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Application of oxytocin with low-level laser irradiation suppresses the facilitation of cortical excitability by partial ligation of the infraorbital nerve in rats: An optical imaging study.
Brain Res 2020; 1728:146588BR

Abstract

The effects of current treatments for neuropathic pain are limited. Oxytocin is a novel candidate substance to relieve neuropathic pain, as demonstrated in various animal models with nerve injury. Low-level laser therapy (LLLT) is another option for the treatment of neuropathic pain. In this study, we quantified the effects of oxytocin or LLLT alone and the combination of oxytocin and LLLT on cortical excitation induced by electrical stimulation of the dental pulp using optical imaging with a voltage-sensitive dye in the neuropathic pain model with partial ligation of the infraorbital nerve (pl-ION). We applied oxytocin (OXT, 0.5 μmol) to the rat once on the day of pl-ION locally to the injured nerve. LLLT using a diode laser (810 nm, 0.1 W, 500 s, continuous mode) was performed daily via the skin to the injured nerve from the day of pl-ION to 2 days after pl-ION. Cortical responses to electrical stimulation of the mandibular molar pulp under urethane anesthesia were recorded 3 days after pl-ION. Both the amplitude and area of excitation in the primary and secondary somatosensory and insular cortices in pl-ION rats were larger than those in sham rats. The larger amplitude of cortical excitation caused by pl-ION was suppressed by OXT or LLLT. The expanded area of cortical excitation caused by pl-ION was suppressed by OXT with LLLT but not by OXT or LLLT alone. These results suggest that the combined application of OXT and LLLT is effective in relieving the neuropathic pain induced by trigeminal nerve injury.

Authors+Show Affiliations

Department of Pharmacology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan.Department of Pharmacology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Division of Oral and Craniomaxillofacial Research, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Departament of Biology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan. Electronic address: fujita.satoshi@nihon-u.ac.jp.Department of Pharmacology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan.Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Division of Clinical Research, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan.Department of Orthodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Division of Clinical Research, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan.Department of Pharmacology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Division of Oral and Craniomaxillofacial Research, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101 8310, Japan; Molecular Dynamics Imaging Unit, RIKEN Center for Life Science Technologies, 6-7-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650 0047, Japan. Electronic address: kobayashi.masayuki@nihon-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31811836

Citation

Noma, Daichi, et al. "Application of Oxytocin With Low-level Laser Irradiation Suppresses the Facilitation of Cortical Excitability By Partial Ligation of the Infraorbital Nerve in Rats: an Optical Imaging Study." Brain Research, vol. 1728, 2020, p. 146588.
Noma D, Fujita S, Zama M, et al. Application of oxytocin with low-level laser irradiation suppresses the facilitation of cortical excitability by partial ligation of the infraorbital nerve in rats: An optical imaging study. Brain Res. 2020;1728:146588.
Noma, D., Fujita, S., Zama, M., Mayahara, K., Motoyoshi, M., & Kobayashi, M. (2020). Application of oxytocin with low-level laser irradiation suppresses the facilitation of cortical excitability by partial ligation of the infraorbital nerve in rats: An optical imaging study. Brain Research, 1728, p. 146588. doi:10.1016/j.brainres.2019.146588.
Noma D, et al. Application of Oxytocin With Low-level Laser Irradiation Suppresses the Facilitation of Cortical Excitability By Partial Ligation of the Infraorbital Nerve in Rats: an Optical Imaging Study. Brain Res. 2020 Feb 1;1728:146588. PubMed PMID: 31811836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of oxytocin with low-level laser irradiation suppresses the facilitation of cortical excitability by partial ligation of the infraorbital nerve in rats: An optical imaging study. AU - Noma,Daichi, AU - Fujita,Satoshi, AU - Zama,Manabu, AU - Mayahara,Kotoe, AU - Motoyoshi,Mitsuru, AU - Kobayashi,Masayuki, Y1 - 2019/12/04/ PY - 2019/10/18/received PY - 2019/11/28/revised PY - 2019/12/01/accepted PY - 2019/12/8/pubmed PY - 2019/12/8/medline PY - 2019/12/8/entrez KW - Ectopic pain KW - Insular cortex KW - Nociception KW - Somatosensory cortex KW - Trigeminal nerve KW - Voltage-sensitive dye SP - 146588 EP - 146588 JF - Brain research JO - Brain Res. VL - 1728 N2 - The effects of current treatments for neuropathic pain are limited. Oxytocin is a novel candidate substance to relieve neuropathic pain, as demonstrated in various animal models with nerve injury. Low-level laser therapy (LLLT) is another option for the treatment of neuropathic pain. In this study, we quantified the effects of oxytocin or LLLT alone and the combination of oxytocin and LLLT on cortical excitation induced by electrical stimulation of the dental pulp using optical imaging with a voltage-sensitive dye in the neuropathic pain model with partial ligation of the infraorbital nerve (pl-ION). We applied oxytocin (OXT, 0.5 μmol) to the rat once on the day of pl-ION locally to the injured nerve. LLLT using a diode laser (810 nm, 0.1 W, 500 s, continuous mode) was performed daily via the skin to the injured nerve from the day of pl-ION to 2 days after pl-ION. Cortical responses to electrical stimulation of the mandibular molar pulp under urethane anesthesia were recorded 3 days after pl-ION. Both the amplitude and area of excitation in the primary and secondary somatosensory and insular cortices in pl-ION rats were larger than those in sham rats. The larger amplitude of cortical excitation caused by pl-ION was suppressed by OXT or LLLT. The expanded area of cortical excitation caused by pl-ION was suppressed by OXT with LLLT but not by OXT or LLLT alone. These results suggest that the combined application of OXT and LLLT is effective in relieving the neuropathic pain induced by trigeminal nerve injury. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/31811836/Application_of_oxytocin_with_low-level_laser_irradiation_suppresses_the_facilitation_of_cortical_excitability_by_partial_ligation_of_the_infraorbital_nerve_in_rats:_An_optical_imaging_study L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(19)30642-0 DB - PRIME DP - Unbound Medicine ER -