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Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.
Histochem Cell Biol. 2020 Mar; 153(3):153-164.HC

Abstract

The objective of this study is to investigate the expression of enzymes involved in the sulfation of articular cartilage from proximal metacarpophalangeal (PMC) joint cartilage and distal metacarpophalangeal (DMC) joint cartilage in children with Kashin-Beck disease (KBD). The finger cartilage samples of PMC and DMC were collected from KBD and normal children aged 5-14 years old. Hematoxylin and eosin staining as well as immunohistochemical staining were used to observe the morphology and quantitate the expression of carbohydrate sulfotransferase 3 (CHST-3), carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), uronyl 2-O-sulfotransferase (UST), and aggrecan. In the results, the numbers of chondrocyte decreased in all three zones of PMC and DMC in the KBD group. Less positive staining cells for CHST-3, CHST-12, CHST-13, UST, and aggrecan were observed in almost all three zones of PMC and DMC in KBD. The positive staining cell rates of CHST-12 were higher in superficial and middle zones of PMC and DMC in KBD, and a significantly higher rate of CHST-13 was observed only in superficial zone of PMC in KBD. In conclusion, the abnormal expression of chondroitin sulfate sulfotransferases in chondrocytes of KBD children may provide an explanation for the cartilage damage, and provide therapeutic targets for the treatment.

Authors+Show Affiliations

School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, P. R. China.Shenzhen Institute, Xi'an Jiaotong University, Shenzhen, 518057, P. R. China. Department of Ophthalmology, The First Affiliated Hospital, Health Science Center of Xi'an Jiaotong University, Xi'an, 710061, P. R. China.School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, P. R. China.School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, P. R. China. Shenzhen Institute, Xi'an Jiaotong University, Shenzhen, 518057, P. R. China.School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, P. R. China.School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, P. R. China.School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, 710061, Shaanxi, P. R. China. Department of Integrative Medical Biology, Umeå University, 90187, Umeå, Sweden.Shenzhen Institute, Xi'an Jiaotong University, Shenzhen, 518057, P. R. China. bbbishop@126.com.Department of Integrative Medical Biology, Umeå University, 90187, Umeå, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31845005

Citation

Lei, Jian, et al. "Abnormal Expression of Chondroitin Sulfate Sulfotransferases in the Articular Cartilage of Pediatric Patients With Kashin-Beck Disease." Histochemistry and Cell Biology, vol. 153, no. 3, 2020, pp. 153-164.
Lei J, Yan S, Zhou Y, et al. Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease. Histochem Cell Biol. 2020;153(3):153-164.
Lei, J., Yan, S., Zhou, Y., Wang, L., Zhang, J., Guo, X., Lammi, M. J., Han, J., & Qu, C. (2020). Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease. Histochemistry and Cell Biology, 153(3), 153-164. https://doi.org/10.1007/s00418-019-01833-0
Lei J, et al. Abnormal Expression of Chondroitin Sulfate Sulfotransferases in the Articular Cartilage of Pediatric Patients With Kashin-Beck Disease. Histochem Cell Biol. 2020;153(3):153-164. PubMed PMID: 31845005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease. AU - Lei,Jian, AU - Yan,Siqi, AU - Zhou,Yuan, AU - Wang,Liyun, AU - Zhang,Jinghua, AU - Guo,Xiong, AU - Lammi,Mikko J, AU - Han,Jing, AU - Qu,Chengjuan, Y1 - 2019/12/16/ PY - 2019/11/27/accepted PY - 2019/12/18/pubmed PY - 2019/12/18/medline PY - 2019/12/18/entrez KW - Children’s cartilage KW - Chondroitin sulfate KW - Kashin–Beck disease KW - Selenium deficiency KW - Sulfation KW - Sulfotransferases SP - 153 EP - 164 JF - Histochemistry and cell biology JO - Histochem. Cell Biol. VL - 153 IS - 3 N2 - The objective of this study is to investigate the expression of enzymes involved in the sulfation of articular cartilage from proximal metacarpophalangeal (PMC) joint cartilage and distal metacarpophalangeal (DMC) joint cartilage in children with Kashin-Beck disease (KBD). The finger cartilage samples of PMC and DMC were collected from KBD and normal children aged 5-14 years old. Hematoxylin and eosin staining as well as immunohistochemical staining were used to observe the morphology and quantitate the expression of carbohydrate sulfotransferase 3 (CHST-3), carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), uronyl 2-O-sulfotransferase (UST), and aggrecan. In the results, the numbers of chondrocyte decreased in all three zones of PMC and DMC in the KBD group. Less positive staining cells for CHST-3, CHST-12, CHST-13, UST, and aggrecan were observed in almost all three zones of PMC and DMC in KBD. The positive staining cell rates of CHST-12 were higher in superficial and middle zones of PMC and DMC in KBD, and a significantly higher rate of CHST-13 was observed only in superficial zone of PMC in KBD. In conclusion, the abnormal expression of chondroitin sulfate sulfotransferases in chondrocytes of KBD children may provide an explanation for the cartilage damage, and provide therapeutic targets for the treatment. SN - 1432-119X UR - https://www.unboundmedicine.com/medline/citation/31845005/Abnormal_expression_of_chondroitin_sulfate_sulfotransferases_in_the_articular_cartilage_of_pediatric_patients_with_Kashin-Beck_disease L2 - https://dx.doi.org/10.1007/s00418-019-01833-0 DB - PRIME DP - Unbound Medicine ER -
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