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The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes.
Int Microbiol. 2020 Aug; 23(3):381-390.IM

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important gram-negative intracellular pathogen that infects humans and animals. More than 50 putative regulatory proteins have been identified in the S. Typhimurium genome, but few have been clearly defined. In this study, the physiological function and regulatory role of STM14_3563, which encodes a ParD family putative transcriptional regulator in S. Typhimurium, were investigated. Macrophage replication assays and mice experiments revealed that S. Typhimurium showed reduced growth in murine macrophages and attenuated virulence in mice owing to deletion of STM14_3563 gene. RNA sequencing (RNA-Seq) data showed that STM14_3563 exerts wide-ranging effects on gene expression in S. Typhimurium. STM14_3563 activates the expression of several genes encoded in Salmonella pathogenicity island (SPI)-6, SPI-12, and SPI-13, which are required for intracellular replication of S. Typhimurium. Additionally, the global transcriptional regulator Fis was found to directly activate STM14_3563 expression by binding to the STM14_3563 promoter. These results indicate that STM14_3563 is involved in the regulation of a variety of virulence-related genes in S. Typhimurium that contribute to its growth in macrophages and virulence in mice.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Song X
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China. College of Life Sciences, Nankai University, Tianjin, 300071, China.
Zhang H
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China. College of Life Sciences, Nankai University, Tianjin, 300071, China.
Liu X
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China.
Yuan J
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China.
Wang P
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China. College of Life Sciences, Nankai University, Tianjin, 300071, China.
Lv R
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China.
Yang B
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China.
Huang D
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China.
Jiang L
TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300457, China. jianglingyan@nankai.edu.cn. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, 300071, China. jianglingyan@nankai.edu.cn. Tianjin Key Laboratory of Microbial Functional Genomics, Tianjin, 300457, China. jianglingyan@nankai.edu.cn.

MeSH

AnimalsBacterial ProteinsDNA-Binding ProteinsGene Expression Regulation, BacterialGenomic IslandsMacrophagesMiceSalmonella InfectionsSalmonella typhimuriumTranscription FactorsTranscriptomeVirulenceVirulence Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31832871

Citation

Song, Xiaorui, et al. "The Putative Transcriptional Regulator STM14_3563 Facilitates Salmonella Typhimurium Pathogenicity By Activating Virulence-related Genes." International Microbiology : the Official Journal of the Spanish Society for Microbiology, vol. 23, no. 3, 2020, pp. 381-390.
Song X, Zhang H, Liu X, et al. The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes. Int Microbiol. 2020;23(3):381-390.
Song, X., Zhang, H., Liu, X., Yuan, J., Wang, P., Lv, R., Yang, B., Huang, D., & Jiang, L. (2020). The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes. International Microbiology : the Official Journal of the Spanish Society for Microbiology, 23(3), 381-390. https://doi.org/10.1007/s10123-019-00110-3
Song X, et al. The Putative Transcriptional Regulator STM14_3563 Facilitates Salmonella Typhimurium Pathogenicity By Activating Virulence-related Genes. Int Microbiol. 2020;23(3):381-390. PubMed PMID: 31832871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The putative transcriptional regulator STM14_3563 facilitates Salmonella Typhimurium pathogenicity by activating virulence-related genes. AU - Song,Xiaorui, AU - Zhang,Huan, AU - Liu,Xiaoqian, AU - Yuan,Jian, AU - Wang,Peisheng, AU - Lv,Runxia, AU - Yang,Bin, AU - Huang,Di, AU - Jiang,Lingyan, Y1 - 2019/12/13/ PY - 2019/08/06/received PY - 2019/11/28/accepted PY - 2019/11/24/revised PY - 2019/12/14/pubmed PY - 2020/12/15/medline PY - 2019/12/14/entrez KW - Fis KW - Growth in macrophage KW - Regulatory protein KW - STM14_3563 KW - Salmonella Typhimurium KW - Virulence SP - 381 EP - 390 JF - International microbiology : the official journal of the Spanish Society for Microbiology JO - Int Microbiol VL - 23 IS - 3 N2 - Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important gram-negative intracellular pathogen that infects humans and animals. More than 50 putative regulatory proteins have been identified in the S. Typhimurium genome, but few have been clearly defined. In this study, the physiological function and regulatory role of STM14_3563, which encodes a ParD family putative transcriptional regulator in S. Typhimurium, were investigated. Macrophage replication assays and mice experiments revealed that S. Typhimurium showed reduced growth in murine macrophages and attenuated virulence in mice owing to deletion of STM14_3563 gene. RNA sequencing (RNA-Seq) data showed that STM14_3563 exerts wide-ranging effects on gene expression in S. Typhimurium. STM14_3563 activates the expression of several genes encoded in Salmonella pathogenicity island (SPI)-6, SPI-12, and SPI-13, which are required for intracellular replication of S. Typhimurium. Additionally, the global transcriptional regulator Fis was found to directly activate STM14_3563 expression by binding to the STM14_3563 promoter. These results indicate that STM14_3563 is involved in the regulation of a variety of virulence-related genes in S. Typhimurium that contribute to its growth in macrophages and virulence in mice. SN - 1618-1905 UR - https://www.unboundmedicine.com/medline/citation/31832871/The_putative_transcriptional_regulator_STM14_3563_facilitates_Salmonella_Typhimurium_pathogenicity_by_activating_virulence_related_genes_ DB - PRIME DP - Unbound Medicine ER -