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Overexpressing GH3.1 and GH3.1L reduces susceptibility to Xanthomonas citri subsp. citri by repressing auxin signaling in citrus (Citrus sinensis Osbeck).
PLoS One. 2019; 14(12):e0220017.Plos

Abstract

The auxin early response gene Gretchen Hagen3 (GH3) plays dual roles in plant development and responses to biotic or abiotic stress. It functions in regulating hormone homeostasis through the conjugation of free auxin to amino acids. In citrus, GH3.1 and GH3.1L play important roles in responding to Xanthomonas citri subsp. citri (Xcc). Here, in Wanjingcheng orange (Citrus sinensis Osbeck), the overexpression of CsGH3.1 and CsGH3.1L caused increased branching and drooping dwarfism, as well as smaller, thinner and upward curling leaves compared with wild-type. Hormone determinations showed that overexpressing CsGH3.1 and CsGH3.1L decreased the free auxin contents and accelerated the Xcc-induced decline of free auxin levels in transgenic plants. A resistance analysis showed that transgenic plants had reduced susceptibility to citrus canker, and a transcriptomic analysis revealed that hormone signal transduction-related pathways were significantly affected by the overexpression of CsGH3.1 and CsGH3.1L. A MapMan analysis further showed that overexpressing either of these two genes significantly downregulated the expression levels of the annotated auxin/indole-3-acetic acid family genes and significantly upregulated biotic stress-related functions and pathways. Salicylic acid, jasmonic acid, abscisic acid, ethylene and zeatin levels in transgenic plants displayed obvious changes compared with wild-type. In particular, the salicylic acid and ethylene levels involved in plant resistance responses markedly increased in transgenic plants. Thus, the overexpression of CsGH3.1 and CsGH3.1L reduces plant susceptibility to citrus canker by repressing auxin signaling and enhancing defense responses. Our study demonstrates auxin homeostasis' potential in engineering disease resistance in citrus.

Authors+Show Affiliations

Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.Citrus Research Institute, Chinese Academy of Agricultural Sciences and National Center for Citrus Variety Improvement, Chongqing, P. R. China. Citrus Research Institute, Southwest University, Chongqing, P. R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

31830052

Citation

Zou, Xiuping, et al. "Overexpressing GH3.1 and GH3.1L Reduces Susceptibility to Xanthomonas Citri Subsp. Citri By Repressing Auxin Signaling in Citrus (Citrus Sinensis Osbeck)." PloS One, vol. 14, no. 12, 2019, pp. e0220017.
Zou X, Long J, Zhao K, et al. Overexpressing GH3.1 and GH3.1L reduces susceptibility to Xanthomonas citri subsp. citri by repressing auxin signaling in citrus (Citrus sinensis Osbeck). PLoS ONE. 2019;14(12):e0220017.
Zou, X., Long, J., Zhao, K., Peng, A., Chen, M., Long, Q., He, Y., & Chen, S. (2019). Overexpressing GH3.1 and GH3.1L reduces susceptibility to Xanthomonas citri subsp. citri by repressing auxin signaling in citrus (Citrus sinensis Osbeck). PloS One, 14(12), e0220017. https://doi.org/10.1371/journal.pone.0220017
Zou X, et al. Overexpressing GH3.1 and GH3.1L Reduces Susceptibility to Xanthomonas Citri Subsp. Citri By Repressing Auxin Signaling in Citrus (Citrus Sinensis Osbeck). PLoS ONE. 2019;14(12):e0220017. PubMed PMID: 31830052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpressing GH3.1 and GH3.1L reduces susceptibility to Xanthomonas citri subsp. citri by repressing auxin signaling in citrus (Citrus sinensis Osbeck). AU - Zou,Xiuping, AU - Long,Junhong, AU - Zhao,Ke, AU - Peng,Aihong, AU - Chen,Min, AU - Long,Qin, AU - He,Yongrui, AU - Chen,Shanchun, Y1 - 2019/12/12/ PY - 2019/06/27/received PY - 2019/11/16/accepted PY - 2019/12/13/entrez PY - 2019/12/13/pubmed PY - 2020/3/26/medline SP - e0220017 EP - e0220017 JF - PloS one JO - PLoS ONE VL - 14 IS - 12 N2 - The auxin early response gene Gretchen Hagen3 (GH3) plays dual roles in plant development and responses to biotic or abiotic stress. It functions in regulating hormone homeostasis through the conjugation of free auxin to amino acids. In citrus, GH3.1 and GH3.1L play important roles in responding to Xanthomonas citri subsp. citri (Xcc). Here, in Wanjingcheng orange (Citrus sinensis Osbeck), the overexpression of CsGH3.1 and CsGH3.1L caused increased branching and drooping dwarfism, as well as smaller, thinner and upward curling leaves compared with wild-type. Hormone determinations showed that overexpressing CsGH3.1 and CsGH3.1L decreased the free auxin contents and accelerated the Xcc-induced decline of free auxin levels in transgenic plants. A resistance analysis showed that transgenic plants had reduced susceptibility to citrus canker, and a transcriptomic analysis revealed that hormone signal transduction-related pathways were significantly affected by the overexpression of CsGH3.1 and CsGH3.1L. A MapMan analysis further showed that overexpressing either of these two genes significantly downregulated the expression levels of the annotated auxin/indole-3-acetic acid family genes and significantly upregulated biotic stress-related functions and pathways. Salicylic acid, jasmonic acid, abscisic acid, ethylene and zeatin levels in transgenic plants displayed obvious changes compared with wild-type. In particular, the salicylic acid and ethylene levels involved in plant resistance responses markedly increased in transgenic plants. Thus, the overexpression of CsGH3.1 and CsGH3.1L reduces plant susceptibility to citrus canker by repressing auxin signaling and enhancing defense responses. Our study demonstrates auxin homeostasis' potential in engineering disease resistance in citrus. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/31830052/Overexpressing_GH3_1_and_GH3_1L_reduces_susceptibility_to_Xanthomonas_citri_subsp__citri_by_repressing_auxin_signaling_in_citrus__Citrus_sinensis_Osbeck__ L2 - http://dx.plos.org/10.1371/journal.pone.0220017 DB - PRIME DP - Unbound Medicine ER -