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Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli.
Mar Drugs. 2019 Dec 10; 17(12)MD

Abstract

Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88. A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.

Authors+Show Affiliations

College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Xiamen Key Laboratory of Marine Functional Food, Xiamen 361021, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31835446

Citation

Liu, Yixiang, et al. "Inhibitory Effect of Depolymerized Sulfated Galactans From Marine Red Algae On the Growth and Adhesion of Diarrheagenic Escherichia Coli." Marine Drugs, vol. 17, no. 12, 2019.
Liu Y, Liu W, Wang Y, et al. Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli. Mar Drugs. 2019;17(12).
Liu, Y., Liu, W., Wang, Y., Ma, Y., Huang, L., Zou, C., Li, D., Cao, M. J., & Liu, G. M. (2019). Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli. Marine Drugs, 17(12). https://doi.org/10.3390/md17120694
Liu Y, et al. Inhibitory Effect of Depolymerized Sulfated Galactans From Marine Red Algae On the Growth and Adhesion of Diarrheagenic Escherichia Coli. Mar Drugs. 2019 Dec 10;17(12) PubMed PMID: 31835446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli. AU - Liu,Yixiang, AU - Liu,Wenqiang, AU - Wang,Yanbo, AU - Ma,Yu, AU - Huang,Ling, AU - Zou,Chao, AU - Li,Donghui, AU - Cao,Min-Jie, AU - Liu,Guang-Ming, Y1 - 2019/12/10/ PY - 2019/11/03/received PY - 2019/12/03/revised PY - 2019/12/07/accepted PY - 2019/12/15/entrez PY - 2019/12/15/pubmed PY - 2020/5/14/medline KW - anti-adhesion KW - anti-diarrhea KW - enterotoxigenic Escherichia coli KW - marine algae KW - sulfated galactans JF - Marine drugs JO - Mar Drugs VL - 17 IS - 12 N2 - Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88. A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88. SN - 1660-3397 UR - https://www.unboundmedicine.com/medline/citation/31835446/Inhibitory_Effect_of_Depolymerized_Sulfated_Galactans_from_Marine_Red_Algae_on_the_Growth_and_Adhesion_of_Diarrheagenic_Escherichia_coli_ DB - PRIME DP - Unbound Medicine ER -