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Inhibition of miR-214-3p Aids in Preventing Epithelial Ovarian Cancer Malignancy by Increasing the Expression of LHX6.
Cancers (Basel) 2019; 11(12)C

Abstract

In human epithelial ovarian cancer (EOC), various miRNAs can function as either oncogenes or tumor suppressor genes. We investigated miRNAs known to be involved in EOC progression and analyzed their expression in tissues and serum-derived exosomes from benign serous cystadenoma, borderline serous tumor, low-grade serous ovarian cancer, and high-grade serous ovarian cancer patients (HGSO). The HGSO group was divided based on the platinum-free interval, which is defined as the duration from the completion of platinum-based chemotherapy to recurrence. We also analyzed the mRNA levels of target genes that candidate miRNAs might regulate in patient tissues. miR-214-3p was highly expressed in tissues and exosomes derived from EOC with high malignancy and also found to regulate the expression of LIM homeobox domain 6 (LHX6) mRNA. Serum exosomal levels of miR-214-3p were significantly increased in platinum-resistant HGSO (25.2-fold, p < 0.001) compared to the exosomal expression of benign tumor patients. On transfection of miR-214-3p inhibitor in EOC cells, cell proliferation was inhibited while apoptotic cell death was increased. Collectively, we suggest that miR-214-3p in serum exosomes can be a potential biomarker for the diagnosis and prognosis of ovarian tumor, and its inhibition can be a supportive treatment for EOC.

Authors+Show Affiliations

Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul 03080, Korea.Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul 03080, Korea.Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul 03080, Korea.Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul 03080, Korea.Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.Department of Food and Nutrition, Kookmin University, Seoul 02707, Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31810245

Citation

Yang, Changwon, et al. "Inhibition of miR-214-3p Aids in Preventing Epithelial Ovarian Cancer Malignancy By Increasing the Expression of LHX6." Cancers, vol. 11, no. 12, 2019.
Yang C, Kim HS, Park SJ, et al. Inhibition of miR-214-3p Aids in Preventing Epithelial Ovarian Cancer Malignancy by Increasing the Expression of LHX6. Cancers (Basel). 2019;11(12).
Yang, C., Kim, H. S., Park, S. J., Lee, E. J., Kim, S. I., Song, G., & Lim, W. (2019). Inhibition of miR-214-3p Aids in Preventing Epithelial Ovarian Cancer Malignancy by Increasing the Expression of LHX6. Cancers, 11(12), doi:10.3390/cancers11121917.
Yang C, et al. Inhibition of miR-214-3p Aids in Preventing Epithelial Ovarian Cancer Malignancy By Increasing the Expression of LHX6. Cancers (Basel). 2019 Dec 2;11(12) PubMed PMID: 31810245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of miR-214-3p Aids in Preventing Epithelial Ovarian Cancer Malignancy by Increasing the Expression of LHX6. AU - Yang,Changwon, AU - Kim,Hee Seung, AU - Park,Soo Jin, AU - Lee,Eun Ji, AU - Kim,Se Ik, AU - Song,Gwonhwa, AU - Lim,Whasun, Y1 - 2019/12/02/ PY - 2019/11/08/received PY - 2019/11/26/revised PY - 2019/11/26/accepted PY - 2019/12/8/entrez KW - EOC KW - LHX6 KW - apoptosis KW - exosome KW - miR-214-3p JF - Cancers JO - Cancers (Basel) VL - 11 IS - 12 N2 - In human epithelial ovarian cancer (EOC), various miRNAs can function as either oncogenes or tumor suppressor genes. We investigated miRNAs known to be involved in EOC progression and analyzed their expression in tissues and serum-derived exosomes from benign serous cystadenoma, borderline serous tumor, low-grade serous ovarian cancer, and high-grade serous ovarian cancer patients (HGSO). The HGSO group was divided based on the platinum-free interval, which is defined as the duration from the completion of platinum-based chemotherapy to recurrence. We also analyzed the mRNA levels of target genes that candidate miRNAs might regulate in patient tissues. miR-214-3p was highly expressed in tissues and exosomes derived from EOC with high malignancy and also found to regulate the expression of LIM homeobox domain 6 (LHX6) mRNA. Serum exosomal levels of miR-214-3p were significantly increased in platinum-resistant HGSO (25.2-fold, p < 0.001) compared to the exosomal expression of benign tumor patients. On transfection of miR-214-3p inhibitor in EOC cells, cell proliferation was inhibited while apoptotic cell death was increased. Collectively, we suggest that miR-214-3p in serum exosomes can be a potential biomarker for the diagnosis and prognosis of ovarian tumor, and its inhibition can be a supportive treatment for EOC. SN - 2072-6694 UR - https://www.unboundmedicine.com/medline/citation/31810245/Inhibition_of_miR-214-3p_Aids_in_Preventing_Epithelial_Ovarian_Cancer_Malignancy_by_Increasing_the_Expression_of_LHX6 DB - PRIME DP - Unbound Medicine ER -