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Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae).
J Invertebr Pathol 2019; 170:107302JI

Abstract

In this study, the first microsporidian pathogen from Altica hampei (Coleoptera: Chrysomelidae) is described based on light microscopy, ultrastructural characteristics and comparative 16S SSU rDNA analysis. All developmental stages of the microsporidium are diplokaryotic and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.82 ± 0.35 μm in length and 2.54 ± 0.27 μm in width. The polar filament of the binucleate spores is isofilar with 12-14 coils. Coils are 140.28 ± 4.88 nm (135.59-147.06; n = 36) in diameter and consist of six concentric layers of different electron density and thickness. The spores have a relatively thick (161.72 ± 29.19 nm) trilaminar spore wall. Morphological, ultrastructural and molecular features indicate that the described microsporidium belongs to the genus Nosema and is named Nosema alticae sp. nov.

Authors+Show Affiliations

Giresun University, Turkey.Selcuk University, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31843540

Citation

Yıldırım, Hilal, and Çağrı Bekircan. "Ultrastructural and Molecular Characterization of Nosema Alticae Sp. Nov. (Microsporidia: Nosematidae), Pathogen of the Flea Beetle, Altica Hampei Allard, 1867 (Coleoptera: Chrysomelidae)." Journal of Invertebrate Pathology, vol. 170, 2019, p. 107302.
Yıldırım H, Bekircan Ç. Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae). J Invertebr Pathol. 2019;170:107302.
Yıldırım, H., & Bekircan, Ç. (2019). Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae). Journal of Invertebrate Pathology, 170, p. 107302. doi:10.1016/j.jip.2019.107302.
Yıldırım H, Bekircan Ç. Ultrastructural and Molecular Characterization of Nosema Alticae Sp. Nov. (Microsporidia: Nosematidae), Pathogen of the Flea Beetle, Altica Hampei Allard, 1867 (Coleoptera: Chrysomelidae). J Invertebr Pathol. 2019 Dec 14;170:107302. PubMed PMID: 31843540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructural and molecular characterization of Nosema alticae sp. nov. (Microsporidia: Nosematidae), pathogen of the flea beetle, Altica hampei Allard, 1867 (Coleoptera: Chrysomelidae). AU - Yıldırım,Hilal, AU - Bekircan,Çağrı, Y1 - 2019/12/14/ PY - 2019/07/08/received PY - 2019/11/23/revised PY - 2019/12/04/accepted PY - 2019/12/18/pubmed PY - 2019/12/18/medline PY - 2019/12/18/entrez KW - Altica hampei KW - Chrysomelidae KW - Diplokaryotic KW - Microsporidia KW - Nosema KW - Pathogen SP - 107302 EP - 107302 JF - Journal of invertebrate pathology JO - J. Invertebr. Pathol. VL - 170 N2 - In this study, the first microsporidian pathogen from Altica hampei (Coleoptera: Chrysomelidae) is described based on light microscopy, ultrastructural characteristics and comparative 16S SSU rDNA analysis. All developmental stages of the microsporidium are diplokaryotic and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.82 ± 0.35 μm in length and 2.54 ± 0.27 μm in width. The polar filament of the binucleate spores is isofilar with 12-14 coils. Coils are 140.28 ± 4.88 nm (135.59-147.06; n = 36) in diameter and consist of six concentric layers of different electron density and thickness. The spores have a relatively thick (161.72 ± 29.19 nm) trilaminar spore wall. Morphological, ultrastructural and molecular features indicate that the described microsporidium belongs to the genus Nosema and is named Nosema alticae sp. nov. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/31843540/Ultrastructural_and_molecular_characterization_of_Nosema_alticae_sp._nov._(Microsporidia:_Nosematidae),_pathogen_of_the_flea_beetle,_Altica_hampei_Allard,_1867_(Coleoptera:_Chrysomelidae) L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(19)30192-2 DB - PRIME DP - Unbound Medicine ER -