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Characterization of a wheat-tetraploid Thinopyrum elongatum 1E(1D) substitution line K17-841-1 by cytological and phenotypic analysis and developed molecular markers.
BMC Genomics. 2019 Dec 10; 20(1):963.BG

Abstract

BACKGROUND

Tetraploid Thinopyrum elongatum (2n = 4x = 28) is a promising source of useful genes, including those related to adaptability and resistance to diverse biotic (Fusarium head blight, rust, powdery mildew, and yellow dwarf virus) and abiotic (cold, drought, and salt) stresses. However, gene transfer rates are low for this species and relatively few species-specific molecular markers are available.

RESULTS

The wheat-tetraploid Th. elongatum line K17-841-1 derived from a cross between a hexaploid Trititrigia and Sichuan wheat cultivars was characterized based on sequential genomic and fluorescence in situ hybridizations and simple sequence repeat markers. We revealed that K17-841-1 is a 1E (1D) chromosomal substitution line that is highly resistant to stripe rust pathogen strains prevalent in China. By comparing the sequences generated during genotyping-by-sequencing (GBS), we obtained 597 specific fragments on the 1E chromosome of tetraploid Th. elongatum. A total of 235 primers were designed and 165 new Th. elongatum-specific markers were developed, with an efficiency of up to 70%. Marker validation analyses indicated that 25 specific markers can discriminate between the tetraploid Th. elongatum chromosomes and the chromosomes of other wheat-related species. An evaluation of the utility of these markers in a F2 breeding population suggested these markers are linked to the stripe rust resistance gene on chromosome 1E. Furthermore, 28 markers are unique to diploid Th. elongatum, tetraploid Th. elongatum, or decaploid Thinopyrum ponticum, which carry the E genome. Finally, 48 and 74 markers revealed polymorphisms between Thinopyrum E-genome- containing species and Thinopyrum bessarabicum (Eb) and Pseudoroegneria libanotica (St), respectively.

CONCLUSIONS

This new substitution line provide appropriate bridge-breeding-materials for alien gene introgression to improve wheat stripe rust resistance. The markers developed using GBS technology in this study may be useful for the high-throughput and accurate detection of tetraploid Th. elongatum DNA in diverse materials. They may also be relevant for investigating the genetic differences and phylogenetic relationships among E, Eb, St, and other closely-related genomes and for further characterizing these complex species.

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Authors+Show Affiliations

Li D
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhang J
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Liu H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Tan B
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhu W
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xu L
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Wang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zeng J
College of Resources, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Fan X
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Sha L
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Ma J
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Chen G
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Zhou Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Kang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. houyang.kang@sicau.edu.cn. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. houyang.kang@sicau.edu.cn.

MeSH

Chromosome MappingChromosomes, PlantDisease ResistanceGenetic MarkersGenome, PlantGenotypeIn Situ Hybridization, FluorescenceMicrosatellite RepeatsPhenotypePlant DiseasesPoaceaePolymorphism, GeneticTetraploidyTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31823771

Citation

Li, Daiyan, et al. "Characterization of a Wheat-tetraploid Thinopyrum Elongatum 1E(1D) Substitution Line K17-841-1 By Cytological and Phenotypic Analysis and Developed Molecular Markers." BMC Genomics, vol. 20, no. 1, 2019, p. 963.
Li D, Zhang J, Liu H, et al. Characterization of a wheat-tetraploid Thinopyrum elongatum 1E(1D) substitution line K17-841-1 by cytological and phenotypic analysis and developed molecular markers. BMC Genomics. 2019;20(1):963.
Li, D., Zhang, J., Liu, H., Tan, B., Zhu, W., Xu, L., Wang, Y., Zeng, J., Fan, X., Sha, L., Zhang, H., Ma, J., Chen, G., Zhou, Y., & Kang, H. (2019). Characterization of a wheat-tetraploid Thinopyrum elongatum 1E(1D) substitution line K17-841-1 by cytological and phenotypic analysis and developed molecular markers. BMC Genomics, 20(1), 963. https://doi.org/10.1186/s12864-019-6359-9
Li D, et al. Characterization of a Wheat-tetraploid Thinopyrum Elongatum 1E(1D) Substitution Line K17-841-1 By Cytological and Phenotypic Analysis and Developed Molecular Markers. BMC Genomics. 2019 Dec 10;20(1):963. PubMed PMID: 31823771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a wheat-tetraploid Thinopyrum elongatum 1E(1D) substitution line K17-841-1 by cytological and phenotypic analysis and developed molecular markers. AU - Li,Daiyan, AU - Zhang,Juwei, AU - Liu,Haijiao, AU - Tan,Binwen, AU - Zhu,Wei, AU - Xu,Lili, AU - Wang,Yi, AU - Zeng,Jian, AU - Fan,Xing, AU - Sha,Lina, AU - Zhang,Haiqin, AU - Ma,Jian, AU - Chen,Guoyue, AU - Zhou,Yonghong, AU - Kang,Houyang, Y1 - 2019/12/10/ PY - 2019/07/19/received PY - 2019/12/01/accepted PY - 2019/12/12/entrez PY - 2019/12/12/pubmed PY - 2020/5/6/medline KW - Chromosome substitution line KW - GBS KW - Species- specific molecular markers KW - Stripe rust KW - Tetraploid Thinopyrum elongatum SP - 963 EP - 963 JF - BMC genomics JO - BMC Genomics VL - 20 IS - 1 N2 - BACKGROUND: Tetraploid Thinopyrum elongatum (2n = 4x = 28) is a promising source of useful genes, including those related to adaptability and resistance to diverse biotic (Fusarium head blight, rust, powdery mildew, and yellow dwarf virus) and abiotic (cold, drought, and salt) stresses. However, gene transfer rates are low for this species and relatively few species-specific molecular markers are available. RESULTS: The wheat-tetraploid Th. elongatum line K17-841-1 derived from a cross between a hexaploid Trititrigia and Sichuan wheat cultivars was characterized based on sequential genomic and fluorescence in situ hybridizations and simple sequence repeat markers. We revealed that K17-841-1 is a 1E (1D) chromosomal substitution line that is highly resistant to stripe rust pathogen strains prevalent in China. By comparing the sequences generated during genotyping-by-sequencing (GBS), we obtained 597 specific fragments on the 1E chromosome of tetraploid Th. elongatum. A total of 235 primers were designed and 165 new Th. elongatum-specific markers were developed, with an efficiency of up to 70%. Marker validation analyses indicated that 25 specific markers can discriminate between the tetraploid Th. elongatum chromosomes and the chromosomes of other wheat-related species. An evaluation of the utility of these markers in a F2 breeding population suggested these markers are linked to the stripe rust resistance gene on chromosome 1E. Furthermore, 28 markers are unique to diploid Th. elongatum, tetraploid Th. elongatum, or decaploid Thinopyrum ponticum, which carry the E genome. Finally, 48 and 74 markers revealed polymorphisms between Thinopyrum E-genome- containing species and Thinopyrum bessarabicum (Eb) and Pseudoroegneria libanotica (St), respectively. CONCLUSIONS: This new substitution line provide appropriate bridge-breeding-materials for alien gene introgression to improve wheat stripe rust resistance. The markers developed using GBS technology in this study may be useful for the high-throughput and accurate detection of tetraploid Th. elongatum DNA in diverse materials. They may also be relevant for investigating the genetic differences and phylogenetic relationships among E, Eb, St, and other closely-related genomes and for further characterizing these complex species. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/31823771/Characterization_of_a_wheat_tetraploid_Thinopyrum_elongatum_1E_1D__substitution_line_K17_841_1_by_cytological_and_phenotypic_analysis_and_developed_molecular_markers_ DB - PRIME DP - Unbound Medicine ER -