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GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis.
Plant Cell Physiol. 2020 Mar 01; 61(3):596-605.PC

Abstract

Auxin is the first discovered plant hormone and is essential for many aspects of plant growth and development. Indole-3-acetic acid (IAA) is the main auxin and plays pivotal roles in intercellular communication through polar auxin transport. Phenylacetic acid (PAA) is another natural auxin that does not show polar movement. Although a wide range of species have been shown to produce PAA, its biosynthesis, inactivation and physiological significance in plants are largely unknown. In this study, we demonstrate that overexpression of the CYP79A2 gene, which is involved in benzylglucosinolate synthesis, remarkably increased the levels of PAA and enhanced lateral root formation in Arabidopsis. This coincided with a significant reduction in the levels of IAA. The results from auxin metabolite quantification suggest that the PAA-dependent induction of GRETCHEN HAGEN 3 (GH3) genes, which encode auxin-amido synthetases, promote the inactivation of IAA. Similarly, an increase in IAA synthesis, via the indole-3-acetaldoxime pathway, significantly reduced the levels of PAA. The same adjustment of IAA and PAA levels was also observed by applying each auxin to wild-type plants. These results show that GH3 auxin-amido synthetases can alter the ratio of IAA and PAA in plant growth and development.

Authors+Show Affiliations

Department of Bioregulation and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, 183-8509 Japan.Laboratory of Biochemistry, Wageningen University & Research, Wageningen 6708 WE, the Netherlands.Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, 183-8509 Japan. JSPS International Research Fellow, The Japan Society for the Promotion of Science (JSPS), Chiyoda-ku, Japan.Department of Biochemistry, Okayama University of Science, Okayama, 700-0005 Japan.Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Fuchu, 183-8509 Japan. RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045 Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31808940

Citation

Aoi, Yuki, et al. "GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis." Plant & Cell Physiology, vol. 61, no. 3, 2020, pp. 596-605.
Aoi Y, Tanaka K, Cook SD, et al. GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis. Plant Cell Physiol. 2020;61(3):596-605.
Aoi, Y., Tanaka, K., Cook, S. D., Hayashi, K. I., & Kasahara, H. (2020). GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis. Plant & Cell Physiology, 61(3), 596-605. https://doi.org/10.1093/pcp/pcz223
Aoi Y, et al. GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis. Plant Cell Physiol. 2020 Mar 1;61(3):596-605. PubMed PMID: 31808940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GH3 Auxin-Amido Synthetases Alter the Ratio of Indole-3-Acetic Acid and Phenylacetic Acid in Arabidopsis. AU - Aoi,Yuki, AU - Tanaka,Keita, AU - Cook,Sam David, AU - Hayashi,Ken-Ichiro, AU - Kasahara,Hiroyuki, PY - 2019/03/12/received PY - 2019/12/02/accepted PY - 2019/12/7/pubmed PY - 2020/11/24/medline PY - 2019/12/7/entrez KW - Arabidopsis KW - Auxin KW - Biosynthesis KW - Inactivation KW - Indole-3-acetic acid KW - Phenylacetic acid SP - 596 EP - 605 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 61 IS - 3 N2 - Auxin is the first discovered plant hormone and is essential for many aspects of plant growth and development. Indole-3-acetic acid (IAA) is the main auxin and plays pivotal roles in intercellular communication through polar auxin transport. Phenylacetic acid (PAA) is another natural auxin that does not show polar movement. Although a wide range of species have been shown to produce PAA, its biosynthesis, inactivation and physiological significance in plants are largely unknown. In this study, we demonstrate that overexpression of the CYP79A2 gene, which is involved in benzylglucosinolate synthesis, remarkably increased the levels of PAA and enhanced lateral root formation in Arabidopsis. This coincided with a significant reduction in the levels of IAA. The results from auxin metabolite quantification suggest that the PAA-dependent induction of GRETCHEN HAGEN 3 (GH3) genes, which encode auxin-amido synthetases, promote the inactivation of IAA. Similarly, an increase in IAA synthesis, via the indole-3-acetaldoxime pathway, significantly reduced the levels of PAA. The same adjustment of IAA and PAA levels was also observed by applying each auxin to wild-type plants. These results show that GH3 auxin-amido synthetases can alter the ratio of IAA and PAA in plant growth and development. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/31808940/GH3_Auxin_Amido_Synthetases_Alter_the_Ratio_of_Indole_3_Acetic_Acid_and_Phenylacetic_Acid_in_Arabidopsis_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcz223 DB - PRIME DP - Unbound Medicine ER -