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Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus from a Riverbank in Japan.
Viruses. 2019 12 04; 11(12)V

Abstract

Giant viruses, like pandoraviruses and mimiviruses, have been discovered from diverse environments, and their broad global distribution has been established. Here, we report two new isolates of Pandoravirus spp. and one Mimivirus sp., named Pandoravirus hades, Pandoravirus persephone, and Mimivirus sp. isolate styx, co-isolated from riverbank soil in Japan. We obtained nearly complete sequences of the family B DNA polymerase gene (polB) of P. hades and P. persephone; the former carried two known intein regions, while the latter had only one. Phylogenetic analysis revealed that the two new pandoravirus isolates are closely related to Pandoravirus dulcis. Furthermore, random amplified polymorphic DNA analysis revealed that P. hades and P. persephone might harbor different genome structures. Based on phylogenetic analysis of the partial polB sequence, Mimivirus sp. isolate styx belongs to mimivirus lineage A. DNA staining suggested that the Pandoravirus spp. asynchronously replicates in amoeba cells while Mimivirus sp. replicates synchronously. We also observed that P. persephone- or Mimivirus sp. isolate styx-infected amoeba cytoplasm is extruded by the cells. To the best of our knowledge, we are the first to report the isolation of pandoraviruses in Asia. In addition, our results emphasize the importance of virus isolation from soil to reveal the ecology of giant viruses.

Authors+Show Affiliations

Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Shinjuku, Tokyo 162-8601, Japan.Laboratory of Biology, Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Shinjuku, Tokyo 162-8601, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31817274

Citation

Akashi, Motohiro, and Masaharu Takemura. "Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus From a Riverbank in Japan." Viruses, vol. 11, no. 12, 2019.
Akashi M, Takemura M. Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus from a Riverbank in Japan. Viruses. 2019;11(12).
Akashi, M., & Takemura, M. (2019). Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus from a Riverbank in Japan. Viruses, 11(12). https://doi.org/10.3390/v11121123
Akashi M, Takemura M. Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus From a Riverbank in Japan. Viruses. 2019 12 4;11(12) PubMed PMID: 31817274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-Isolation and Characterization of Two Pandoraviruses and a Mimivirus from a Riverbank in Japan. AU - Akashi,Motohiro, AU - Takemura,Masaharu, Y1 - 2019/12/04/ PY - 2019/09/25/received PY - 2019/11/14/revised PY - 2019/11/19/accepted PY - 2019/12/11/entrez PY - 2019/12/11/pubmed PY - 2020/9/22/medline KW - DNA polymerase KW - giant virus KW - intein KW - mimivirus KW - pandoravirus KW - replication cycle KW - virus diversity JF - Viruses JO - Viruses VL - 11 IS - 12 N2 - Giant viruses, like pandoraviruses and mimiviruses, have been discovered from diverse environments, and their broad global distribution has been established. Here, we report two new isolates of Pandoravirus spp. and one Mimivirus sp., named Pandoravirus hades, Pandoravirus persephone, and Mimivirus sp. isolate styx, co-isolated from riverbank soil in Japan. We obtained nearly complete sequences of the family B DNA polymerase gene (polB) of P. hades and P. persephone; the former carried two known intein regions, while the latter had only one. Phylogenetic analysis revealed that the two new pandoravirus isolates are closely related to Pandoravirus dulcis. Furthermore, random amplified polymorphic DNA analysis revealed that P. hades and P. persephone might harbor different genome structures. Based on phylogenetic analysis of the partial polB sequence, Mimivirus sp. isolate styx belongs to mimivirus lineage A. DNA staining suggested that the Pandoravirus spp. asynchronously replicates in amoeba cells while Mimivirus sp. replicates synchronously. We also observed that P. persephone- or Mimivirus sp. isolate styx-infected amoeba cytoplasm is extruded by the cells. To the best of our knowledge, we are the first to report the isolation of pandoraviruses in Asia. In addition, our results emphasize the importance of virus isolation from soil to reveal the ecology of giant viruses. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/31817274/Co_Isolation_and_Characterization_of_Two_Pandoraviruses_and_a_Mimivirus_from_a_Riverbank_in_Japan_ L2 - https://www.mdpi.com/resolver?pii=v11121123 DB - PRIME DP - Unbound Medicine ER -