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Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR from Omeprazole Responsive Multidrug-Resistant Escherichia coli Isolates Causing Urinary Tract Infections.
Microbiol Insights 2019; 12:1178636119889629MI

Abstract

Antimicrobial resistance poses a threat in the treatment of infectious diseases in Bangladesh as well as in the world. Multidrug-resistant (MDR) Enterobacteriaceae, the most common cause of one such infectious disease, urinary tract infection (UTI), has contributed to the escalating problem of selecting empiric antibiotics against UTIs. The aim of this study was to investigate the presence of the efflux pump in MDR Escherichia coli isolates from UTI in the North-East region of Bangladesh, to isolate and characterize the AcrAB-TolC efflux pump genes of these locally isolated strains and to do mutation analysis of the efflux pump repressor AcrR gene to understand the AcrAB-TolC efflux pump mechanism. In the presence of omeprazole, an efflux pump inhibitor, every MDR E. coli isolate showed increased susceptibility to at least 1 of the 7 antibiotics investigated, indicating that efflux pump might be involved in their antibiotic resistance. Omeprazole decreased the minimum inhibitory concentration of every antibiotics being investigated by 2- to 8-fold. DNA and the deduced amino acid sequences of the polymerase chain reaction (PCR) products analyzed by bioinformatics tools revealed that the chromosomal AcrAB-TolC and AcrR genes were present in all MDR and antibiotic-susceptible E. coli isolates. However, the deduced amino acid sequences of the amplification refractory mutation system (ARMS) PCR product of the AcrR gene revealed that the substitution of arginine to cysteine at position 45 of AcrR was observed only in the MDR E. coli whose antibiotic susceptibility increased in the presence of omeprazole. Data reported herein support the notion that the increased antibiotic susceptibility of the MDR E. coli isolates in the presence of omeprazole might be due to efflux pump(s) inhibition and the AcrAB-TolC efflux pump might be a contributor to antibiotic resistance when the mutation of arginine to cysteine occurs at position 45 of AcrR.

Authors+Show Affiliations

Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.Department of Genetic Engineering & Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, Bangladesh.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31839709

Citation

Chowdhury, Nandan, et al. "Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR From Omeprazole Responsive Multidrug-Resistant Escherichia Coli Isolates Causing Urinary Tract Infections." Microbiology Insights, vol. 12, 2019, p. 1178636119889629.
Chowdhury N, Suhani S, Purkaystha A, et al. Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR from Omeprazole Responsive Multidrug-Resistant Escherichia coli Isolates Causing Urinary Tract Infections. Microbiol Insights. 2019;12:1178636119889629.
Chowdhury, N., Suhani, S., Purkaystha, A., Begum, M. K., Raihan, T., Alam, M. J., ... Azad, A. K. (2019). Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR from Omeprazole Responsive Multidrug-Resistant Escherichia coli Isolates Causing Urinary Tract Infections. Microbiology Insights, 12, p. 1178636119889629. doi:10.1177/1178636119889629.
Chowdhury N, et al. Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR From Omeprazole Responsive Multidrug-Resistant Escherichia Coli Isolates Causing Urinary Tract Infections. Microbiol Insights. 2019;12:1178636119889629. PubMed PMID: 31839709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR from Omeprazole Responsive Multidrug-Resistant Escherichia coli Isolates Causing Urinary Tract Infections. AU - Chowdhury,Nandan, AU - Suhani,Sabrina, AU - Purkaystha,Auditi, AU - Begum,Musammat Kulsuma, AU - Raihan,Topu, AU - Alam,Md Jahangir, AU - Islam,Kamrul, AU - Azad,Abul Kalam, Y1 - 2019/12/04/ PY - 2019/09/29/received PY - 2019/10/22/accepted PY - 2019/12/17/entrez PY - 2019/12/17/pubmed PY - 2019/12/17/medline KW - AcrAB-TolC KW - AcrR KW - MIC KW - Multidrug resistant KW - UTI KW - efflux KW - mutation SP - 1178636119889629 EP - 1178636119889629 JF - Microbiology insights JO - Microbiol Insights VL - 12 N2 - Antimicrobial resistance poses a threat in the treatment of infectious diseases in Bangladesh as well as in the world. Multidrug-resistant (MDR) Enterobacteriaceae, the most common cause of one such infectious disease, urinary tract infection (UTI), has contributed to the escalating problem of selecting empiric antibiotics against UTIs. The aim of this study was to investigate the presence of the efflux pump in MDR Escherichia coli isolates from UTI in the North-East region of Bangladesh, to isolate and characterize the AcrAB-TolC efflux pump genes of these locally isolated strains and to do mutation analysis of the efflux pump repressor AcrR gene to understand the AcrAB-TolC efflux pump mechanism. In the presence of omeprazole, an efflux pump inhibitor, every MDR E. coli isolate showed increased susceptibility to at least 1 of the 7 antibiotics investigated, indicating that efflux pump might be involved in their antibiotic resistance. Omeprazole decreased the minimum inhibitory concentration of every antibiotics being investigated by 2- to 8-fold. DNA and the deduced amino acid sequences of the polymerase chain reaction (PCR) products analyzed by bioinformatics tools revealed that the chromosomal AcrAB-TolC and AcrR genes were present in all MDR and antibiotic-susceptible E. coli isolates. However, the deduced amino acid sequences of the amplification refractory mutation system (ARMS) PCR product of the AcrR gene revealed that the substitution of arginine to cysteine at position 45 of AcrR was observed only in the MDR E. coli whose antibiotic susceptibility increased in the presence of omeprazole. Data reported herein support the notion that the increased antibiotic susceptibility of the MDR E. coli isolates in the presence of omeprazole might be due to efflux pump(s) inhibition and the AcrAB-TolC efflux pump might be a contributor to antibiotic resistance when the mutation of arginine to cysteine occurs at position 45 of AcrR. SN - 1178-6361 UR - https://www.unboundmedicine.com/medline/citation/31839709/Identification_of_AcrAB_TolC_Efflux_Pump_Genes_and_Detection_of_Mutation_in_Efflux_Repressor_AcrR_from_Omeprazole_Responsive_Multidrug_Resistant_Escherichia_coli_Isolates_Causing_Urinary_Tract_Infections_ L2 - http://journals.sagepub.com/doi/full/10.1177/1178636119889629?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -