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Fluoroquinolone resistance conferred by gyrA, parC mutations, and AbaQ efflux pump among Acinetobacter baumannii clinical isolates causing ventilator-associated pneumonia.
Acta Microbiol Immunol Hung. 2019 Dec 13; 67(4):234-238.AM

Abstract

Acinetobacter baumannii has emerged as an important nosocomial pathogen due to its ability to survive in hospital settings and its antimicrobial resistance. It is one of the key pathogens in ventilator-associated pneumonia (VAP). The aim of this study was to characterize the mechanisms of quinolone resistance among A. baumannii isolates causing VAP and to investigate the presence of the novel abaQ gene among them. Quinolone-resistant A. baumannii isolates causing VAP were collected over a period of 4 months. Mutations within gyrA and parC were analyzed and the presence of qnrA, qnrB, qnrS, and abaQ was investigated genotypically. Twenty-one A. baumannii isolates were collected, most of them (76.2%) were extensively drug-resistant (XDR) and only one isolate (4.8%) was pandrug-resistant (PDR). All isolates showed high level of resistance to ciprofloxacin, while qnrA, qnrB and qnrS were absent among our isolates. This is the first report of A. baumannii isolates co-harboring Ser81Leu in gyrA and Ser84Leu in parC together with the novel abaQ gene. Interestingly, a new mutation in gyrA quinolone resistance-determining region Arg89Cys was detected among two of our isolates. The emergence of XDR and PDR isolates among A. baumannii causing VAP is an alarming threat.

Authors+Show Affiliations

1Microbiology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.2Department of Microbiology and Immunology, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria, Alexandria, Egypt. 3Alexandria University Hospital, Alexandria University, Alexandria, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31833383

Citation

Attia, Nancy M., and Amira Elbaradei. "Fluoroquinolone Resistance Conferred By gyrA, parC Mutations, and AbaQ Efflux Pump Among Acinetobacter Baumannii Clinical Isolates Causing Ventilator-associated Pneumonia." Acta Microbiologica Et Immunologica Hungarica, vol. 67, no. 4, 2019, pp. 234-238.
Attia NM, Elbaradei A. Fluoroquinolone resistance conferred by gyrA, parC mutations, and AbaQ efflux pump among Acinetobacter baumannii clinical isolates causing ventilator-associated pneumonia. Acta Microbiol Immunol Hung. 2019;67(4):234-238.
Attia, N. M., & Elbaradei, A. (2019). Fluoroquinolone resistance conferred by gyrA, parC mutations, and AbaQ efflux pump among Acinetobacter baumannii clinical isolates causing ventilator-associated pneumonia. Acta Microbiologica Et Immunologica Hungarica, 67(4), 234-238. https://doi.org/10.1556/030.66.2019.040
Attia NM, Elbaradei A. Fluoroquinolone Resistance Conferred By gyrA, parC Mutations, and AbaQ Efflux Pump Among Acinetobacter Baumannii Clinical Isolates Causing Ventilator-associated Pneumonia. Acta Microbiol Immunol Hung. 2019 Dec 13;67(4):234-238. PubMed PMID: 31833383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluoroquinolone resistance conferred by gyrA, parC mutations, and AbaQ efflux pump among Acinetobacter baumannii clinical isolates causing ventilator-associated pneumonia. AU - Attia,Nancy M, AU - Elbaradei,Amira, Y1 - 2019/12/13/ PY - 2019/09/21/received PY - 2019/10/14/accepted PY - 2019/12/14/pubmed PY - 2021/7/16/medline PY - 2019/12/14/entrez KW - Acinetobacter baumannii KW - MALDI-TOF MS KW - abaQ KW - gyrA KW - parC KW - quinolone resistance SP - 234 EP - 238 JF - Acta microbiologica et immunologica Hungarica JO - Acta Microbiol Immunol Hung VL - 67 IS - 4 N2 - Acinetobacter baumannii has emerged as an important nosocomial pathogen due to its ability to survive in hospital settings and its antimicrobial resistance. It is one of the key pathogens in ventilator-associated pneumonia (VAP). The aim of this study was to characterize the mechanisms of quinolone resistance among A. baumannii isolates causing VAP and to investigate the presence of the novel abaQ gene among them. Quinolone-resistant A. baumannii isolates causing VAP were collected over a period of 4 months. Mutations within gyrA and parC were analyzed and the presence of qnrA, qnrB, qnrS, and abaQ was investigated genotypically. Twenty-one A. baumannii isolates were collected, most of them (76.2%) were extensively drug-resistant (XDR) and only one isolate (4.8%) was pandrug-resistant (PDR). All isolates showed high level of resistance to ciprofloxacin, while qnrA, qnrB and qnrS were absent among our isolates. This is the first report of A. baumannii isolates co-harboring Ser81Leu in gyrA and Ser84Leu in parC together with the novel abaQ gene. Interestingly, a new mutation in gyrA quinolone resistance-determining region Arg89Cys was detected among two of our isolates. The emergence of XDR and PDR isolates among A. baumannii causing VAP is an alarming threat. SN - 1588-2640 UR - https://www.unboundmedicine.com/medline/citation/31833383/Fluoroquinolone_resistance_conferred_by_gyrA_parC_mutations_and_AbaQ_efflux_pump_among_Acinetobacter_baumannii_clinical_isolates_causing_ventilator_associated_pneumonia_ L2 - https://akjournals.com/doi/10.1556/030.66.2019.040 DB - PRIME DP - Unbound Medicine ER -