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Impact of human-derived hemoglobin based oxygen vesicles as a machine perfusion solution for liver donation after cardiac death in a pig model.
PLoS One. 2019; 14(12):e0226183.Plos

Abstract

The recent clinical application of perfusion technology for the machine preservation of donation after cardiac death (DCD) grafts has some advantages. Oxygenation has been proposed for the preservation of DCD liver grafts. The aim of this study is to clarify whether the use of HbV-containing preservation solution during the subnormothermic machine perfusion (SNMP) of the liver graft improves the graft function of DCD porcine livers in an ex vivo reperfusion model. Pig livers were excised after 60 minutes of warm ischemic time and were preserved under one of three preservation conditions for 4 hours. The preservation conditions were as follows: 4°C cold storage (CS group; N = 5), Hypothermic machine preservation (HMP) with UW gluconate solution (HMP group; N = 5), SNMP (21°C) with UW gluconate solution (SNMP group; N = 5), SNMP (21°C) with HbVs (Hb; 1.8 mg/dl) perfusate (SNMP+HbV group; N = 5). Autologous blood perfusion was performed for 2 hours in an isolated liver reperfusion model (IRM). The oxygen consumption of the SNMP and SNMP+HbV group was higher than the HMP groups (p < 0.05). During the reperfusion, the AST level in the SNMP+HbV group was lower than that in the CS, HMP and SNMP groups. The changes in pH after reperfusion was significantly lower in SNMP+HbV group than CS and HMP groups. The ultrastructural findings indicated that the mitochondria of the SNMP+HbV group was well maintained in comparison to the CS, HMP and SNMP groups. The SNMP+HbVs preservation solution protected against metabolic acidosis and preserved the liver function after reperfusion injury in the DCD liver.

Authors+Show Affiliations

Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Mechanical Engineering, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.Department of Mechanical Engineering, Tokyo Metropolitan University, Hachioji-shi, Tokyo, Japan.Department of Pathology, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Cell Physiology, The Jikei University School of Medicine, Minato-ku, Tokyo, Japan.Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Pediatrics, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.Department of Chemistry, Nara Medical University, Kashihara-shi, Nara, Japan.Department of Surgery, Asahikawa Medical University, Asahikawa-shi, Hokkaido, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31825976

Citation

Shonaka, Tatsuya, et al. "Impact of Human-derived Hemoglobin Based Oxygen Vesicles as a Machine Perfusion Solution for Liver Donation After Cardiac Death in a Pig Model." PloS One, vol. 14, no. 12, 2019, pp. e0226183.
Shonaka T, Matsuno N, Obara H, et al. Impact of human-derived hemoglobin based oxygen vesicles as a machine perfusion solution for liver donation after cardiac death in a pig model. PLoS ONE. 2019;14(12):e0226183.
Shonaka, T., Matsuno, N., Obara, H., Yoshikawa, R., Nishikawa, Y., Ishihara, Y., Bochimoto, H., Gochi, M., Otani, M., Kanazawa, H., Azuma, H., Sakai, H., & Furukawa, H. (2019). Impact of human-derived hemoglobin based oxygen vesicles as a machine perfusion solution for liver donation after cardiac death in a pig model. PloS One, 14(12), e0226183. https://doi.org/10.1371/journal.pone.0226183
Shonaka T, et al. Impact of Human-derived Hemoglobin Based Oxygen Vesicles as a Machine Perfusion Solution for Liver Donation After Cardiac Death in a Pig Model. PLoS ONE. 2019;14(12):e0226183. PubMed PMID: 31825976.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of human-derived hemoglobin based oxygen vesicles as a machine perfusion solution for liver donation after cardiac death in a pig model. AU - Shonaka,Tatsuya, AU - Matsuno,Naoto, AU - Obara,Hiromichi, AU - Yoshikawa,Ryo, AU - Nishikawa,Yuji, AU - Ishihara,Yo, AU - Bochimoto,Hiroki, AU - Gochi,Mikako, AU - Otani,Masahide, AU - Kanazawa,Hiroyuki, AU - Azuma,Hiroshi, AU - Sakai,Hiromi, AU - Furukawa,Hiroyuki, Y1 - 2019/12/11/ PY - 2019/03/07/received PY - 2019/11/21/accepted PY - 2019/12/12/entrez PY - 2019/12/12/pubmed PY - 2020/4/1/medline SP - e0226183 EP - e0226183 JF - PloS one JO - PLoS ONE VL - 14 IS - 12 N2 - The recent clinical application of perfusion technology for the machine preservation of donation after cardiac death (DCD) grafts has some advantages. Oxygenation has been proposed for the preservation of DCD liver grafts. The aim of this study is to clarify whether the use of HbV-containing preservation solution during the subnormothermic machine perfusion (SNMP) of the liver graft improves the graft function of DCD porcine livers in an ex vivo reperfusion model. Pig livers were excised after 60 minutes of warm ischemic time and were preserved under one of three preservation conditions for 4 hours. The preservation conditions were as follows: 4°C cold storage (CS group; N = 5), Hypothermic machine preservation (HMP) with UW gluconate solution (HMP group; N = 5), SNMP (21°C) with UW gluconate solution (SNMP group; N = 5), SNMP (21°C) with HbVs (Hb; 1.8 mg/dl) perfusate (SNMP+HbV group; N = 5). Autologous blood perfusion was performed for 2 hours in an isolated liver reperfusion model (IRM). The oxygen consumption of the SNMP and SNMP+HbV group was higher than the HMP groups (p < 0.05). During the reperfusion, the AST level in the SNMP+HbV group was lower than that in the CS, HMP and SNMP groups. The changes in pH after reperfusion was significantly lower in SNMP+HbV group than CS and HMP groups. The ultrastructural findings indicated that the mitochondria of the SNMP+HbV group was well maintained in comparison to the CS, HMP and SNMP groups. The SNMP+HbVs preservation solution protected against metabolic acidosis and preserved the liver function after reperfusion injury in the DCD liver. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31825976/Impact_of_human_derived_hemoglobin_based_oxygen_vesicles_as_a_machine_perfusion_solution_for_liver_donation_after_cardiac_death_in_a_pig_model_ L2 - http://dx.plos.org/10.1371/journal.pone.0226183 DB - PRIME DP - Unbound Medicine ER -