., Zhang, Y., Mu, S., Park, J. P., Bu, H., Leng, X., & Wang, S. (2020). Protective effects of genipin on ethanol-induced acute gastric injury in mice by inhibiting NLRP3 inflammasome activation. European Journal of Pharmacology, 867, p. 172800. doi:10.1016/j.ejphar.2019.172800.
Zhao T, et al. Protective Effects of Genipin On Ethanol-induced Acute Gastric Injury in Mice By Inhibiting NLRP3 Inflammasome Activation. Eur J Pharmacol. 2020 Jan 15;867:172800. PubMed PMID: 31805268.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of genipin on ethanol-induced acute gastric injury in mice by inhibiting NLRP3 inflammasome activation. AU - Zhao,Tianyi, AU - Zhang,Yue, AU - Mu,Shuangshuang, AU - Park,Jong Pil, AU - Bu,Hongshi, AU - Leng,Xiangyang, AU - Wang,Shumin, Y1 - 2019/12/02/ PY - 2019/10/16/received PY - 2019/11/11/revised PY - 2019/11/14/accepted PY - 2019/12/6/pubmed PY - 2019/12/6/medline PY - 2019/12/6/entrez KW - Gastric ulcer KW - Genipin KW - Inflammasome KW - NLRP3 KW - Oxidative stress SP - 172800 EP - 172800 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 867 N2 - Genipin has been shown to exert anti-inflammatory effects, but its mechanism in protecting the ethanol-induced acute gastric injuries remains largely unclear. The present study aimed to investigate the effects of genipin on ethanol-induced acute gastric injuries in mice. After intragastrical administration of genipin for 7 consecutive days, acute gastric injuries were induced in the mice by ethanol treatment for 1 h. The expression levels of MDA, MPO, SOD, CAT, and NO in gastric tissues, and the levels of IL-6, TNF-α, MTL, SP, VIP and SS in serum samples were measured by ELISA. In addition, Western blotting was used to determine the expression levels of proteins involved in NLRP3 signaling pathway. The findings revealed that oral administration of genipin significantly ameliorated the pathological injury of gastric mucosa induced by ethanol, decreased the oxidative stress induced by ethanol and suppressed the expression levels of in-flammatory cytokines in gastric tissues and serum samples. In addition, it was observed that oral administration of genipin could remarkably inhibit the expression levels of related proteins in the NLRP3 signaling pathway. In conclusion, these results suggest that genipin may exhibit protective roles in ethanol-induced gastric mucosal injuries by activating antioxidant system and attenuating inflammatory reaction. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/31805268/Protective_effects_of_genipin_on_ethanol-induced_acute_gastric_injury_in_mice_by_inhibiting_NLRP3_inflammasome_activation L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(19)30752-6 DB - PRIME DP - Unbound Medicine ER -