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RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.).

Abstract

Pentatricopeptide repeat (PPR) proteins are one of the largest protein families in land plants. PPR proteins exhibit sequence-specific RNA-binding activity and are implicated in plant growth and development related processes. In this study, we report that the radicleless 1 (rl1) mutant in rice (Oryza sativa L.) exhibited defective radicle emergence in embryos and compromised grain filling in endosperms. Gene cloning and confirmation via genetic complementation analyses showed that RL1 encodes a P-type PPR protein, which is localized to mitochondria. The RL1 protein was specifically involved in the splicing of intron 1 of the mitochondrial nad4 transcript, which encodes a subunit of the mitochondrial NADH dehydrogenase complex. Consistent with this observation, the rl1 mutant exhibited altered mitochondrial morphology and lower ATP accumulation compared with the wild type. Thus, our findings suggest that RL1-mediated nad4 splicing is crucial for embryo and endosperm development in rice.

Authors+Show Affiliations

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742, USA.Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100093, China. Electronic address: cmliu@ibcas.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

31848048

Citation

Wu, Ming-Wei, et al. "RADICLELESS 1 (RL1)-mediated Nad4 Intron 1 Splicing Is Crucial for Embryo and Endosperm Development in Rice (Oryza Sativa L.)." Biochemical and Biophysical Research Communications, 2019.
Wu MW, Zhao H, Zhang JD, et al. RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.). Biochem Biophys Res Commun. 2019.
Wu, M. W., Zhao, H., Zhang, J. D., Guo, L., & Liu, C. M. (2019). RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.). Biochemical and Biophysical Research Communications, doi:10.1016/j.bbrc.2019.11.084.
Wu MW, et al. RADICLELESS 1 (RL1)-mediated Nad4 Intron 1 Splicing Is Crucial for Embryo and Endosperm Development in Rice (Oryza Sativa L.). Biochem Biophys Res Commun. 2019 Dec 14; PubMed PMID: 31848048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RADICLELESS 1 (RL1)-mediated nad4 intron 1 splicing is crucial for embryo and endosperm development in rice (Oryza sativa L.). AU - Wu,Ming-Wei, AU - Zhao,Heng, AU - Zhang,Jin-Dan, AU - Guo,Lei, AU - Liu,Chun-Ming, Y1 - 2019/12/14/ PY - 2019/11/13/received PY - 2019/11/14/accepted PY - 2019/12/19/entrez KW - Mitochondrial morphology KW - Pentatricopeptide repeat KW - RL1 KW - nad4 KW - splicing JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. N2 - Pentatricopeptide repeat (PPR) proteins are one of the largest protein families in land plants. PPR proteins exhibit sequence-specific RNA-binding activity and are implicated in plant growth and development related processes. In this study, we report that the radicleless 1 (rl1) mutant in rice (Oryza sativa L.) exhibited defective radicle emergence in embryos and compromised grain filling in endosperms. Gene cloning and confirmation via genetic complementation analyses showed that RL1 encodes a P-type PPR protein, which is localized to mitochondria. The RL1 protein was specifically involved in the splicing of intron 1 of the mitochondrial nad4 transcript, which encodes a subunit of the mitochondrial NADH dehydrogenase complex. Consistent with this observation, the rl1 mutant exhibited altered mitochondrial morphology and lower ATP accumulation compared with the wild type. Thus, our findings suggest that RL1-mediated nad4 splicing is crucial for embryo and endosperm development in rice. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/31848048/RADICLELESS_1_(RL1)-mediated_nad4_intron_1_splicing_is_crucial_for_embryo_and_endosperm_development_in_rice_(Oryza_sativa_L.) L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(19)32210-7 DB - PRIME DP - Unbound Medicine ER -