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QTL mapping for grain yield-related traits in bread wheat via SNP-based selective genotyping.
Theor Appl Genet. 2020 Mar; 133(3):857-872.TA

Abstract

KEY MESSAGE

We identified four chromosome regions harboring QTL for grain yield-related traits, and breeder-friendly KASP markers were developed and validated for marker-assisted selection. Identification of major stable quantitative trait loci (QTL) for grain yield-related traits is important for yield potential improvement in wheat breeding. In the present study, 266 recombinant inbred lines (RILs) derived from a cross between Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895) were evaluated for thousand grain weight (TGW), grain length (GL), grain width (GW), and grain number per spike (GNS) in 10 environments and for grain filling rate in six environments. Sixty RILs, with 30 higher and 30 lower TGW, respectively, were genotyped using the wheat 660 K SNP array for preliminary QTL mapping. Four genetic regions on chromosomes 1AL, 2BS, 3AL, and 5B were identified to have a significant effect on TGW-related traits. A set of Kompetitive Allele Specific PCR markers were converted from the SNP markers on the above target chromosomes and used to genotype all 266 RILs. The mapping results confirmed the QTL named Qgw.caas-1AL, Qgl.caas-3AL, Qtgw.caas-5B, and Qgl.caas-5BS on the targeted chromosomes, explaining 5.0-20.6%, 5.7-15.7%, 5.5-17.3%, and 12.5-20.5% of the phenotypic variation for GW, GL, TGW, and GL, respectively. A novel major QTL for GNS on chromosome 5BS, explaining 5.2-15.2% of the phenotypic variation, was identified across eight environments. These QTL were further validated using BC1F4 populations derived from backcrosses ZM871/ZM895//ZM871 (121 lines) and ZM871/ZM895//ZM895 (175 lines) and 186 advanced breeding lines. Collectively, selective genotyping is a simple, economic, and effective approach for rapid QTL mapping and can be generally applied to genetic mapping studies for important agronomic traits.

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Authors+Show Affiliations

Yang L
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Zhao D
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Meng Z
Shangqiu Academy of Agricultural and Forestry Sciences, 10 Shengli Road, Shangqiu, 476000, Henan Province, China.
Xu K
Institute of Cotton Research, CAAS, 38 Huanghe Dadao, Anyang, 455000, Henan Province, China.
Yan J
Institute of Cotton Research, CAAS, 38 Huanghe Dadao, Anyang, 455000, Henan Province, China.
Xia X
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Cao S
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
Tian Y
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
He Z
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. International Maize and Wheat Improvement Center (CIMMYT), China Office, c/o CAAS, Beijing, 100081, China.
Zhang Y
Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. zhangyong05@caas.cn.

MeSH

AllelesChromosome MappingChromosomes, PlantCrosses, GeneticEdible GrainGenetic LinkageGenotypePhenotypePlant BreedingPolymorphism, Single NucleotideQuantitative Trait LociTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31844965

Citation

Yang, Li, et al. "QTL Mapping for Grain Yield-related Traits in Bread Wheat Via SNP-based Selective Genotyping." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 133, no. 3, 2020, pp. 857-872.
Yang L, Zhao D, Meng Z, et al. QTL mapping for grain yield-related traits in bread wheat via SNP-based selective genotyping. Theor Appl Genet. 2020;133(3):857-872.
Yang, L., Zhao, D., Meng, Z., Xu, K., Yan, J., Xia, X., Cao, S., Tian, Y., He, Z., & Zhang, Y. (2020). QTL mapping for grain yield-related traits in bread wheat via SNP-based selective genotyping. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 133(3), 857-872. https://doi.org/10.1007/s00122-019-03511-0
Yang L, et al. QTL Mapping for Grain Yield-related Traits in Bread Wheat Via SNP-based Selective Genotyping. Theor Appl Genet. 2020;133(3):857-872. PubMed PMID: 31844965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - QTL mapping for grain yield-related traits in bread wheat via SNP-based selective genotyping. AU - Yang,Li, AU - Zhao,Dehui, AU - Meng,Zili, AU - Xu,Kaijie, AU - Yan,Jun, AU - Xia,Xianchun, AU - Cao,Shuanghe, AU - Tian,Yubing, AU - He,Zhonghu, AU - Zhang,Yong, Y1 - 2019/12/16/ PY - 2019/03/24/received PY - 2019/12/11/accepted PY - 2019/12/18/pubmed PY - 2020/11/20/medline PY - 2019/12/18/entrez SP - 857 EP - 872 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 133 IS - 3 N2 - KEY MESSAGE: We identified four chromosome regions harboring QTL for grain yield-related traits, and breeder-friendly KASP markers were developed and validated for marker-assisted selection. Identification of major stable quantitative trait loci (QTL) for grain yield-related traits is important for yield potential improvement in wheat breeding. In the present study, 266 recombinant inbred lines (RILs) derived from a cross between Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895) were evaluated for thousand grain weight (TGW), grain length (GL), grain width (GW), and grain number per spike (GNS) in 10 environments and for grain filling rate in six environments. Sixty RILs, with 30 higher and 30 lower TGW, respectively, were genotyped using the wheat 660 K SNP array for preliminary QTL mapping. Four genetic regions on chromosomes 1AL, 2BS, 3AL, and 5B were identified to have a significant effect on TGW-related traits. A set of Kompetitive Allele Specific PCR markers were converted from the SNP markers on the above target chromosomes and used to genotype all 266 RILs. The mapping results confirmed the QTL named Qgw.caas-1AL, Qgl.caas-3AL, Qtgw.caas-5B, and Qgl.caas-5BS on the targeted chromosomes, explaining 5.0-20.6%, 5.7-15.7%, 5.5-17.3%, and 12.5-20.5% of the phenotypic variation for GW, GL, TGW, and GL, respectively. A novel major QTL for GNS on chromosome 5BS, explaining 5.2-15.2% of the phenotypic variation, was identified across eight environments. These QTL were further validated using BC1F4 populations derived from backcrosses ZM871/ZM895//ZM871 (121 lines) and ZM871/ZM895//ZM895 (175 lines) and 186 advanced breeding lines. Collectively, selective genotyping is a simple, economic, and effective approach for rapid QTL mapping and can be generally applied to genetic mapping studies for important agronomic traits. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/31844965/QTL_mapping_for_grain_yield_related_traits_in_bread_wheat_via_SNP_based_selective_genotyping_ L2 - https://dx.doi.org/10.1007/s00122-019-03511-0 DB - PRIME DP - Unbound Medicine ER -