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Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. 'Moscatel Galego').
BMC Genomics. 2019 Dec 09; 20(1):952.BG

Abstract

BACKGROUND

Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pigmentation, leading to a plethora of different berry skin colors. Color depletion and reversion are often observed in the field. In this study we analyzed the origin of a novel white-to-red skin color reversion and studied its possible metabolic and transcriptomic consequences on cv. 'Muscat à Petits Grains Blancs' (synonym cv. 'Moscatel Galego Branco'), a member of the large family of Muscats.

RESULTS

The mild red-skinned variant (cv. 'Muscat à Petits Grains Rouge', synonym cv. 'Moscatel Galego Roxo'), characterized by a preferential accumulation of di-hydroxylated anthocyanins, showed in heterozygosis a partially-excised Gret1 retrotransposon in the promoter region of the MYBA1 anthocyanin regulator, while MYBA2 was still in homozygosis for its non-functional allele. Through metabolic (anthocyanin, resveratrol and piceid quantifications) and transcriptomic (RNA-Seq) analyses, we show that within a near-isogenic background, the transcriptomic consequences of color reversion are largely associated to diminished light/UV-B responses probably as a consequence of the augment of metabolic sunscreens (i.e. anthocyanins).

CONCLUSIONS

We propose that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification (e.g. methylation and acylation) are the potential causes for the mild red-skinned coloration in the pigmented revertant. The observed positive relation between anthocyanins and stilbenes could be attributable to an increased influx of phenylpropanoid intermediaries due to the replenished activity of MYBA1, an effect yet to be demonstrated in other somatic variants.

Authors+Show Affiliations

Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal. Centre for Plant Biotechnology and Genomics (UPM-INIA, CBGP), Campus de Montegancedo. Autovía M40 km38, 28223 Pozuelo de Alarcón, Madrid, Spain.Institute for Integrative Systems Biology, I2SysBio (Universitat de Valencia - CSIC), 46908, Paterna, Valencia, Spain.Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal.Centre for Plant Biotechnology and Genomics (UPM-INIA, CBGP), Campus de Montegancedo. Autovía M40 km38, 28223 Pozuelo de Alarcón, Madrid, Spain.Centre for Plant Biotechnology and Genomics (UPM-INIA, CBGP), Campus de Montegancedo. Autovía M40 km38, 28223 Pozuelo de Alarcón, Madrid, Spain. rarroyo@inia.es.Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801, Vila Real, Portugal. icastro@utad.pt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31815637

Citation

Ferreira, Vanessa, et al. "Genetic Analysis of a White-to-red Berry Skin Color Reversion and Its Transcriptomic and Metabolic Consequences in Grapevine (Vitis Vinifera Cv. 'Moscatel Galego')." BMC Genomics, vol. 20, no. 1, 2019, p. 952.
Ferreira V, Matus JT, Pinto-Carnide O, et al. Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. 'Moscatel Galego'). BMC Genomics. 2019;20(1):952.
Ferreira, V., Matus, J. T., Pinto-Carnide, O., Carrasco, D., Arroyo-García, R., & Castro, I. (2019). Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. 'Moscatel Galego'). BMC Genomics, 20(1), 952. https://doi.org/10.1186/s12864-019-6237-5
Ferreira V, et al. Genetic Analysis of a White-to-red Berry Skin Color Reversion and Its Transcriptomic and Metabolic Consequences in Grapevine (Vitis Vinifera Cv. 'Moscatel Galego'). BMC Genomics. 2019 Dec 9;20(1):952. PubMed PMID: 31815637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. 'Moscatel Galego'). AU - Ferreira,Vanessa, AU - Matus,José Tomás, AU - Pinto-Carnide,Olinda, AU - Carrasco,David, AU - Arroyo-García,Rosa, AU - Castro,Isaura, Y1 - 2019/12/09/ PY - 2018/08/12/received PY - 2019/10/29/accepted PY - 2019/12/10/entrez PY - 2019/12/10/pubmed PY - 2020/5/6/medline KW - Berry color KW - Grapevine KW - Moscatel Galego KW - RNA-Seq KW - Somatic variation SP - 952 EP - 952 JF - BMC genomics JO - BMC Genomics VL - 20 IS - 1 N2 - BACKGROUND: Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pigmentation, leading to a plethora of different berry skin colors. Color depletion and reversion are often observed in the field. In this study we analyzed the origin of a novel white-to-red skin color reversion and studied its possible metabolic and transcriptomic consequences on cv. 'Muscat à Petits Grains Blancs' (synonym cv. 'Moscatel Galego Branco'), a member of the large family of Muscats. RESULTS: The mild red-skinned variant (cv. 'Muscat à Petits Grains Rouge', synonym cv. 'Moscatel Galego Roxo'), characterized by a preferential accumulation of di-hydroxylated anthocyanins, showed in heterozygosis a partially-excised Gret1 retrotransposon in the promoter region of the MYBA1 anthocyanin regulator, while MYBA2 was still in homozygosis for its non-functional allele. Through metabolic (anthocyanin, resveratrol and piceid quantifications) and transcriptomic (RNA-Seq) analyses, we show that within a near-isogenic background, the transcriptomic consequences of color reversion are largely associated to diminished light/UV-B responses probably as a consequence of the augment of metabolic sunscreens (i.e. anthocyanins). CONCLUSIONS: We propose that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification (e.g. methylation and acylation) are the potential causes for the mild red-skinned coloration in the pigmented revertant. The observed positive relation between anthocyanins and stilbenes could be attributable to an increased influx of phenylpropanoid intermediaries due to the replenished activity of MYBA1, an effect yet to be demonstrated in other somatic variants. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/31815637/Genetic_analysis_of_a_white-to-red_berry_skin_color_reversion_and_its_transcriptomic_and_metabolic_consequences_in_grapevine_(Vitis_vinifera_cv._'Moscatel_Galego') DB - PRIME DP - Unbound Medicine ER -